Using Multiplex Molecular Testing to Determine the Etiology of Acute Gastroenteritis in Children

Journal of Pediatrics

Volumn 176, Issue , 2016, Pages 50-56.e2

  Nicholson, Maribeth R ^a   Van Horn, Gerald T ^b   Tang, Yi Wei ^c   Vinjé, Jan ^d   Payne, Daniel C ^d   Edwards, Kathryn M ^e   Chappell, James D ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b Department of Microbiology   (United States)

^c WEILL CORNELL MEDICAL COLLEGE   (United States)

^d CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

^e VANDERBILT UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Clostridium difficile; coinfections; colonization; diarrhea; norovirus

Indexed keywords

REAGENT;

ACUTE GASTROENTERITIS; ARTICLE; ASSAY; ASTROVIRIDAE; CAMPYLOBACTER; CHILD; CLINICAL FEATURE; CONTROLLED STUDY; DISEASE SURVEILLANCE; ESCHERICHIA COLI; FECES; FEMALE; HUMAN; INFANT; MAJOR CLINICAL STUDY; MALE; MOLECULAR DIAGNOSTICS; PEPTOCLOSTRIDIUM DIFFICILE; PRIORITY JOURNAL; PROSPECTIVE STUDY; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SALMONELLA; SAPOVIRUS; SHIGELLA; VIBRIO CHOLERAE; YERSINIA ENTEROCOLITICA; ACUTE DISEASE; GASTROENTERITIS; HEALTH SURVEY; MICROBIOLOGY; MOLECULAR DIAGNOSIS; MULTIPLEX POLYMERASE CHAIN REACTION; NEWBORN; PARASITOLOGY; PRESCHOOL CHILD; PROCEDURES;

ACUTE DISEASE; CHILD, PRESCHOOL; FEMALE; GASTROENTERITIS; HUMANS; INFANT; INFANT, NEWBORN; MALE; MOLECULAR DIAGNOSTIC TECHNIQUES; MULTIPLEX POLYMERASE CHAIN REACTION; POPULATION SURVEILLANCE; PROSPECTIVE STUDIES;

EID: 84977576575     PISSN: 00223476     EISSN: 10976833     Source Type: Journal    
DOI: 10.1016/j.jpeds.2016.05.068     Document Type: Article

References (34)

1. 1 Glass, R.I., Bresee, J., Jiang, B., Gentsch, J., Ando, T., Fankhauser, R., et al. Gastroenteritis viruses: an overview. Novartis Found Symp 238 (2001), 5–19.
2. 2 Matson, D.O., Estes, M.K., Impact of rotavirus infection at a large pediatric hospital. J Infect Dis 162 (1990), 598–607.
3. 3 Payne, D.C., Vinjé, J., Szilagyi, P.G., Edwards, K.M., Staat, M.A., Weinberg, G.A., et al. Norovirus and medically attended gastroenteritis in US children. N Engl J Med 368 (2013), 1121–1130.
4. 4 Pant, C., Deshpande, A., Gilroy, R., Olyaee, M., Donskey, C.J., Rising incidence of Clostridium difficile–related discharges among hospitalized children in the United States. Infect Control Hosp Epidemiol 37 (2016), 104–106.
5. 5 Wikswo, M.E., Hall, A.J., Outbreaks of acute gastroenteritis transmitted by person-to-person contact—United States, 2009-2010. MMWR Surveill Summ 61 (2012), 1–12.
6. 6 Buesa, J., Colomina, J., Raga, J., Villanueva, A., Prat, J., Evaluation of reverse transcription and polymerase chain reaction (RT/PCR) for the detection of rotaviruses: applications of the assay. Res Virol 147 (1996), 353–361.
7. 7 Anderson, N.W., Buchan, B.W., Ledeboer, N.A., Comparison of the BD MAX enteric bacterial panel to routine culture methods for detection of Campylobacter, enterohemorrhagic Escherichia coli (O157), Salmonella, and Shigella isolates in preserved stool specimens. J Clin Microbiol 52 (2014), 1222–1224.
8. 8 Knabl, L., Grutsch, I., Orth-Höller, D., Comparison of the BD MAX enteric bacterial panel assay with conventional diagnostic procedures in diarrheal stool samples. Eur J Clin Microbiol Infect Dis 35 (2016), 131–136.
9. 9 Polage, C.R., Gyorke, C.E., Kennedy, M.A., Leslie, J.L., Chin, D.L., Wang, S., et al. Overdiagnosis of Clostridium difficile infection in the molecular test era. JAMA Intern Med 175 (2015), 1792–1801.
10. 10 Liu, J., Gratz, J., Amour, C., Kibiki, G., Becker, S., Janaki, L., et al. A laboratory-developed TaqMan Array Card for simultaneous detection of 19 enteropathogens. J Clin Microbiol 51 (2013), 472–480.
11. 11 Wessels, E., Rusman, L.G., van Bussel, M.J., Claas, E.C., Added value of multiplex Luminex Gastrointestinal Pathogen Panel (xTAG GPP) testing in the diagnosis of infectious gastroenteritis. Clin Microbiol Infect 20 (2014), O182–O187.
12. 12 Siah, S.P., Merif, J., Kaur, K., Nair, J., Huntington, P.G., Karagiannis, T., et al. Improved detection of gastrointestinal pathogens using generalised sample processing and amplification panels. Pathology 46 (2014), 53–59.
13. 13 Onori, M., Coltella, L., Mancinelli, L., Argentieri, M., Menichella, D., Villani, A., et al. Evaluation of a multiplex PCR assay for simultaneous detection of bacterial and viral enteropathogens in stool samples of paediatric patients. Diagn Microbiol Infect Dis 79 (2014), 149–154.
14. 14 Beckmann, C., Heininger, U., Marti, H., Hirsch, H.H., Gastrointestinal pathogens detected by multiplex nucleic acid amplification testing in stools of pediatric patients and patients returning from the tropics. Infection 42 (2014), 961–970.
15. 15 Navidad, J.F., Griswold, D.J., Gradus, M.S., Bhattacharyya, S., Evaluation of Luminex xTAG gastrointestinal pathogen analyte-specific reagents for high-throughput, simultaneous detection of bacteria, viruses, and parasites of clinical and public health importance. J Clin Microbiol 51 (2013), 3018–3024.
16. 16 Payne, D.C., Staat, M.A., Edwards, K.M., Szilagyi, P.G., Gentsch, J.R., Stockman, L.J., et al. Active, population-based surveillance for severe rotavirus gastroenteritis in children in the United States. Pediatrics 122 (2008), 1235–1243.
17. 17 Chhabra, P., Payne, D.C., Szilagyi, P.G., Edwards, K.M., Staat, M.A., Shirley, S.H., et al. Etiology of viral gastroenteritis in children <5 years of age in the United States, 2008-2009. J Infect Dis 208 (2013), 790–800.
18. 18 Aliabadi, N., Tate, J.E., Haynes, A.K., Parashar, U.D., Sustained decrease in laboratory detection of rotavirus after implementation of routine vaccination—United States, 2000-2014. MMWR Morbid Mortal Wkly Rep 64 (2015), 337–342.
19. 19 Denno, D.M., Shaikh, N., Stapp, J.R., Qin, X., Hutter, C.M., Hoffman, V., et al. Diarrhea etiology in a pediatric emergency department: a case-control study. Clin Infect Dis 55 (2012), 897–904.
20. 20 Wendt, J.M., Cohen, J.A., Mu, Y., Dumyati, G.K., Dunn, J.R., Holzbauer, S.M., et al. Clostridium difficile infection among children across diverse US geographic locations. Pediatrics 133 (2014), 651–658.
21. 21 Zilberberg, M.D., Tillotson, G.S., McDonald, C., Clostridium difficile infections among hospitalized children, United States, 1997-2006. Emerg Infect Dis 16 (2010), 604–609.
22. 22 Kim, J., Smathers, S.A., Prasad, P., Leckerman, K.H., Coffin, S., Zaoutis, T., Epidemiological features of Clostridium difficile–associated disease among inpatients at children's hospitals in the United States, 2001-2006. Pediatrics 122 (2008), 1266–1270.
23. 23 Lessa, F.C., Mu, Y., Bamberg, W.M., Beldavs, Z.G., Dumyati, G.K., Dunn, J.R., et al. Burden of Clostridium difficile infection in the United States. N Engl J Med 372 (2015), 825–834.
24. 24 Holst, E., Helin, I., Mårdh, P.A., Recovery of Clostridium difficile from children. Scand J Infect Dis 13 (1981), 41–45.
25. 25 Matsuki, S., Ozaki, E., Shozu, M., Inoue, M., Shimizu, S., Yamaguchi, N., et al. Colonization by Clostridium difficile of neonates in a hospital, and infants and children in three day-care facilities of Kanazawa, Japan. Int Microbiol 8 (2005), 43–48.
26. 26 Jangi, S., Lamont, J.T., Asymptomatic colonization by Clostridium difficile in infants: implications for disease in later life. J Pediatr Gastroenterol Nutr 51 (2010), 2–7.
27. 27 Dominguez, S.R., Dolan, S.A., West, K., Dantes, R.B., Epson, E., Friedman, D., et al. High colonization rate and prolonged shedding of Clostridium difficile in pediatric oncology patients. Clin Infect Dis 59 (2014), 401–403.
28. 28 Hourigan, S.K., Sears, C.L., Oliva-Hemker, M., Clostridium difficile infection in pediatric inflammatory bowel disease. Inflamm Bowel Dis 22 (2016), 1020–1025.
29. 29 Leibowitz, J., Soma, V.L., Rosen, L., Ginocchio, C.C., Rubin, L.G., Similar proportions of stool specimens from hospitalized children with and without diarrhea test positive for Clostridium difficile. Pediatr Infect Dis J 34 (2015), 261–266.
30. 30 El Feghaly, R.E., Stauber, J.L., Tarr, P.I., Haslam, D.B., Viral co-infections are common and are associated with higher bacterial burden in children with Clostridium difficile infection. J Pediatr Gastroenterol Nutr 57 (2013), 813–816.
31. 31 Nicholson, M.R., Thomsen, I.P., Slaughter, J.C., Creech, C.B., Edwards, K.M., Novel risk factors for recurrent Clostridium difficile infection in children. J Pediatr Gastroenterol Nutr 60 (2015), 18–22.
32. 32 Longtin, Y., Trottier, S., Brochu, G., Paquet-Bolduc, B., Garenc, C., Loungnarath, V., et al. Impact of the type of diagnostic assay on Clostridium difficile infection and complication rates in a mandatory reporting program. Clin Infect Dis 56 (2013), 67–73.
33. 33 Koo, H.L., Van, J.N., Zhao, M., Ye, X., Revell, P.A., Jiang, Z.D., et al. Real-time polymerase chain reaction detection of asymptomatic Clostridium difficile colonization and rising C difficile-associated disease rates. Infect Control Hosp Epidemiol 35 (2014), 667–673.
34. 34 Hsu, V.P., Staat, M.A., Roberts, N., Thieman, C., Bernstein, D.I., Bresee, J., et al. Use of active surveillance to validate international classification of diseases code estimates of rotavirus hospitalizations in children. Pediatrics 115 (2005), 78–82.