Depletion of human DNA in spiked clinical specimens for improvement of sensitivity of pathogen detection by next-generation sequencing

Journal of Clinical Microbiology

Volumn 54, Issue 4, 2016, Pages 919-927

  Hasan, Mohammad R ^a,b   Rawat, Arun ^a   Tang, Patrick ^a   Jithesh, Puthen V ^a   Thomas, Eva ^a   Tan, Rusung ^a,c   Tilley, Peter ^c,d  

^a SIDRA MEDICAL AND RESEARCH CENTER   (Qatar)

^b WEILL CORNELL MEDICAL COLLEGE   (Qatar)

^c PROVINCIAL HEALTH SERVICES AUTHORITY   (Canada)

^d UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; DNA; SAPONIN; VIRUS DNA;

ARTICLE; CEREBROSPINAL FLUID ANALYSIS; CLINICAL PROTOCOL; CONTROLLED STUDY; DNA DETERMINATION; DNA EXTRACTION; DNA SEQUENCE; GENOME ANALYSIS; HUMAN; METAGENOMICS; NEXT GENERATION SEQUENCING; NONHUMAN; PRIORITY JOURNAL; PROCESS DEVELOPMENT; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; CEREBROSPINAL FLUID; COMMUNICABLE DISEASES; EVALUATION STUDY; HIGH THROUGHPUT SEQUENCING; MICROBIOLOGY; MOLECULAR DIAGNOSIS; NASOPHARYNX; PROCEDURES; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; VIROLOGY;

CEREBROSPINAL FLUID; COMMUNICABLE DISEASES; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; NASOPHARYNX; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING;

EID: 84962595445     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.03050-15     Document Type: Article

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