Use of Existing Diagnostic Reverse-Transcription Polymerase Chain Reaction Assays for Detection of Ebola Virus RNA in Semen

Journal of Infectious Diseases

Volumn 213, Issue 8, 2016, Pages 1237-1239

  Pettitt, James ^a   Higgs, Elizabeth S ^a   Adams, Rick D ^a   Jahrling, Peter B ^a   Hensley, Lisa E ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

diagnosis; Ebola; PCR; semen

Indexed keywords

VIRUS DNA;

ARTICLE; CONTROLLED STUDY; EBOLAVIRUS; HUMAN; HUMAN CELL; LIBERIA; MALE; NONHUMAN; NORMAL HUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SEMEN ANALYSIS; SEMINAL PLASMA; SENSITIVITY ANALYSIS; SEXUAL TRANSMISSION; VIRUS DETECTION; VIRUS TITRATION; VIRUS TRANSMISSION; GENETICS; HEMORRHAGIC FEVER, EBOLA; PROCEDURES; SPERM; VIROLOGY;

EBOLAVIRUS; HEMORRHAGIC FEVER, EBOLA; HUMANS; MALE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEMEN; VIROLOGY;

EID: 84965174611     PISSN: 00221899     EISSN: 15376613     Source Type: Journal    
DOI: 10.1093/infdis/jiv454     Document Type: Article

References (7)

1. Christie A, Davies-Wayne GJ, Cordier-Lasalle T, et al. Possible sexual transmission of Ebola virus-Liberia, 2015. MMWR Morb Mortal Wkly Rep 2015; 64:479-81.
2. Bausch DG, Towner JS, Dowell SF, et al. Assessment of the risk of Ebola virus transmission from bodily fluids and fomites. J Infect Dis 2007; 196:S142-7.
3. Rogstad KE, Tunbridge A. Ebola virus as a sexually transmitted infection. Curr OpinInfect Dis 2015; 28:83-5.
4. World Health Organization. Interim advice on the sexual transmission of the Ebola virus disease. 8 May 2015. http://www.who.int/reproductivehealth/topics/rtis/ebola-virus-semen/en/. Accessed 12 September 2015.
5. JVAP-MCS. Ebola Zaire (EZ1) rRT-PCR (TaqMan) assay on ABI 7500 Fast Dx, Lightcycler, and JBAIDS: instruction booklet. http://www.fda.gov/downloads/MedicalDevices/Safety/EmergencySituations/UCM408334.pdf. Accessed 23 July 2015.
6. Trombley AR, Wachter L, Garrison J, et al. Comprehensive panel of real-time TaqMan polymerase chain reaction assays for detection and absolute quantification of filoviruses, arenaviruses, and New World hantaviruses. Am J Trop Med Hyg 2010; 82:954-60.
7. Sozhamannan S, Holland MY, Hall AT, et al. Evaluation of signature erosion in Ebola virus due to genomic drift and its impact on the performance of diagnostic assays. Viruses 2015; 7:3130-54.