Genetic engineering of Synechocystis PCC6803 for the photoautotrophic production of the sweetener erythritol

Microbial Cell Factories

Volumn 15, Issue 1, 2016, Pages

  van der Woude, Aniek D ^a   Perez Gallego, Ruth ^a   Vreugdenhil, Angie ^a   Puthan Veetil, Vinod ^a   Chroumpi, Tania ^a   Hellingwerf, Klaas J ^a  

^a Photanol BV   (Netherlands)

Author keywords

Calvin cycle; Cyanobacteria; Erythritol; Erythrose; Erythrose 4 phosphate; Metabolic engineering; Sweetener

Indexed keywords

CARBON DIOXIDE; ERYTHRITOL; ERYTHROSE 4 PHOSPHATE; ERYTHROSE 4 PHOSPHATE PHOSPHATASE; ERYTHROSE REDUCTASE; OXIDOREDUCTASE; PHOSPHATASE; UNCLASSIFIED DRUG; ALDEHYDE REDUCTASE; ERYTHROSE 4-PHOSPHATE; SUGAR PHOSPHATE; SWEETENING AGENT;

ARTICLE; BACTERIAL METABOLISM; BACTERIAL STRAIN; BIOTECHNOLOGY; CARBON DIOXIDE FIXATION; CODON; CONTROLLED STUDY; EXTRACELLULAR SPACE; GENE EXPRESSION; GENETIC ENGINEERING; LIGHT; MOLECULAR CLONING; NONHUMAN; SYNECHOCYSTIS; AUTOTROPHY; BIOSYNTHESIS; ESCHERICHIA COLI; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; PHOTOSYNTHESIS; PROCEDURES; TRANSGENIC ORGANISM;

ALDEHYDE REDUCTASE; AUTOTROPHIC PROCESSES; ERYTHRITOL; ESCHERICHIA COLI; GENETIC ENGINEERING; LIGHT; ORGANISMS, GENETICALLY MODIFIED; PHOTOSYNTHESIS; SUGAR PHOSPHATES; SWEETENING AGENTS; SYNECHOCYSTIS;

EID: 84962881217     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-016-0458-y     Document Type: Article

References (35)

1. Kawanabe J, Hirasawa M, Takeuchi T, Oda T, Ikeda T. Noncariogenicity of erythritol as a substrate. Caries Res. 1992;26:358-62.
2. Moon H-J, Jeya M, Kim I-W, Lee J-K. Biotechnological production of erythritol and its applications. Appl Microbiol Biotechnol. 2010;86:1017-25.
3. von Rymon Lipinski G-W. Sweeteners. Adv Biochem Eng Biotechnol. 2014;143:1-28.
4. Ortiz ME, Bleckwedel J, Raya RR, Mozzi F. Biotechnological and in situ food production of polyols by lactic acid bacteria. Appl Microbiol Biotechnol. 2013;97:4713-26.
5. Jeya M, Lee K-M, Tiwari MK, Kim J-S, Gunasekaran P, Kim S-Y, et al. Isolation of a novel high erythritol-producing Pseudozyma tsukubaensis and scale-up of erythritol fermentation to industrial level. Appl Microbiol Biotechnol. 2009;83:225-31.
6. Ducat DC, Way JC, Silver PA. Engineering cyanobacteria to generate high-value products. Trends Biotechnol. 2011;29:95-103.
7. Oliver JWK, Atsumi S. Metabolic design for cyanobacterial chemical synthesis. Photosynth Res. 2014;120:249-61.
8. Angermayr SA, Gorchs Rovira A, Hellingwerf KJ. Metabolic engineering of cyanobacteria for the synthesis of commodity products. Trends Biotechnol. 2015;33:352-61.
9. Ookura T, Azuma K, Isshiki K, Taniguchi H, Kasumi T, Kawamura Y. Primary structure analysis and functional expression of erythrose reductases from erythritol-producing fungi (Trichosporonoides megachiliensis SNG-42). Biosci Biotechnol Biochem. 2005;69:944-51.
10. Ookura T, Kasumi T. Yeast Gcy1p reduces erythrose and erythrose-4-phosphate. Rep Natl Food Res Inst. 2007;71:57-60.
11. Lee D-H, Lee Y-J, Ryu Y-W, Seo J-H. Molecular cloning and biochemical characterization of a novel erythrose reductase from Candida magnoliae JH110. Microb. Cell Factories. 2010;9:43.
12. Jovanović B, Mach RL, Mach-Aigner AR. Characterization of erythrose reductases from filamentous fungi. AMB Express. 2013;3:43.
13. Takahashi H, Uchimiya H, Hihara Y. Difference in metabolite levels between photoautotrophic and photomixotrophic cultures of Synechocystis sp. PCC 6803 examined by capillary electrophoresis electrospray ionization mass spectrometry. J Exp Bot. 2008;59:3009-18.
14. Richter H, Vlad D, Unden G. Significance of pantothenate for glucose fermentation by Oenococcus oeni and for suppression of the erythritol and acetate production. Arch Microbiol. 2001;175:26-31.
15. Veiga-da-Cunha M, Santos H, Van Schaftingen E. Pathway and regulation of erythritol formation in Leuconostoc oenos. J Bacteriol. 1993;175:3941-8.
16. Angermayr SA, Paszota M, Hellingwerf KJ. Engineering a cyanobacterial cell factory for production of lactic acid. Appl Environ Microbiol. 2012;78:7098-106.
17. Savakis PE, Angermayr SA, Hellingwerf KJ. Synthesis of 2,3-butanediol by Synechocystis sp. PCC6803 via heterologous expression of a catabolic pathway from lactic acid- and enterobacteria. Metab Eng. 2013;20:121-30.
18. Puigbò P, Guzmán E, Romeu A, Garcia-Vallvé S. OPTIMIZER: a web server for optimizing the codon usage of DNA sequences. Nucleic Acids Res. 2007;35:W126-31.
19. Chen Q, van der Steen JB, Dekker HL, Ganapathy S, de Grip WJ, Hellingwerf KJ. Expression of holoproteorhodopsin in Synechocystis sp. PCC 6803. Metab Eng. 2016;35:83-94.
20. Guerrero F, Carbonell V, Cossu M, Correddu D, Jones PR. Ethylene synthesis and regulated expression of recombinant protein in Synechocystis sp. PCC 6803. PLoS ONE. 2012;7:e50470.
21. Xu Y, Alvey RM, Byrne PO, Graham JE, Shen G, Bryant DA. Expression of genes in cyanobacteria: adaptation of endogenous plasmids as platforms for high-level gene expression in Synechococcus sp. PCC 7002. Methods Mol Biol Clifton NJ. 2011;684:273-93.
22. Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepes J, Brahamsha B, et al. Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria. Nucleic Acids Res. 2014;42:e136.
23. van der Woude AD, Angermayr SA, Veetil VP, Osnato A, Hellingwerf KJ. Carbon sink removal: increased photosynthetic production of lactic acid by Synechocystis sp. PCC6803 in a glycogen storage mutant. J Biotechnol. 2014;184C:100-2.
24. Du W, Liang F, Duan Y, Tan X, Lu X. Exploring the photosynthetic production capacity of sucrose by cyanobacteria. Metab Eng. 2013;19:17-25.
25. Lee J-K, Hong K-W, Kim S-Y. Purification and properties of a NADPH-dependent erythrose reductase from the newly isolated Torula corallina. Biotechnol Prog. 2003;19:495-500.
26. Marin K, Stirnberg M, Eisenhut M, Krämer R, Hagemann M. Osmotic stress in Synechocystis sp. PCC 6803: low tolerance towards nonionic osmotic stress results from lacking activation of glucosylglycerol accumulation. Microbiology. 2006;152:2023-30.
27. Kuznetsova E, Proudfoot M, Sanders SA, Reinking J, Savchenko A, Arrowsmith CH, et al. Enzyme genomics: application of general enzymatic screens to discover new enzymes. FEMS Microbiol Rev. 2005;29:263-79.
28. Angermayr SA, Woude AD van der, Correddu D, Vreugdenhil A, Verrone V, Hellingwerf KJ. Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803. Biotechnol Biofuels. 2014;7:99.
29. Kuznetsova E, Proudfoot M, Gonzalez CF, Brown G, Omelchenko MV, Borozan I, et al. Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenase-like phosphatase family. J Biol Chem. 2006;281:36149-61.
30. Nakahara K, Yamamoto H, Miyake C, Yokota A. Purification and Characterization of Class-I and Class-II Fructose-1,6-bisphosphate Aldolases from the Cyanobacterium Synechocystis sp. PCC 6803. Plant Cell Physiol. 2003;44:326-33.
31. 2 in a cyanobacterium. Metab Eng. 2014;21:60-70.
32. Savakis P, Tan X, Du W, Branco dos Santos F, Lu X, Hellingwerf KJ. Photosynthetic production of glycerol by a recombinant cyanobacterium. J Biotechnol. 2015;195:46-51.
33. Dempo Y, Ohta E, Nakayama Y, Bamba T, Fukusaki E. Molar-based targeted metabolic profiling of cyanobacterial strains with potential for biological production. Metabolites. 2014;4:499-516.
34. Carrieri D, Paddock T, Maness P-C, Seibert M, Yu J. Photo-catalytic conversion of carbon dioxide to organic acids by a recombinant cyanobacterium incapable of glycogen storage. Energy Environ Sci. 2012;5:9457-61.
35. Miao X, Wu Q, Wu G, Zhao N. Sucrose accumulation in salt-stressed cells of agp gene deletion-mutant in cyanobacterium Synechocystis sp PCC 6803. FEMS Microbiol Lett. 2003;218:71-7.