Self-Healing Conductive Injectable Hydrogels with Antibacterial Activity as Cell Delivery Carrier for Cardiac Cell Therapy

ACS Applied Materials and Interfaces

Volumn 8, Issue 27, 2016, Pages 17138-17150

  Dong, Ruonan ^a   Zhao, Xin ^a   Guo, Baolin ^a   Ma, Peter X ^a,b  

^a XI'AN JIAOTONG UNIVERSITY   (China)

^b UNIVERSITY OF MICHIGAN   (United States)

Author keywords

cell therapy; conducting polymer; injectable hydrogels; myocardial infarction; self healing

Indexed keywords

BIOCOMPATIBILITY; BIODEGRADABILITY; CARDIOLOGY; CELLS; CONDUCTING POLYMERS; CYTOLOGY; HEART; POLYETHYLENE GLYCOLS; POLYETHYLENE OXIDES; TISSUE; VEHICLES;

ANTI-BACTERIAL ACTIVITY; ANTIBACTERIAL PROPERTIES; CELL THERAPY; ELECTROACTIVE HYDROGELS; INJECTABLE HYDROGELS; MYOCARDIAL INFARCTION; PHYSIOLOGICAL CONDITION; SELF-HEALING;

HYDROGELS;

ANTIINFECTIVE AGENT; CHITOSAN; DRUG CARRIER;

BIOLOGICAL THERAPY; CHEMISTRY; HEART; HYDROGEL;

ANTI-BACTERIAL AGENTS; CELL- AND TISSUE-BASED THERAPY; CHITOSAN; DRUG CARRIERS; HEART; HYDROGELS;

EID: 84978372428     PISSN: 19448244     EISSN: 19448252     Source Type: Journal    
DOI: 10.1021/acsami.6b04911     Document Type: Article

References (76)

1. Hastings, C. L.; Roche, E. T.; Ruiz-Hernandez, E.; Schenke-Layland, K.; Walsh, C. J.; Duffy, G. P. Drug and Cell Delivery for Cardiac Regeneration Adv. Drug Delivery Rev. 2015, 84, 85-106 10.1016/j.addr.2014.08.006
2. Wei, H.; Ooi, T. H.; Tan, G.; Lim, S. Y.; Qian, L.; Wong, P.; Shim, W. Cell Delivery and Tracking in Post-Myocardial Infarction Cardiac Stem Cell Therapy: An Introduction for Clinical Researchers Heart Failure Rev. 2010, 15, 1-14 10.1007/s10741-009-9134-1
3. Liu, Q.; Tian, S.; Zhao, C.; Chen, X.; Lei, I.; Wang, Z.; Ma, P. X. Porous Nanofibrous Poly (L-Lactic Acid) Scaffolds Supporting Cardiovascular Progenitor Cells for Cardiac Tissue Engineering Acta Biomater. 2015, 26, 105-114 10.1016/j.actbio.2015.08.017
4. Hodgson, D. M.; Behfar, A.; Zingman, L. V.; Kane, G. C.; Perez-Terzic, C.; Alekseev, A. E.; Pucéat, M.; Terzic, A. Stable Benefit of Embryonic Stem Cell Therapy in Myocardial Infarction Am. J. Physiol.: Heart Circ. Physiol. 2004, 287, H471-H479
5. Miyahara, Y.; Nagaya, N.; Kataoka, M.; Yanagawa, B.; Tanaka, K.; Hao, H.; Ishino, K.; Ishida, H.; Shimizu, T.; Kangawa, K. et al. Monolayered Mesenchymal Stem Cells Repair Scarred Myocardium after Myocardial Infarction Nat. Med. 2006, 12, 459-465 10.1038/nm1391
6. Perin, E. C.; López, J. Methods of Stem Cell Delivery in Cardiac Diseases Nat. Clin. Pract. Cardiovasc. Med. 2006, 3, S110-S113 10.1038/ncpcardio0447
7. Mooney, D. J.; Vandenburgh, H. Cell Delivery Mechanisms for Tissue Repair Cell stem cell 2008, 2, 205-213 10.1016/j.stem.2008.02.005
8. Ghostine, S.; Carrion, C.; Souza, L. C. G.; Richard, P.; Bruneval, P.; Vilquin, J.-T.; Pouzet, B.; Schwartz, K.; Menasché, P.; Hagege, A. A. Long-Term Efficacy of Myoblast Transplantation on Regional Structure and Function after Myocardial Infarction Circulation 2002, 106, I-131-I-136
9. Pouzet, B.; Ghostine, S.; Vilquin, J.-T.; Garcin, I.; Scorsin, M.; Hagège, A. A.; Duboc, D.; Schwartz, K.; Menasché, P. Is Skeletal Myoblast Transplantation Clinically Relevant in the Era of Angiotensin-Converting Enzyme Inhibitors? Circulation 2001, 104, I-223-I-228 10.1161/hc37t1.094593
10. Suzuki, K.; Murtuza, B.; Fukushima, S.; Smolenski, R. T.; Varela-Carver, A.; Coppen, S. R.; Yacoub, M. H. Targeted Cell Delivery into Infarcted Rat Hearts by Retrograde Intracoronary Infusion: Distribution, Dynamics, and Influence on Cardiac Function Circulation 2004, 110, II-225-II-230 10.1161/01.CIR.0000138191.11580.e3
11. Kudo, M.; Wang, Y.; Wani, M. A.; Xu, M.; Ayub, A.; Ashraf, M. Implantation of Bone Marrow Stem Cells Reduces the Infarction and Fibrosis in Ischemic Mouse Heart J. Mol. Cell. Cardiol. 2003, 35, 1113-1119 10.1016/S0022-2828(03)00211-6
12. Haenel, A.; Ghosn, M.; Schulz, D. G.; Vykoukal, J.; Shah, D.; Raizner, A. E.; Alt, E. Fresh Adipose Tissue Derived Stem Cells Significantly Enhance Ventricular Function in a Chronic Porcine Myocardial Infarction Model J. Am. Coll. Cardiol. 2015, 65, A1911-A1911 10.1016/S0735-1097(15)61911-8
13. Hong, S. J.; Rogers, P. I.; Kihlken, J.; Warfel, J.; Bull, C.; Deuter-Reinhard, M.; Feng, D.; Xie, J.; Kyle, A.; Merfeld-Clauss, S. et al. Intravenous Xenogeneic Transplantation of Human Adipose-Derived Stem Cells Improves Left Ventricular Function and Microvascular Integrity in Swine Myocardial Infarction Model Catheter. Cardio. Inte. 2015, 86, E38-E48 10.1002/ccd.25566
14. Shin, J.; Lee, J. S.; Lee, C.; Park, H. J.; Yang, K.; Jin, Y.; Ryu, J. H.; Hong, K. S.; Moon, S. H.; Chung, H. M. et al. Tissue Adhesive Catechol-Modified Hyaluronic Acid Hydrogel for Effective, Minimally Invasive Cell Therapy Adv. Funct. Mater. 2015, 25, 3814-3824 10.1002/adfm.201500006
15. Smith, R. R.; Marbán, E.; Marbán, L. Enhancing Retention and Efficacy of Cardiosphere-Derived Cells Administered after Myocardial Infarction Using a Hyaluronan-Gelatin Hydrogel Biomatter 2013, 3, e24490 10.4161/biom.24490
16. Wang, H.; Shi, J.; Wang, Y.; Yin, Y.; Wang, L.; Liu, J.; Liu, Z.; Duan, C.; Zhu, P.; Wang, C. Promotion of Cardiac Differentiation of Brown Adipose Derived Stem Cells by Chitosan Hydrogel for Repair after Myocardial Infarction Biomaterials 2014, 35, 3986-98 10.1016/j.biomaterials.2014.01.021
17. Janse, M. J.; Anderson, R. H.; van Capelle, F. J.; Durrer, D. A Combined Electrophysiological and Anatomical Study of the Human Fetal Heart Am. Heart J. 1976, 91, 556-562 10.1016/S0002-8703(76)80139-1
18. Anderson, R. H.; Durrer, D.; JANSE, M. J.; VAN CAPELLE, F. J.; BILLETE, J.; BECKER, A. E.; DURRER, D. A Combined Morphological and Electrophysiological Study of the Atrioventricular Node of the Rabbit Heart Circ. Res. 1974, 35, 909-922 10.1161/01.RES.35.6.909
19. Woo, D. G.; Shim, M.-S.; Park, J. S.; Yang, H. N.; Lee, D.-R.; Park, K.-H. The Effect of Electrical Stimulation on the Differentiation of Hescs Adhered onto Fibronectin-Coated Gold Nanoparticles Biomaterials 2009, 30, 5631-5638 10.1016/j.biomaterials.2009.07.026
20. Serena, E.; Figallo, E.; Tandon, N.; Cannizzaro, C.; Gerecht, S.; Elvassore, N.; Vunjak-Novakovic, G. Electrical Stimulation of Human Embryonic Stem Cells: Cardiac Differentiation and the Generation of Reactive Oxygen Species Exp. Cell Res. 2009, 315, 3611-3619 10.1016/j.yexcr.2009.08.015
21. Xie, M. H.; Wang, L.; Guo, B. L.; Wang, Z.; Chen, Y. E.; Ma, P. X. Ductile Electroactive Biodegradable Hyperbranched Polylactide Copolymers Enhancing Myoblast Differentiation Biomaterials 2015, 71, 158-167 10.1016/j.biomaterials.2015.08.042
22. Shuai, C.; Deng, J.; Gao, C.; Feng, P.; Peng, S.; Xiao, T.; Deng, Y. Mechanisms of Tetraneedlelike Zno Whiskers Reinforced Forsterite/Bioglass Scaffolds J. Alloys Compd. 2015, 636, 341-347 10.1016/j.jallcom.2015.02.077
23. Mawad, D.; Stewart, E.; Officer, D. L.; Romeo, T.; Wagner, P.; Wagner, K.; Wallace, G. G. A Single Component Conducting Polymer Hydrogel as a Scaffold for Tissue Engineering Adv. Funct. Mater. 2012, 22, 2692-2699 10.1002/adfm.201102373
24. Tseng, T. C.; Tao, L.; Hsieh, F. Y.; Wei, Y.; Chiu, I. M.; Hsu, S. h. An Injectable, Self-Healing Hydrogel to Repair the Central Nervous System Adv. Mater. 2015, 27, 3518-3524 10.1002/adma.201500762
25. Guo, B.; Sun, Y.; Finne-Wistrand, A.; Mustafa, K.; Albertsson, A.-C. Electroactive Porous Tubular Scaffolds with Degradability and Non-Cytotoxicity for Neural Tissue Regeneration Acta Biomater. 2012, 8, 144-153 10.1016/j.actbio.2011.09.027
26. Baheiraei, N.; Yeganeh, H.; Ai, J.; Gharibi, R.; Azami, M.; Faghihi, F. Synthesis, Characterization and Antioxidant Activity of a Novel Electroactive and Biodegradable Polyurethane for Cardiac Tissue Engineering Application Mater. Sci. Eng., C 2014, 44, 24-37 10.1016/j.msec.2014.07.061
27. Chen, P.-H.; Liao, H.-C.; Hsu, S.-H.; Chen, R.-S.; Wu, M.-C.; Yang, Y.-F.; Wu, C.-C.; Chen, M.-H.; Su, W.-F. A Novel Polyurethane/Cellulose Fibrous Scaffold for Cardiac Tissue Engineering RSC Adv. 2015, 5, 6932-6939 10.1039/C4RA12486C
28. Shin, S. R.; Jung, S. M.; Zalabany, M.; Kim, K.; Zorlutuna, P.; Kim, S. b.; Nikkhah, M.; Khabiry, M.; Azize, M.; Kong, J. et al. Carbon-Nanotube-Embedded Hydrogel Sheets for Engineering Cardiac Constructs and Bioactuators ACS Nano 2013, 7, 2369-2380 10.1021/nn305559j
29. Bidez, P. R.; Li, S.; MacDiarmid, A. G.; Venancio, E. C.; Wei, Y.; Lelkes, P. I. Polyaniline, an Electroactive Polymer, Supports Adhesion and Proliferation of Cardiac Myoblasts J. Biomater. Sci., Polym. Ed. 2006, 17, 199-212 10.1163/156856206774879180
30. Kotanen, C. N.; Wilson, A. N.; Dong, C.; Dinu, C.-Z.; Justin, G. A.; Guiseppi-Elie, A. The Effect of the Physicochemical Properties of Bioactive Electroconductive Hydrogels on the Growth and Proliferation of Attachment Dependent Cells Biomaterials 2013, 34, 6318-6327 10.1016/j.biomaterials.2013.05.022
31. Mathur, A. B.; Collinsworth, A. M.; Reichert, W. M.; Kraus, W. E.; Truskey, G. A. Endothelial, Cardiac Muscle and Skeletal Muscle Exhibit Different Viscous and Elastic Properties as Determined by Atomic Force Microscopy J. Biomech. 2001, 34, 1545-1553 10.1016/S0021-9290(01)00149-X
32. Irving, T.; Granzier, H. L. Passive Tension in Cardiac Muscle: Contribution of Collagen, Titin, Microtubules, and Intermediate Filaments Biophys. J. 1995, 68, 1027-1044 10.1016/S0006-3495(95)80278-X
33. Wool, R. P. Self-Healing Materials: A Review Soft Matter 2008, 4, 400-418 10.1039/b711716g
34. Zhao, X.; Guo, B. L.; Ma, P. X. Single Component Thermo-Gelling Electroactive Hydrogels from Poly(Caprolactone)-Poly(Ethylene Glycol)-Poly(Caprolactone)-Graft-Aniline Tetramer Amphiphilic Copolymers J. Mater. Chem. B 2015, 3, 8459-8468 10.1039/C5TB01658D
35. Ma, X. J.; Ge, J.; Li, Y.; Guo, B. L.; Ma, P. X. Nanofibrous Electroactive Scaffolds from a Chitosan-Grafted-Aniline Tetramer by Electrospinning for Tissue Engineering RSC Adv. 2014, 4, 13652-13661 10.1039/c4ra00083h
36. Zhang, Y.; Tao, L.; Li, S.; Wei, Y. Synthesis of Multiresponsive and Dynamic Chitosan-Based Hydrogels for Controlled Release of Bioactive Molecules Biomacromolecules 2011, 12, 2894-2901 10.1021/bm200423f
37. Yeom, J.; Kim, S. J.; Jung, H.; Namkoong, H.; Yang, J.; Hwang, B. W.; Oh, K.; Kim, K.; Sung, Y. C.; Hahn, S. K. Supramolecular Hydrogels for Long-Term Bioengineered Stem Cell Therapy Adv. Healthcare Mater. 2015, 4, 237-244 10.1002/adhm.201400304
38. Zhao, X.; Li, P.; Guo, B.; Ma, P. X. Antibacterial and Conductive Injectable Hydrogels Based on Quaternized Chitosan-Graft-Polyaniline/Oxidized Dextran for Tissue Engineering Acta Biomater. 2015, 26, 236-248 10.1016/j.actbio.2015.08.006
39. da Silva, C. M.; da Silva, D. L.; Modolo, L. V.; Alves, R. B.; de Resende, M. A.; Martins, C. V.; de Fátima, Â. Schiff Bases: A Short Review of Their Antimicrobial Activities J. Adv. Res. 2011, 2, 1-8 10.1016/j.jare.2010.05.004
40. Xin, Y.; Yuan, J. Y. Schiff's Base as a Stimuli-Responsive Linker in Polymer Chemistry Polym. Chem. 2012, 3, 3045-3055 10.1039/c2py20290e
41. Guo, B. L.; Yuan, J. F.; Gao, Q. Y. Ph and Ionic Sensitive Chitosan/Carboxymethyl Chitosan Ipn Complex Films for the Controlled Release of Coenzyme A Colloid Polym. Sci. 2008, 286, 175-181 10.1007/s00396-007-1749-y
42. Li, L.; Ge, J.; Guo, B.; Ma, P. X. In Situ Forming Biodegradable Electroactive Hydrogels Polym. Chem. 2014, 5, 2880-2890 10.1039/c3py01634j
43. Xie, M.; Wang, L.; Ge, J.; Guo, B.; Ma, P. X. Strong Electroactive Biodegradable Shape Memory Polymer Networks Based on Star-Shaped Polylactide and Aniline Trimer for Bone Tissue Engineering ACS Appl. Mater. Interfaces 2015, 7, 6772-6781 10.1021/acsami.5b00191
44. Surowiec, A.; Stuchly, S.; Eidus, L.; Swarup, A. In Vitro Dielectric Properties of Human Tissues at Radiofrequencies Phys. Med. Biol. 1987, 32, 615 10.1088/0031-9155/32/5/007
45. Keller, M. W.; White, S. R.; Sottos, N. R. A Self-Healing Poly (Dimethyl Siloxane) Elastomer Adv. Funct. Mater. 2007, 17, 2399-2404 10.1002/adfm.200700086
46. Hager, M. D.; Greil, P.; Leyens, C.; van der Zwaag, S.; Schubert, U. S. Self-Healing Materials Adv. Mater. 2010, 22, 5424-5430 10.1002/adma.201003036
47. Klabunde, R. E. Cardiovascular Physiology Concepts, 2 nd ed.; Lippincott Williams & Wilkins/Wolters Kluwer: Philadelphia, PA, 2012; p xi, 243 p.
48. Barker, P. C. A.; Houle, H.; Li, J. S.; Miller, S.; Herlong, J. R.; Camitta, M. G. W. Global Longitudinal Cardiac Strain and Strain Rate for Assessment of Fetal Cardiac Function: Novel Experience with Velocity Vector Imaging Echocardiogr-J. Card 2009, 26, 28-36 10.1111/j.1540-8175.2008.00761.x
49. Li, L. C.; Yu, M.; Ma, P. X.; Guo, B. L. Electroactive Degradable Copolymers Enhancing Osteogenic Differentiation from Bone Marrow Derived Mesenchymal Stem Cells J. Mater. Chem. B 2016, 4, 471-481 10.1039/C5TB01899D
50. Wu, Y.; Wang, L.; Guo, B.; Ma, P. X. Injectable Biodegradable Hydrogels and Microgels Based on Methacrylated Poly (Ethylene Glycol)-Co-Poly (Glycerol Sebacate) Multi-Block Copolymers: Synthesis, Characterization, and Cell Encapsulation J. Mater. Chem. B 2014, 2, 3674-3685 10.1039/c3tb21716g
51. Wang, L.; Wu, Y.; Guo, B.; Ma, P. X. Nanofiber Yarn/Hydrogel Core-Shell Scaffolds Mimicking Native Skeletal Muscle Tissue for Guiding 3d Myoblast Alignment, Elongation, and Differentiation ACS Nano 2015, 9, 9167-9179 10.1021/acsnano.5b03644
52. Chen, J.; Dong, R. N.; Ge, J.; Guo, B. L.; Ma, P. X. Biocompatible, Biodegradable, and Electroactive Polyurethane-Urea Elastomers with Tunable Hydrophilicity for Skeletal Muscle Tissue Engineering ACS Appl. Mater. Interfaces 2015, 7, 28273-28285 10.1021/acsami.5b10829
53. Wu, Y.; Wang, L.; Guo, B.; Shao, Y.; Ma, P. X. Electroactive Biodegradable Polyurethane Significantly Enhanced Schwann Cells Myelin Gene Expression and Neurotrophin Secretion for Peripheral Nerve Tissue Engineering Biomaterials 2016, 87, 18-31 10.1016/j.biomaterials.2016.02.010
54. Zhang, Q.; Yan, Y.; Li, S.; Feng, T. The Synthesis and Characterization of a Novel Biodegradable and Electroactive Polyphosphazene for Nerve Regeneration Mater. Sci. Eng., C 2010, 30, 160-166 10.1016/j.msec.2009.09.013
55. Cui, H.; Cui, L.; Zhang, P.; Huang, Y.; Wei, Y.; Chen, X. In Situ Electroactive and Antioxidant Supramolecular Hydrogel Based on Cyclodextrin/Copolymer Inclusion for Tissue Engineering Repair Macromol. Biosci. 2014, 14, 440-450 10.1002/mabi.201300366
56. Cui, H.; Shao, J.; Wang, Y.; Zhang, P.; Chen, X.; Wei, Y. Pla-Peg-Pla and Its Electroactive Tetraaniline Copolymer as Multi-Interactive Injectable Hydrogels for Tissue Engineering Biomacromolecules 2013, 14, 1904-1912 10.1021/bm4002766
57. Kai, D.; Prabhakaran, M. P.; Jin, G.; Ramakrishna, S. Polypyrrole-Contained Electrospun Conductive Nanofibrous Membranes for Cardiac Tissue Engineering J. Biomed. Mater. Res., Part A 2011, 99A, 376-385 10.1002/jbm.a.33200
58. Giraud, M. N.; Ayuni, E.; Cook, S.; Siepe, M.; Carrel, T. P.; Tevaearai, H. T. Hydrogel-Based Engineered Skeletal Muscle Grafts Normalize Heart Function Early after Myocardial Infarction Artif. Organs 2008, 32, 692-700 10.1111/j.1525-1594.2008.00595.x
59. Yang, B. G.; Yao, F. L.; Hao, T.; Fang, W. C.; Ye, L.; Zhang, Y. B.; Wang, Y.; Li, J. J.; Wang, C. Y. Development of Electrically Conductive Double-Network Hydrogels Via One-Step Facile Strategy for Cardiac Tissue Engineering Adv. Healthcare Mater. 2016, 5, 474-488 10.1002/adhm.201500520
60. Nam, H. Y.; McGinn, A.; Kim, P.-H.; Kim, S. W.; Bull, D. A. Primary Cardiomyocyte-Targeted Bioreducible Polymer for Efficient Gene Delivery to the Myocardium Biomaterials 2010, 31, 8081-8087 10.1016/j.biomaterials.2010.07.025
61. Taylor, D. A.; Atkins, B. Z.; Hungspreugs, P.; Jones, T. R.; Reedy, M. C.; Hutcheson, K. A.; Glower, D. D.; Kraus, W. E. Regenerating Functional Myocardium: Improved Performance after Skeletal Myoblast Transplantation Nat. Med. 1998, 4, 929-933 10.1038/nm0898-929
62. Goossens, H.; Ferech, M.; Stichele, R. V.; Elseviers, M.; Grp, E. P. Outpatient Antibiotic Use in Europe and Association with Resistance: A Cross-National Database Study Lancet 2005, 365, 579-587 10.1016/S0140-6736(05)70799-6
63. Neu, H. C. The Crisis in Antibiotic-Resistance Science 1992, 257, 1064-1073 10.1126/science.257.5073.1064
64. Zheng, F.; Wang, S.; Wen, S.; Shen, M.; Zhu, M.; Shi, X. Characterization and Antibacterial Activity of Amoxicillin-Loaded Electrospun Nano-Hydroxyapatite/Poly (Lactic-Co-Glycolic Acid) Composite Nanofibers Biomaterials 2013, 34, 1402-1412 10.1016/j.biomaterials.2012.10.071
65. Currie, H. A.; Deschaume, O.; Naik, R. R.; Perry, C. C.; Kaplan, D. L. Genetically Engineered Chimeric Silk-Silver Binding Proteins Adv. Funct. Mater. 2011, 21, 2889-2895 10.1002/adfm.201100249
66. Feng, K.; Sun, H. L.; Bradley, M. A.; Dupler, E. J.; Giannobile, W. V.; Ma, P. X. Novel Antibacterial Nanofibrous Plla Scaffolds J. Controlled Release 2010, 146, 363-369 10.1016/j.jconrel.2010.05.035
67. Fooladi, A. A. I.; Hosseini, H. M.; Hafezi, F.; Hosseinnejad, F.; Nourani, M. R. Sol-Gel-Derived Bioactive Glass Containing Sio2-Mgo-Cao-P2o5 as an Antibacterial Scaffold J. Biomed. Mater. Res., Part A 2013, 101A, 1582-1587 10.1002/jbm.a.34464
68. Song, A. R.; Rane, A. A.; Christman, K. L. Antibacterial and Cell-Adhesive Polypeptide and Poly(Ethylene Glycol) Hydrogel as a Potential Scaffold for Wound Healing Acta Biomater. 2012, 8, 41-50 10.1016/j.actbio.2011.10.004
69. Alt, V.; Bechert, T.; Steinrucke, P.; Wagener, M.; Seidel, P.; Dingeldein, E.; Domann, E.; Schnettler, R. An in Vitro Assessment of the Antibacterial Properties and Cytotoxicity of Nanoparticulate Silver Bone Cement Biomaterials 2004, 25, 4383-4391 10.1016/j.biomaterials.2003.10.078
70. Liu, X.; Song, L.; Li, L.; Li, S.; Yao, K. Antibacterial Effects of Chitosan and Its Water-Soluble Derivatives on E. Coli, Plasmids DNA, and Mrna J. Appl. Polym. Sci. 2007, 103, 3521-3528 10.1002/app.25421
71. Kucekova, Z.; Kasparkova, V.; Humpolicek, P.; Sevcikova, P.; Stejskal, J. Antibacterial Properties of Polyaniline-Silver Films Chem. Pap. 2013, 67, 1103-1108 10.2478/s11696-013-0385-x
72. Gizdavic-Nikolaidis, M. R.; Bennett, J. R.; Swift, S.; Easteal, A. J.; Ambrose, M. Broad Spectrum Antimicrobial Activity of Functionalized Polyanilines Acta Biomater. 2011, 7, 4204-4209 10.1016/j.actbio.2011.07.018
73. Gharibi, R.; Yeganeh, H.; Rezapour-Lactoee, A.; Hassan, Z. M. Stimulation of Wound Healing by Electroactive, Antibacterial, and Antioxidant Polyurethane/Siloxane Dressing Membranes: In Vitro and in Vivo Evaluations ACS Appl. Mater. Interfaces 2015, 7, 24296-24311 10.1021/acsami.5b08376
74. Gizdavic-Nikolaidis, M.; Ray, S.; Bennett, J.; Swift, S.; Bowmaker, G.; Easteal, A. Electrospun Poly (Aniline-Co-Ethyl 3-Aminobenzoate)/Poly (Lactic Acid) Nanofibers and Their Potential in Biomedical Applications J. Polym. Sci., Part A: Polym. Chem. 2011, 49, 4902-4910 10.1002/pola.24946
75. Guo, B. L.; Ma, P. X. Synthetic Biodegradable Functional Polymers for Tissue Engineering: A Brief Review Sci. China: Chem. 2014, 57, 490-500 10.1007/s11426-014-5086-y
76. Guo, B.; Glavas, L.; Albertsson, A.-C. Biodegradable and Electrically Conducting Polymers for Biomedical Applications Prog. Polym. Sci. 2013, 38, 1263-1286 10.1016/j.progpolymsci.2013.06.003