Revealing Assembly of a Pore-Forming Complex Using Single-Cell Kinetic Analysis and Modeling

Biophysical Journal

Volumn 110, Issue 7, 2016, Pages 1574-1581

  Bischofberger, Mirko ^a   Iacovache, Ioan ^a   Boss, Daniel ^a   Naef, Felix ^a   Van Der Goot, F Gisou ^a   Molina, Nacho ^b  

^a EPFL   (Switzerland)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AEROLYSIN; BACTERIAL TOXIN; PEPTIDE FRAGMENT; PORE FORMING CYTOTOXIC PROTEIN;

CHEMISTRY; CYTOLOGY; ERYTHROCYTE; HUMAN; KINETICS; METABOLISM; MOLECULAR MODEL; PROCEDURES; SINGLE CELL ANALYSIS;

BACTERIAL TOXINS; ERYTHROCYTES; HUMANS; KINETICS; MODELS, MOLECULAR; PEPTIDE FRAGMENTS; PORE FORMING CYTOTOXIC PROTEINS; SINGLE-CELL ANALYSIS;

EID: 84963631892     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2016.02.035     Document Type: Article

References (27)

1. A.P. Minton Implications of macromolecular crowding for protein assembly Curr. Opin. Struct. Biol. 10 2000 34 39
2. A.M. Morris, M.A. Watzky, and R.G. Finke Protein aggregation kinetics, mechanism, and curve-fitting: a review of the literature Biochim. Biophys. Acta 1794 2009 375 397
3. E. Karsenti, F. Nédélec, and T. Surrey Modelling microtubule patterns Nat. Cell Biol. 8 2006 1204 1211
4. M. Bischofberger, M.R. Gonzalez, and F.G. van der Goot Membrane injury by pore-forming proteins Curr. Opin. Cell Biol. 21 2009 589 595
5. I. Iacovache, F.G. van der Goot, and L. Pernot Pore formation: an ancient yet complex form of attack Biochim. Biophys. Acta 1778 2008 1611 1623
6. D.L. Floyd, and J.R. Ragains A.M. van Oijen Single-particle kinetics of influenza virus membrane fusion Proc. Natl. Acad. Sci. USA 105 2008 15382 15387
7. F.G. van der Goot, J.H. Lakey, and F. Pattus The molten globule intermediate for protein insertion or translocation through membranes Trends Cell Biol. 2 1992 343 348
8. L. Abrami, M. Fivaz, and F.G. van der Goot Adventures of a pore-forming toxin at the target cell surface Trends Microbiol. 8 2000 168 172
9. J.T. Buckley Purification of cloned proaerolysin released by a low protease mutant of Aeromonas salmonicida Biochem. Cell Biol. 68 1990 221 224
10. A.P. Heuck, and E.M. Hotze A.E. Johnson Mechanism of membrane insertion of a multimeric β-barrel protein: perfringolysin O creates a pore using ordered and coupled conformational changes Mol. Cell 6 2000 1233 1242
11. I. Iacovache, and M.T. Degiacomi F.G. van der Goot Dual chaperone role of the C-terminal propeptide in folding and oligomerization of the pore-forming toxin aerolysin PLoS Pathog. 7 2011 e1002135
12. Y. Tsitrin, and C.J. Morton F.G. van der Goot Conversion of a transmembrane to a water-soluble protein complex by a single point mutation Nat. Struct. Biol. 9 2002 729 733
13. P. Marquet, and B. Rappaz C. Depeursinge Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy Opt. Lett. 30 2005 468 470
14. R.E. Kass, and A.E. Raftery Bayes factors J. Am. Stat. Assoc. 90 1995 773 795
15. F. van der Goot Pore-Forming Toxins 2001 Springer Berlin, Germany
16. J.T. Buckley, and H.U. Wilmsen F.G. van der Goot Protonation of histidine-132 promotes oligomerization of the channel-forming toxin aerolysin Biochemistry 34 1995 16450 16455
17. 2+ release from intracellular stores in human granulocytes J. Biol. Chem. 273 1998 18122 18129
18. M. Tschödrich-Rotter, and U. Kubitscheck R. Peters Optical single-channel analysis of the aerolysin pore in erythrocyte membranes Biophys. J. 70 1996 723 732
19. C.R. MacKenzie, T. Hirama, and J.T. Buckley Analysis of receptor binding by the channel-forming toxin aerolysin using surface plasmon resonance J. Biol. Chem. 274 1999 22604 22609
20. L. Abrami, and F.G. van Der Goot Plasma membrane microdomains act as concentration platforms to facilitate intoxication by aerolysin J. Cell Biol. 147 1999 175 184
21. F.G. van der Goot, and K.R. Hardie J.T. Buckley The C-terminal peptide produced upon proteolytic activation of the cytolytic toxin aerolysin is not involved in channel formation J. Biol. Chem. 269 1994 30496 30501
22. M.T. Degiacomi, and I. Iacovache M. Dal Peraro Molecular assembly of the aerolysin pore reveals a swirling membrane-insertion mechanism Nat. Chem. Biol. 9 2013 623 629
23. B. Walker, and O. Braha H. Bayley An intermediate in the assembly of a pore-forming protein trapped with a genetically engineered switch Chem. Biol. 2 1995 99 105
24. J.R. Thompson, and B. Cronin M.I. Wallace Rapid assembly of a multimeric membrane protein pore Biophys. J. 101 2011 2679 2683
25. T.K. Sato, R.K. Tweten, and A.E. Johnson Disulfide-bond scanning reveals assembly state and β-strand tilt angle of the PFO β-barrel Nat. Chem. Biol. 9 2013 383 389
26. S. Benke, and D. Roderer B. Schuler The assembly dynamics of the cytolytic pore toxin ClyA Nat. Commun. 6 2015 6198
27. V.T. Nguyen, Y. Kamio, and H. Higuchi Single-molecule imaging of cooperative assembly of γ-hemolysin on erythrocyte membranes EMBO J 22 2003 4968 4979