Comparing apples and oranges: Considerations for quantifying candidal biofilms with XTT [2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide] and the need for standardized testing

Journal of Medical Microbiology

Volumn 65, Issue 4, 2016, Pages 259-260

  Ramage, Gordon ^a  

^a GLASGOW DENTAL HOSPITAL AND SCHOOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CRYSTAL VIOLET; 2,3-BIS (2-METHYLOXY-4-NITRO-5-SULFOPHENYL)-2H-TETRAZOLIUM-5-CARBOXANILIDE; ANILIDE; DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; TETRAZOLIUM;

ANTIFUNGAL SUSCEPTIBILITY; APPLE; BIOASSAY; BIOFILM; CANDIDA; COLORIMETRY; EDITORIAL; FUNGAL BIOMASS; HIGH THROUGHPUT SCREENING; NONHUMAN; ORANGE (FRUIT); PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; STANDARDIZATION; XTT ASSAY; GROWTH, DEVELOPMENT AND AGING; HUMAN; MICROBIOLOGICAL EXAMINATION; PHYSIOLOGY; PROCEDURES; STANDARDS;

ANILIDES; BIOFILMS; CANDIDA; HUMANS; INDICATORS AND REAGENTS; MICROBIOLOGICAL TECHNIQUES; TETRAZOLIUM SALTS;

EID: 84962026439     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.000237     Document Type: Editorial

References (15)

1. Dhale, R. P., Ghorpade, M. V. & Dharmadhikari, C. A. (2014). Comparison of various methods used to detect biofilm production of Candida species. J Clin Diagn Res 8, 18-20.
2. Hawser, S. (1996a). Adhesion of different Candida spp. to plastic: XTT formazan determinations. J Med Vet Mycol 34, 407-410.
3. Hawser, S. (1996b). Comparisons of the susceptibilities of planktonic and adherent Candida albicans to antifungal agents: a modified XTT tetrazolium assay using synchronised C. albicans cells. J Med Vet Mycol 34, 149-152.
4. Hawser, S. P., Norris, H., Jessup, C. J. & Ghannoum, M. A. (1998). Comparison of a 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) colorimetric method with the standardized National Committee for Clinical Laboratory Standards method of testing clinical yeast isolates for susceptibility to antifungal agents. J Clin Microbiol 36, 1450-1452.
5. Kuhn, D. M., Balkis, M., Chandra, J., Mukherjee, P. K. & Ghannoum, M. A. (2003). Uses and limitations of the XTT assay in studies of Candida growth and metabolism. J Clin Microbiol 41, 506-508.
6. Marcos-Zambrano, L. J., Escribano, P., Bouza, E. & Guinea, J. (2014). Production of biofilm by Candida and non-Candida spp. isolates causing fungemia: comparison of biomass production and metabolic activity and development of cut-off points. Int J Med Microbiol 304, 1192-1198.
7. Pierce, C. G., Uppuluri, P., Tristan, A. R., Wormley, F. L., Jr, Mowat, E., Ramage, G. & Lopez-Ribot, J. L. (2008). A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing. Nat Protoc 3, 1494-1500.
8. Pongraćz, J., Benedek, K., Juhász, E., Iván, M. & Kristóf, K. (2015). In vitro biofilm production of Candida bloodstream isolates: any association with clinical characteristics. J Med Microbiol doi: 10.1099/jmm.0.000207 (In Press).
9. Rajendran, R., Sherry, L., Nile, C. J., Sherriff, A., Johnson, E. M., Hanson, M. F., Williams, C., Munro, C. A., Jones, B. J. & Ramage, G. (2016). Biofilm formation is a risk factor for mortality in patients with Candida albicans bloodstream infection - Scotland, 2012-2013. Clin Microbiol Infect 22, 87-93.
10. Ramage, G., Vande Walle, K., Wickes, B. L. & López-Ribot, J. L. (2001). Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms. Antimicrob Agents Chemother 45, 2475-2479.
11. Taff, H. T., Nett, J. E. & Andes, D. R. (2012). Comparative analysis of Candida biofilm quantitation assays. Med Mycol 50, 214-218.
12. Tellier, R., Krajden, M., Grigoriew, G. A. & Campbell, I. (1992). Innovative endpoint determination system for antifungal susceptibility testing of yeasts. Antimicrob Agents Chemother 36, 1619-1625.
13. Thein, Z. M., Samaranayake, Y. H. & Samaranayake, L. P. (2007). In vitro biofilm formation of Candida albicans and non-albicans Candida species under dynamic and anaerobic conditions. Arch Oral Biol 52, 761-767.
14. Tumbarello, M., Posteraro, B., Trecarichi, E. M., Fiori, B., Rossi, M., Porta, R., de Gaetano Donati, K., La Sorda, M., Spanu, T. & other authors (2007). Biofilm production by Candida species and inadequate antifungal therapy as predictors of mortality for patients with candidemia. J Clin Microbiol 45, 1843-1850.
15. Tumbarello, M., Fiori, B., Trecarichi, E. M., Posteraro, P., Losito, A. R., De Luca, A., Sanguinetti, M., Fadda, G., Cauda, R. & Posteraro, B. (2012). Risk factors and outcomes of candidemia caused by biofilm-forming isolates in a tertiary care hospital. PLoS One 7, e33705