High efficiency of targeted mutagenesis in arabidopsis via meiotic promoter-driven expression of Cas9 endonuclease

Plant Cell Reports

Volumn 35, Issue 7, 2016, Pages 1555-1558

  Eid, Ayman ^a   Ali, Zahir ^a   Mahfouz, Magdy M ^a  

^a KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Saudi Arabia)

Author keywords

Arabidopsis; CRISPR Cas9; Genome editing; Genome engineering; Meiotic promoters; Targeted mutagenesis

Indexed keywords

ARABIDOPSIS PROTEIN; ENDONUCLEASE;

ARABIDOPSIS; BIOLOGICAL MODEL; CRISPR CAS SYSTEM; GENE EDITING; GENETIC ENGINEERING; GENETICS; HOMOLOGOUS RECOMBINATION; HOMOZYGOTE; MEIOSIS; METABOLISM; MUTATION; NUCLEOTIDE SEQUENCE; PROCEDURES; PROMOTER REGION; REPRODUCIBILITY; SITE DIRECTED MUTAGENESIS; TRANSGENIC PLANT;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; BASE SEQUENCE; CRISPR-CAS SYSTEMS; ENDONUCLEASES; GENE EDITING; GENETIC ENGINEERING; HOMOLOGOUS RECOMBINATION; HOMOZYGOTE; MEIOSIS; MODELS, GENETIC; MUTAGENESIS, SITE-DIRECTED; MUTATION; PLANTS, GENETICALLY MODIFIED; PROMOTER REGIONS, GENETIC; REPRODUCIBILITY OF RESULTS;

EID: 84971006580     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-016-2000-4     Document Type: Article

References (11)

1. Ali Z, Abulfaraj A, Idris A, Ali S, Tashkandi M, Mahfouz MM (2015) CRISPR/Cas9-mediated viral interference in plants. Genome Biol 16:238
2. Aouida M, Piatek MJ, Bangarusamy DK, Mahfouz MM (2014) Activities and specificities of homodimeric TALENs in Saccharomyces cerevisiae. Curr Genet 60:61–74
3. Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743
4. Fauser F, Schiml S, Puchta H (2014) Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. Plant J 79:348–359
5. Feng Z, Mao Y, Xu N, Zhang B, Wei P, Yang DL, Wang Z, Zhang Z, Zheng R, Yang L, Zeng L, Liu X, Zhu JK (2014) Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis. Proc Natl Acad Sci USA 111:4632–4637
6. Li J, Farmer AD, Lindquist IE, Dukowic-Schulze S, Mudge J, Li T, Retzel EF, Chen C (2012) Characterization of a set of novel meiotically-active promoters in Arabidopsis. BMC Plant Biol 12:104
7. Mahfouz MM, Piatek A, Stewart CN Jr (2014) Genome engineering via TALENs and CRISPR/Cas9 systems: challenges and perspectives. Plant Biotechnol J 12:1006–1014
8. Mao Y, Zhang Z, Feng Z, Wei P, Zhang H, Botella JR, Zhu JK (2016) Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications in Arabidopsis. Plant Biotechnol J 14:519–532
9. Wang ZP, Xing HL, Dong L, Zhang HY, Han CY, Wang XC, Chen QJ (2015) Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation. Genome Biol 16:144
10. Yan L, Wei S, Wu Y, Hu R, Li H, Yang W, Xie Q (2015) High-efficiency genome editing in Arabidopsis using YAO promoter-driven CRISPR/Cas9 system. Mol Plant 8:1820–1823
11. Ye GN, Stone D, Pang SZ, Creely W, Gonzalez K, Hinchee M (1999) Arabidopsis ovule is the target for Agrobacterium in planta vacuum infiltration transformation. Plant J 19:249–257