A new sensitive and quantitative chemiluminescent assay to monitor intracellular xanthine oxidase activity for rapid screening of inhibitors in living endothelial cells

Analytical and Bioanalytical Chemistry

Volumn 408, Issue 30, 2016, Pages 8755-8760

  Caliceti, C ^a,b   Calabria, D ^a   Roda, A ^a,b  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b NATIONAL INSTITUTE OF BIOSTRUCTURES AND BIOSYSTEMS   (Italy)

Author keywords

Chemiluminescence; Inhibitors; Quantitative intracellular assay; Vascular endothelial cells; Xanthine oxidase

Indexed keywords

BIOASSAY; CELLS; CHEMILUMINESCENCE; CORROSION INHIBITORS; CYTOLOGY; DIAGNOSIS; ORGANIC ACIDS;

ACTIVE METABOLITES; CARDIO-VASCULAR DISEASE; CHEMILUMINESCENCE ASSAY; DETECTION SYSTEM; LINEAR DECREASE; NATURAL PRODUCTS; VASCULAR ENDOTHELIAL CELLS; XANTHINE OXIDASE;

ENDOTHELIAL CELLS;

ALLOPURINOL; ENZYME INHIBITOR; LUMINOL; OXIPURINOL; URIC ACID; XANTHINE OXIDASE;

ANTAGONISTS AND INHIBITORS; BIOASSAY; CHEMISTRY; CYTOPLASM; DRUG EFFECTS; ENZYMOLOGY; HUMAN; KINETICS; LIMIT OF DETECTION; LUMINESCENCE; METABOLISM; OXIDATION REDUCTION REACTION; PROCEDURES; UMBILICAL VEIN ENDOTHELIAL CELL;

ALLOPURINOL; BIOLOGICAL ASSAY; CYTOPLASM; ENZYME INHIBITORS; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; HUMANS; KINETICS; LIMIT OF DETECTION; LUMINESCENT MEASUREMENTS; LUMINOL; OXIDATION-REDUCTION; OXYPURINOL; URIC ACID; XANTHINE OXIDASE;

EID: 84978161541     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-016-9763-x     Document Type: Article

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