Drug Discovery Goes Three-Dimensional: Goodbye to Flat High-Throughput Screening?

Assay and Drug Development Technologies

Volumn 13, Issue 5, 2015, Pages 262-265

  Eglen, Richard M ^a   Randle, David H ^a  

^a Corning Life Sciences ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR 2; MACROGOL DERIVATIVE; MITOGEN ACTIVATED PROTEIN KINASE; MOLECULAR SCAFFOLD; PHOSPHATIDYLINOSITOL 3 KINASE;

CELL CULTURE; CONFORMATION; DRUG DEVELOPMENT; EX VIVO STUDY; EXTRACELLULAR MATRIX; GEL; HIGH THROUGHPUT SCREENING; HUMAN; HYDROGEL; IN VIVO STUDY; INDUCED PLURIPOTENT STEM CELL; INTERMETHOD COMPARISON; LABORATORY DEVICE; MICROENVIRONMENT; NONHUMAN; REVIEW; SURFACE PROPERTY; THREE DIMENSIONAL CELL CULTURE; TUMOR SPHEROID; TWO DIMENSIONAL CELL CULTURE; CULTURE TECHNIQUE; DRUG SCREENING; MCF 7 CELL LINE; PROCEDURES; TRENDS;

CELL CULTURE TECHNIQUES; DRUG DISCOVERY; DRUG SCREENING ASSAYS, ANTITUMOR; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MCF-7 CELLS;

EID: 84965181368     PISSN: 1540658X     EISSN: 15578127     Source Type: Journal    
DOI: 10.1089/adt.2015.647     Document Type: Review

References (22)

1. Hsu PD, Lander ES, Zhang F: Development and applications of CRISPR-Cas9 for genome engineering. Cell 2014;157:1262-1278.
2. Eglen RM, Reisine T: Primary cells and stem cells in drug discovery: emerging tools for high-throughput screening. Assay Drug Dev Technol 2011;9:108-124.
3. Abbott A: Cell culture: biology's new dimension. Nature 2003;424:870-872.
4. Sanyal S: Culture and Assay Systems Used for 3D Cell Culture, Review Article Corning Life Sciences Application Note 245, Corning Life Sciences. Available at www.corning.com/lifesciences (2014)
5. Edmondson R, Broglie JJ, Adcock AF, Yang L: Three-dimensional cell culture systems and their applications in drug discovery and cell-based sensors. Assay Drug Dev Technol 2014;12:207-218.
6. Kunz-Schughart LA, Freyer JP, Hofstaedter F, Ebner R: The use of 3-d cultures for high-throughput screening: the multicellular spheroid model. J Biomol Screen 2004;9:273-285.
7. Antoni D, Burckel H, Josset E, Noel G: Three-dimensional cell culture: a breakthrough in vivo. Int J Mol Sci 2015;16:5517-5527.
8. Benien P, Swami A: 3D tumor models: history, advances and future perspectives. Future Oncol 2014;10:1311-1327.
9. Inch WR, McCredie JA, Sutherland RM: Growth of nodular carcinomas in rodents compared with multi-cell spheroids in tissue-culture. Growth 1970;34: 271-282.
10. Weiswald LB, Bellet D, Dangles-Marie V: Spherical cancer models in tumor biology. Neoplasia 2015;17:1-15.
11. Shamir ER, Ewald AJ: Three-dimensional organotypic culture: experimental models of mammalian biology and disease. Nat Rev Mol Cell Biol 2014;15:647-664.
12. Pickl M, Reis CH: Comparison of 3D and 2D tumor models reveals enhanced HER2 activation in 3D associated with an increased response to trastuzumab. Oncogene 2009;28:461-468.
13. Vinci M, Gowan S, Boxall F, et al.: Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation. BMC Biol 2012;10:1-20.
14. Yu M, Bardia A, Aceto N, et al.: Ex vivo culture of circulating breast tumor cells for individualized testing of drug susceptibility. Science 2014;345:216-220.
15. Sasai Y: Next-generation regenerative medicine: organogenesis from stem cells in 3D culture. Cell Stem Cell 2013;12:520-530.
16. Adjai IM, Sharma B: Modulation of the tumor microenvironment for cancer treatment: a biomaterials approach. J Funct Biomater 2015;6:81-103.
17. Gelain F, Bottai D, Vescovi A, Zhang S: Designer self-assembling peptide nanofiber scaffolds for adult mouse neural stem cell 3-dimensional cultures. PLoS One 2006;1:e119.
18. Lutolf MP, Gilbert PM, Blau HM: designing materials to direct stem-cell fate. Nature 2009;462:433-441
19. Kong D, Chen M, Gentz R, Zhang J: Cell growth and protein formation on various microcarriers. Cytotechnology 1999;29:149-156.
20. Higuchi A, Qing-Dong L, Kumar SS, et al.: External stimulus-responsive biomaterials designed for the culture and differentiation of ES, iPS and adult stem cells. Prog Polymer Sci 2014;39:1585-1613.
21. Bhatia SN, Ingber DE: Microfluidic organs-on-chips. Nat Biotech 2014;32:760-772.
22. Choi SH, Kim Y, Hebisch M, et al.: A three-dimensional human neural cell culture model of Alzheimer's disease. Nature 2014;515:274-278.