Comparative analysis of Candida biofilm quantitation assays

Medical Mycology

Volumn 50, Issue 2, 2012, Pages 214-218

  Taff, Heather T ^a   Nett, Jeniel E ^a,b   Andes, David R ^a,b,c  

^a Department of Microbiology and Immunology   (United States)

^b UNIVERSITY OF WISCONSIN   (United States)

^c William S Middleton Veterans Hospital   (United States)

Author keywords

Assay; Biofilm; Candida

Indexed keywords

CRYSTAL VIOLET; TETRAZOLIUM;

ARTICLE; BIOFILM; CANDIDA ALBICANS; CELL GROWTH; COLONY FORMATION; CONTROLLED STUDY; DNA DETERMINATION; FUNGUS GROWTH; INTERMETHOD COMPARISON; NONHUMAN; PROTEIN DETERMINATION; QUANTITATIVE ASSAY; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY;

BIOFILMS; CANDIDA ALBICANS; MICROBIAL VIABILITY; MICROBIOLOGICAL TECHNIQUES; MYCOLOGY;

CANDIDA; CANDIDA ALBICANS;

EID: 84855827045     PISSN: 13693786     EISSN: 14602709     Source Type: Journal    
DOI: 10.3109/13693786.2011.580016     Document Type: Article

References (25)

1. Douglas, LJ. Candida biof lms and their role in infection. Trends Microbiol 2003; 11: 30-36. (Pubitemid 36084353)
2. Kojic EM, Darouiche RO. Candida infections of medical devices. Clin Microbiol Rev 2004; 17: 255-267. (Pubitemid 38534029)
3. Kumamoto CA, Vinces MD. Alternative Candida albicans lifestyles: growth on surfaces. Annu Rev Microbiol 2005; 59: 113-133. (Pubitemid 41507426)
4. Mermel LA, Allon M, Bouza E, et al. Clinical practice guidelines for the diagnosis and management of intravascular catheter-related infection: 2009 update by the Infectious Diseases Society of America. Clin Infect Dis 2009; 49: 1-45.
5. Ramage G, Martinez JP, Lopez-Ribot JL. Candida biof lms on implanted biomaterials: a clinically signif cant problem. FEMS Yeast Res 2006; 6: 979-986. (Pubitemid 44568854)
6. Khot PD, Suci PA, Tyler BJ. Candida albicans viability after exposure to amphotericin B: assessment using metabolic assays and colony forming units. J Microbiol Meth 2008; 72: 268-274.
7. Nett J, Lincoln L, Marchillo K, et al. Putative role of beta-1,3 glucans in Candida albicans biof lm resistance. Antimicrob Agents Chemother 2007; 51: 510-520. (Pubitemid 46185268)
8. Nobile CJ, Andes DR, Nett, JE, et al. Critical role of Bcr1-dependent adhesins in C. albicans biof lm formation in vitro and in vivo. PLoS Path 2006; 2: 636-649.
9. Ramage G, Mowat E, Jones B, Williams C, Lopez-Ribot J. Our current understanding of fungal biof lms. Crit Rev Microbiol 2009; 35: 340-355.
10. Ramage G, Vande Walle K, Wickes BL, Lopez-Ribot JL. Standardized method for in vitro antifungal susceptibility testing of Candida albicans biof lms. Antimicrob Agents Chemother 2001; 45: 2475-2479. (Pubitemid 32801898)
11. Chandra J, Mukherjee PK, Ghannoum MA. In vitro growth and analysis of Candida biof lms. Nat Protoc 2008; 3: 1909-1924.
12. Gobor T, Corol G, Ferreira LE, et al. Proposal of protocols using D-glutamine to optimize the 2,3-bis(2-methoxy-4-nitro-5-sulfophenly)-5- [(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) assay for indirect estimation of microbial loads in biof lms of medical importance. J Microbiol Meth 2010; 84: 299-306.
13. Hawser SP, Douglas LJ. Biof lm formation by Candida species on thesurface of catheter materials in vitro. Infect Immun 1994; 62: 915-921. (Pubitemid 24065575)
14. Honraet K, Goetghebeur E, Nelis HJ. Comparison of three assays for the quantif cation of Candida biomass in suspension and CDC reactor grown biof lms. J Microbiol Meth 2005; 63: 287-295. (Pubitemid 41597373)
15. Kuhn DM, Balkis M, Chandra J, Mukherjee PK, Ghannoum MA. Uses and limitations of the XTT assay in studies of Candida growth and metabolism. J Clin Microbiol 2003; 41: 506-508. (Pubitemid 36098635)
16. Nailis H, Coenye T, Van Nieuwerburgh F, Deforce D, Nelis HJ. Development and evaluation of different normalization strategies for gene expression studies in Candida albicans biof lms by real-time PCR. BMC Mol Biol 2006; 7: 1471-2199.
17. Peeters E, Nelis HJ, Coenye T. Comparison of multiple methods for quantif cation of microbial biof lms grown in microtiter plates. J Microbiol Meth 2008; 72: 157-165.
18. Silva S, Henriques M, Martins A, et al. Biof lms of non- Candida albicans Candida species: quantif cation, structure and matrix composition. Med Mycol 2009; 47: 681-689.
19. Davis D, Wilson RB, Mitchell AP. RIM101-dependent and-independent pathways govern pH responses in Candida albicans. Mol Cell Biol 2000; 20: 971-978. (Pubitemid 30044234)
20. Liu Y, Filler SG. Candida albicans Als3: a multifunctional adhesin and invasin. Eukaryot Cell 2011; 10: 168-173.
21. Nobile CJ, Schneider HA, Nett JE, et al. Complementary adhesin function in C. albicans biof lm formation. Curr Biol 2008; 18: 1017-1024.
22. Zhao X, Daniels KJ, Oh SH, et al. Candida albicans Als3p is required for wild-type biof lm formation on silicone elastomer surfaces. Microbiology 2006; 152: 2287-2299. (Pubitemid 44219638)
23. Andes D, Nett J, Oschel P, et al. Development and characterization of an in vivo central venous catheter Candida albicans biof lm model. Infect Immun 2004; 72: 6023-6031. (Pubitemid 39303717)
24. Jin Y, Yip HK, Samaranayake YH, Yau JY, Samaranayake LP. Biof lm-forming ability of Candida albicans is unlikely to contribute to high levels of oral yeast carriage in cases of human immunodef ciency virus infection. J Clin Microbiol 2003; 41: 2961-2967. (Pubitemid 36842285)
25. Uppuluri P, Perumal P, Chaff n WL. Analysis of RNA species of various sizes from stationary-phase planktonic yeast cells of Candida albicans. FEMS Yeast Res 2007; 7: 110-117. (Pubitemid 46122036)