Homogeneity of the Vaginal Microbiome at the Cervix, Posterior Fornix, and Vaginal Canal in Pregnant Chinese Women

Microbial Ecology

Volumn 69, Issue 2, 2015, Pages 407-414

  Huang, Yi E ^a,b   Wang, Yan ^a   He, Yan ^a   Ji, Yong ^a   Wang, Li Ping ^a   Sheng, Hua Fang ^a   Zhang, Min ^a   Huang, Qi Tao ^a   Zhang, Dong Jing ^a   Wu, Jing Jing ^a   Zhong, Mei ^a   Zhou, Hong Wei ^a  

^a SOUTHERN MEDICAL UNIVERSITY   (China)

^b CENTRAL SOUTH UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; RNA 16S;

ADULT; ASIAN CONTINENTAL ANCESTRY GROUP; BACTERIUM; CLASSIFICATION; FEMALE; GENETICS; HUMAN; ISOLATION AND PURIFICATION; LACTOBACILLUS; MICROBIOLOGY; MICROFLORA; PREGNANCY; UTERINE CERVIX; VAGINA; VAGINITIS; YOUNG ADULT;

ADULT; ASIAN CONTINENTAL ANCESTRY GROUP; BACTERIA; CERVIX UTERI; CLASSIFICATION; DNA, BACTERIAL; FEMALE; GENETICS; HUMANS; ISOLATION AND PURIFICATION; LACTOBACILLUS; MICROBIOLOGY; MICROBIOTA; PREGNANCY; RNA, RIBOSOMAL, 16S; VAGINA; VAGINOSIS, BACTERIAL; YOUNG ADULT;

BACTERIA (MICROORGANISMS); LACTOBACILLUS; LACTOBACILLUS CRISPATUS; LACTOBACILLUS GASSERI; LACTOBACILLUS INERS;

EID: 84964237803     PISSN: 00953628     EISSN: 1432184X     Source Type: Journal    
DOI: 10.1007/s00248-014-0487-1     Document Type: Article

References (35)

1. Verstraelen H, Verhelst R, Claeys G, De Backer E, Temmerman M, Vaneechoutte M (2009) Longitudinal analysis of the vaginal microflora in pregnancy suggests that L. crispatus promotes the stability of the normal vaginal microflora and that L. gasseri and/or L. iners are more conducive to the occurrence of abnormal vaginal microflora. BMC Microbiol 116
2. Hernandez-Rodriguez C, Romero-Gonzalez R, Albani-Campanario M, Figueroa-Damian R, Meraz-Cruz N, Hernandez-Guerrero C (2011) Vaginal microbiota of healthy pregnant Mexican women is constituted by four Lactobacillus species and several vaginosis-associated bacteria. Infect Dis Obstet Gynecol 2011:851485
3. Verhelst R, Verstraelen H, Claeys G, Verschraegen G, Van Simaey L, De Ganck C, De Backer E, Temmerman M, Vaneechoutte M (2005) Comparison between Gram stain and culture for the characterization of vaginal microflora: definition of a distinct grade that resembles grade I microflora and revised categorization of grade I microflora. BMC Microbiol 5:61
4. Hyman RW, Herndon CN, Jiang H, Palm C, Fukushima M, Bernstein D, Vo KC, Zelenko Z, Davis RW, Giudice LC (2012) The dynamics of the vaginal microbiome during infertility therapy with in vitro fertilization-embryo transfer. J Assist Reprod Genet 29:105–115
5. Boskey ER, Cone RA, Whaley KJ, Moench TR (2001) Origins of vaginal acidity: high D/L lactate ratio is consistent with bacteria being the primary source. Hum Reprod 16:1809–1813
6. Donders GG, Van Calsteren K, Bellen G, Reybrouck R, Van den Bosch T, Riphagen I, Van Lierde S (2009) Predictive value for preterm birth of abnormal vaginal flora, bacterial vaginosis and aerobic vaginitis during the first trimester of pregnancy. BJOG 116:1315–1324
7. Hillier SL, Nugent RP, Eschenbach DA, Krohn MA, Gibbs RS, Martin DH, Cotch MF, Edelman R, Pastorek JG 2nd, Rao AV et al (1995) Association between bacterial vaginosis and preterm delivery of a low-birth-weight infant. The vaginal infections and prematurity study group. N Engl J Med 333:1737–1742
8. Leitich H, Bodner-Adler B, Brunbauer M, Kaider A, Egarter C, Husslein P (2003) Bacterial vaginosis as a risk factor for preterm delivery: a meta-analysis. Am J Obstet Gynecol 189:139–147
9. Gajer P, Brotman RM, Bai G, Sakamoto J, Schutte UM, Zhong X, Koenig SS, Fu L, Ma ZS, Zhou X, Abdo Z, Forney LJ, Ravel J (2012) Temporal dynamics of the human vaginal microbiota. Sci Transl Med 4:132ra152
10. Aagaard K, Riehle K, Ma J, Segata N, Mistretta TA, Coarfa C, Raza S, Rosenbaum S, Van den Veyver I, Milosavljevic A, Gevers D, Huttenhower C, Petrosino J, Versalovic J (2012) A metagenomic approach to characterization of the vaginal microbiome signature in pregnancy. PLoS ONE 7:e36466
11. Romero R, Hassan SS, Gajer P, Tarca AL, Fadrosh DW, Nikita L, Galuppi M, Lamont RF, Chaemsaithong P, Miranda J, Chaiworapongsa T, Ravel J (2014) The composition and stability of the vaginal microbiota of normal pregnant women is different from that of non-pregnant women. Microbiome 2:4
12. Cole AM (2006) Innate host defense of human vaginal and cervical mucosae. Curr Top Microbiol Immunol 306:199–230
13. Bartlett JG, Moon NE, Goldstein PR, Goren B, Onderdonk AB, Polk BF (1978) Cervical and vaginal bacterial flora: ecologic niches in the female lower genital tract. Am J Obstet Gynecol 130:658–661
14. Bartlett JG, Polk BF (1984) Bacterial flora of the vagina: quantitative study. Rev Infect Dis 6(Suppl 1):S67–S72
15. Ling Z, Liu X, Chen X, Zhu H, Nelson KE, Xia Y, Li L, Xiang C (2011) Diversity of cervico vaginal microbiota associated with female lower genital tract infections. Microb Ecol 61:704–714
16. Kim TK, Thomas SM, Ho M, Sharma S, Reich CI, Frank JA, Yeater KM, Biggs DR, Nakamura N, Stumpf R, Leigh SR, Tapping RI, Blanke SR, Slauch JM, Gaskins HR, Weisbaum JS, Olsen GJ, Hoyer LL, Wilson BA (2009) Heterogeneity of vaginal microbial communities within individuals. J Clin Microbiol 47:1181–1189
17. Forney LJ, Gajer P, Williams CJ, Schneider GM, Koenig SS, McCulle SL, Karlebach S, Brotman RM, Davis CC, Ault K, Ravel J (2010) Comparison of self-collected and physician-collected vaginal swabs for microbiome analysis. J Clin Microbiol 48:1741–1748
18. Zhou HW, Li DF, Tam NF, Jiang XT, Zhang H, Sheng HF, Qin J, Liu X, Zou F (2011) BIPES, a cost-effective high-throughput method for assessing microbial diversity. ISME J 5:741–749
19. Peng X, Yu KQ, Deng GH, Jiang YX, Wang Y, Zhang GX et al. (2013) Comparison of direct boiling method with commercial kits for extracting fecal microbiome DNA by Illumina sequencing of 16S rRNA tags. J Microbiol Methods
20. Edgar RC, Haas BJ, Clemente JC, Quince C, Knight R (2011) UCHIME improves sensitivity and speed of chimera detection. Bioinformatics 27:2194–2200
21. Wang Q, Garrity GM, Tiedje JM, Cole JR (2007) Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 73:5261–5267
22. Lozupone C, Lladser ME, Knights D, Stombaugh J, Knight R (2011) UniFrac: an effective distance metric for microbial community comparison. ISME J 5:169–172
23. LozuponeC KR (2005) UniFrac: a new phylogenetic method for comparing microbial communities. Appl Environ Microbiol 71:8228–8235
24. SPSS for Windows, Rel. 17.0.1. (2009). Chicago: SPSS Inc.
25. Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett WS, Huttenhower C (2011) Metagenomic biomarker discovery and explanation. Genome Biol 12:R60
26. Zhou X, Brown CJ, Abdo Z, Davis CC, Hansmann MA, Joyce P, Foster JA, Forney LJ (2007) Differences in the composition of vaginal microbial communities found in healthy Caucasian and black women. ISME J 1:121–133
27. Kiss H, Kogler B, Petricevic L, Sauerzapf I, Klayraung S, Domig K, Viernstein H, Kneifel W (2007) Vaginal Lactobacillus microbiota of healthy women in the late first trimester of pregnancy. BJOG 114:1402–1407
28. Bezirtzoglou E, Voidarou C, Papadaki A, Tsiotsias A, Kotsovolou O, Konstandi M (2008) Hormone therapy alters the composition of the vaginal microflora in ovariectomized rats. Microb Ecol 55:751–759
29. Ngai FW, Ngu SF (2014) Predictors of family and marital functioning at early postpartum. J Adv Nurs
30. Kalra A, Palcu CT, Sobel JD, Akins RA (2007) Bacterial vaginosis: culture- and PCR-based characterizations of a complex polymicrobial disease’s pathobiology. Curr Infect Dis Rep 9:485–500
31. Ferris MJ, Norori J, Zozaya-Hinchliffe M, Martin DH (2007) Cultivation-independent analysis of changes in bacterial vaginosis flora following metronidazole treatment. J Clin Microbiol 45:1016–1018
32. Jakobsson T, Forsum U (2007) Lactobacillus iners: a marker of changes in the vaginal flora? J Clin Microbiol 45:3145
33. Fashemi B, Delaney ML, Onderdonk AB, Fichorova RN (2013) Effects of feminine hygiene products on the vaginal mucosal biome. Microbiol Ecol Health Dis 24
34. Gupta S, Kumar N, Singhal N, Kaur R, Manektala U (2006) Vaginal microflora in postmenopausal women on hormone replacement therapy. Indian J Pathol Microbiol 49:457–461
35. Gondo F, da Silva MG, Polettini J, Tristao Ada R, Peracoli JC, Witkin SS, Rudge MV (2011) Vaginal flora alterations and clinical symptoms in low-risk pregnant women. Gynecol Obstet Investig 71:158–162