High yield expression and purification of Chikungunya virus E2 recombinant protein and its evaluation for serodiagnosis

Journal of Virological Methods

Volumn 235, Issue , 2016, Pages 73-79

  Verma, Anil ^a   Chandele, Anmol ^c   Nayak, Kaustuv ^c   Kaja, Murali Krishna ^c   Arulandu, Arockiasamy ^c   Lodha, Rakesh ^a   Ray, Pratima ^b  

^a ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

^b HAMDARD UNIVERSITY   (India)

^c INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY ICGEB   (India)

Author keywords

Chikungunya; Diagnostics; ELISA; Envelope 2 protein

Indexed keywords

GLYCOPROTEIN E2; IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN M ANTIBODY; RECOMBINANT PROTEIN; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; VIRUS ANTIBODY; VIRUS ANTIGEN; VIRUS ENVELOPE PROTEIN;

ANTIBODY DETECTION; ARTICLE; CHIKUNGUNYA; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SERODIAGNOSIS; STRUCTURE ANALYSIS; WESTERN BLOTTING; AFFINITY CHROMATOGRAPHY; BIOSYNTHESIS; BLOOD; CHEMISTRY; CHIKUNGUNYA FEVER; CHIKUNGUNYA VIRUS; EVALUATION STUDY; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; PROCEDURES; PROTEIN FOLDING; SENSITIVITY AND SPECIFICITY;

ANTIBODIES, VIRAL; ANTIGENS, VIRAL; CHIKUNGUNYA FEVER; CHIKUNGUNYA VIRUS; CHROMATOGRAPHY, AFFINITY; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; PROTEIN FOLDING; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY; VIRAL ENVELOPE PROTEINS;

EID: 84973106707     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2016.05.003     Document Type: Article

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