FoCuS-point: Software for STED fluorescence correlation and time-gated single photon counting

Bioinformatics

Volumn 32, Issue 6, 2016, Pages 958-960

  Waithe, Dominic ^a   Clausen, Mathias P ^b   Sezgin, Erdinc ^b   Eggeling, Christian ^a,b  

^a Wolfson Imaging Centre   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ALGORITHM; DIFFUSION; MOLECULAR DYNAMICS; SOFTWARE; SPECTROFLUOROMETRY;

ALGORITHMS; DIFFUSION; MOLECULAR DYNAMICS SIMULATION; SOFTWARE; SPECTROMETRY, FLUORESCENCE;

EID: 84962177645     PISSN: 13674803     EISSN: 14602059     Source Type: Journal    
DOI: 10.1093/bioinformatics/btv687     Document Type: Article

References (15)

1. Clausen,M.P. et al. (2015) A straightforward approach for gated sted-fcs to investigate lipid membrane dynamics. Methods, 88, 67-75.
2. Eggeling,C. et al. (2009) Direct observation of the nanoscale dynamics of membrane lipids in a living cell. Nature, 457, 1159-1162.
3. Ehrenberg,M. and Rigler,R. (1974) Rotational Brownian motion and fluorescence intensify fluctuations. Chem. Phys., 4, 390-401.
4. Enderlein,J. and Gregor,I. (2005) Using fluorescence lifetime for discriminating detector afterpulsing in fluorescence-correlation spectroscopy. Rev. Sci. Instrum., 76, 033102.
5. Hell,S.W. and Wichmann,J. (1994) Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy. Opt. Lett., 19, 780-782.
6. Kastrup,L. et al. (2005) Fluorescence fluctuation spectroscopy in subdiffraction focal volumes. Phys. Rev. Lett., 94, 178104.
7. Magde,D. et al. (1972) Thermodynamic fluctuations in a reacting system-measurement by fluorescence correlation spectroscopy.Phys. Rev. Lett., 29, 705.
8. Moffitt,J.R. et al. (2011) Time-gating improves the spatial resolution of sted microscopy. Opt. Express, 19, 4242-4254.
9. Müller,P. et al. (2014) Pycorrfit-generic data evaluation for fluorescence correlation spectroscopy. Bioinformatics, btu328.
10. Schwille,P. et al. (1999) Fluorescence correlation spectroscopy with single-molecule sensitivity on cell and model membranes. Cytometry, 36, 176-182.
11. Vicidomini,G. et al. (2011) Sharper low-power sted nanoscopy by time gating. Nat. Methods, 8, 571-573.
12. Vicidomini,G. et al. (2015) Sted-flcs: An advanced tool to reveal spatiotemporal heterogeneity of molecular membrane dynamics. Nano Lett., 15, 5912-5918.
13. Wachsmuth,M. et al. (2015) High-throughput fluorescence correlation spectroscopy enables analysis of proteome dynamics in living cells. Nat. Biotechnol., 33, 384-389.
14. Wahl,M. et al. (2003) Fast calculation of fluorescence correlation data with asynchronous time-correlated single-photon counting. Opt. Express, 11, 3583-3591.
15. Widengren,J. and Rigler,R. (1998) Fluorescence correlation spectroscopy as a tool to investigate chemical reactions in solutions and on cell surfaces. Cell. Mol. Biol. (Noisy-le-Grand, France), 44, 857-879.