Transient activation of human cytomegalovirus lytic gene expression during latency allows cytotoxic T cell killing of latently infected cells

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Krishna, B A ^a   Lau, B ^a   Jackson, S E ^a   Wills, M R ^a   Sinclair, J H ^a   Poole, E ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

HISTONE DEACETYLASE INHIBITOR;

COCULTURE; CYTOMEGALOVIRUS; CYTOTOXIC T LYMPHOCYTE; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION REGULATION; GENETICS; HUMAN; IMMUNOLOGY; VIRUS GENE; VIRUS LATENCY;

COCULTURE TECHNIQUES; CYTOMEGALOVIRUS; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION REGULATION, VIRAL; GENES, VIRAL; HISTONE DEACETYLASE INHIBITORS; HUMANS; T-LYMPHOCYTES, CYTOTOXIC; VIRUS LATENCY;

EID: 84973165749     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep24674     Document Type: Article

References (24)

1. Alford, C. A., Stagno, S. & Pass, R. F. Natural history of perinatal cytomegaloviral infection. Ciba Found Symp, 77, 125-147 (1979).
2. Fishman, J. A. Infections in immunocompromised hosts and organ transplant recipients: essentials. Liver Transpl 17 Suppl 3, S34-37 (2013).
3. Khanna, R. & Diamond, D. J. Human cytomegalovirus vaccine: time to look for alternative options. Trends Mol Med 12, 26-33 (2006).
4. Hahn, G., Jores, R. & Mocarski, E. S. Cytomegalovirus remains latent in a common precursor of dendritic and myeloid cells. Proc Natl Acad Sci USA 95, 3937-3942 (1998).
5. Popovic, M. et al. Human cytomegalovirus infection and atherothrombosis. J Thromb Thrombolysis 33, 160-172 (2013).
6. Sinclair, J. & Poole, E. Human cytomegalovirus latency and reactivation in and beyond the myeloid lineage. Future Virology 6, 7 (2014).
7. Reeves, M. B., MacAry, P. A., Lehner, P. J., Sissons, J. G. & Sinclair, J. H. Latency, chromatin remodeling, and reactivation of human cytomegalovirus in the dendritic cells of healthy carriers. Proc Natl Acad Sci USA 102, 4140-4145 (2005).
8. Reeves, M. et al. Autorepression of the human cytomegalovirus major immediate-early promoter/enhancer at late times of infection is mediated by the recruitment of chromatin remodeling enzymes by IE86. J Virol 80, 9998-10009 (2006).
9. Poole, E. et al. Alveolar macrophages isolated directly from HCMV seropositive individuals are sites of HCMV reactivation in vivo. J Infect Dis, doi: 10.1093/infdis/jiu837 (2014).
10. Murphy, J. C., F., W., Verdin, E. & Sinclair, J. H. Control of cytomegalovirus lytic gene expression by histone acetylation. EMBO J. 21, 1112-1120 (2002).
11. Reeves, M. B., Lehner, P. J., Sissons, J. G. & Sinclair, J. H. An in vitro model for the regulation of human cytomegalovirus latency and reactivation in dendritic cells by chromatin remodelling. J Gen Virol 86, 2949-2954 (2005).
12. Poole, E., Wills, M. & Sinclair, J. Human Cytomegalovirus Latency: Targeting Differences in the Latently Infected Cell with a view to Clearing Latent Infection. New Journal of Science 2014, 10 (2014).
13. Mason, G. M., Poole, E., Sissons, J. G., Wills, M. R. & Sinclair, J. H. Human cytomegalovirus latency alters the cellular secretome, inducing cluster of differentiation (CD)4+ T-cell migration and suppression of effector function. Proc Natl Acad Sci USA 109, 14538-14543 (2012).
14. Weekes, M. P. et al. Latency-associated degradation of the MRP1 drug transporter during latent human cytomegalovirus infection. Science 340, 199-202 (2013).
15. Poole, E., McGregor Dallas, S. R., Colston, J., Joseph, R. S. & Sinclair, J. Virally induced changes in cellular microRNAs maintain latency of human cytomegalovirus in CD34 progenitors. J Gen Virol 92, 1539-1549 (2011).
16. Winbanks, C. E. et al. TGF-beta regulates miR-206 and miR-29 to control myogenic differentiation through regulation of HDAC4. J Biol Chem 286, 13805-13814 (2011).
17. Nebbioso, A. et al. Selective class II HDAC inhibitors impair myogenesis by modulating the stability and activity of HDAC-MEF2 complexes. EMBO Rep 10, 776-782 (2009).
18. Travers, H., Spotswood, H. T., Moss, P. A. & Turner, B. M. Human CD34+ hematopoietic progenitor cells hyperacetylate core histones in response to sodium butyrate, but not trichostatin A. Experimental cell research 280, 149-158 (2002).
19. Jackson, S. E., Mason, G. M. & Wills, M. R. Human cytomegalovirus immunity and immune evasion. Virus Res 157, 151-160 (2013).
20. Poole, E., Reeves, M. & Sinclair, J. H. The use of primary human cells (fibroblasts, monocytes, and others) to assess human cytomegalovirus function. Methods Mol Biol 1119, 81-98, doi: 10.1007/978-1-62703-788-4-6 (2014).
21. Poole, E. et al. The myeloid transcription factor GATA-2 regulates the viral UL144 gene during human cytomegalovirus latency in an isolate-specific manner. J Virol 87, 4261-4271 (2013).
22. Poole, E., King, C. A., Sinclair, J. H. & Alcami, A. The UL144 gene product of human cytomegalovirus activates NFkappaB via a TRAF6-dependent mechanism. Embo J 25, 4390-4399 (2006).
23. Straschewski, S. et al. Human cytomegaloviruses expressing yellow fluorescent fusion proteins-characterization and use in antiviral screening. PLoS One 5, e9174 (2010).
24. Poole, E. et al. NF-kappaB-mediated activation of the chemokine CCL22 by the product of the human cytomegalovirus gene UL144 escapes regulation by viral IE86. J Virol 82, 4250-4256 (2008).