Comparison of bolus injection and constant infusion methods for measuring muscle protein fractional synthesis rate in humans

Metabolism: Clinical and Experimental

Volumn 63, Issue 12, 2014, Pages 1562-1567

  Tuvdendorj, Demidmaa ^a,b   Chinkes, David L ^a,b   Bahadorani, John ^c   Zhang, Xiao Jun ^a,b   Sheffield Moore, Melinda ^a   Killewich, Lois A ^a   Wolfe, Robert R ^d  

^a University of Texas Medical Branch School of Medicine   (United States)

^b SHRINERS HOSPITALS FOR CHILDREN   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES   (United States)

Author keywords

Bolus injection method; Constant infusion method; Fractional synthesis rate; Muscle protein; Stable isotope tracer techniques

Indexed keywords

LEVO [2H5] PHENYLALANINE; MUSCLE PROTEIN; PHENYLALANINE DERIVATIVE; STABLE ISOTOPE; TRACER; UNCLASSIFIED DRUG; DEUTERIUM; PHENYLALANINE; RADIOPHARMACEUTICAL AGENT;

ADULT; AGED; AREA UNDER THE CURVE; ARTICLE; BLOOD SAMPLING; BODY WEIGHT; BOLUS INJECTION; CLINICAL ARTICLE; COMPARATIVE EFFECTIVENESS; CONSTANT INFUSION; CONTINUOUS INFUSION; CONTROLLED STUDY; DRUG BLOOD LEVEL; DRUG INFUSION; FEMALE; FRACTIONAL SYNTHESIS RATE; GASTROCNEMIUS MUSCLE; HUMAN; HUMAN TISSUE; INFUSION RATE; ISOTOPE LABELING; MALE; MASS FRAGMENTOGRAPHY; MIDDLE AGED; MUSCLE BIOPSY; MUSCLE TISSUE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN METABOLISM; PROTEIN SYNTHESIS; BIOSYNTHESIS; COMPARATIVE STUDY; GENETICS; INTRAVENOUS DRUG ADMINISTRATION;

AGED; DEUTERIUM; FEMALE; HUMANS; INFUSIONS, INTRAVENOUS; INJECTIONS, INTRAVENOUS; MALE; MIDDLE AGED; MUSCLE PROTEINS; PHENYLALANINE; RADIOPHARMACEUTICALS;

EID: 84961292217     PISSN: 00260495     EISSN: 15328600     Source Type: Journal    
DOI: 10.1016/j.metabol.2014.09.009     Document Type: Article

References (17)

1. R.R. Wolfe, and D.L. Chinkes Isotopic tracers in metabolic research: principles and practice of kinetic analysis 2005 Wiley-Liss New Jersey
2. P.J. Garlick, and M.A. McNurlan Measurement of protein synthesis in human tissues by the flooding method Curr Opin Clin Nutr Metab Care 1 5 1998 455 460
3. D.L. Chinkes, J. Rosenblatt, and R.R. Wolfe Assessment of the mathematical issues involved in measuring the fractional synthesis rate of protein using the flooding dose technique Clin Sci 84 1993 177 183
4. F. Jahoor, X.J. Zhang, H. Baba, Y. Sakurai, and R.R. Wolfe Comparison of constant infusion and flooding dose techniques to measure muscle protein synthesis rate in dogs J Nutr 122 1992 878 887
5. K. Smith, N. Reynolds, S. Downie, A. Patel, and M.J. Rennie Effects of flooding amino acids on incorporation of labeled amino acids into human muscle protein Am J Physiol 275 1998 E73 E78
6. X.J. Zhang, D.L. Chinkes, and R.R. Wolfe Measurement of muscle protein fractional synthesis and breakdown rates from a pulse tracer injection Am J Physiol 283 2002 E753 E764
7. J.E. Tang, J.J. Manolakos, G.W. Kujbida, P.J. Lysecki, D.R. Moore, and S.M. Phillips Minimal whey protein with carbohydrate stimulates muscle protein synthesis following resistance exercise in trained young men Appl Physiol Nutr Metab 32 2007 1132 1138
8. L.A. Killewich, D. Tuvdendorj, J. Bahadorani, G.C. Hunter, and R.R. Wolfe Amino acids stimulate leg muscle protein synthesis in peripheral arterial disease J Vasc Surg 45 2007 554 560
9. N.N. Abumrad, D. Rabin, M.P. Diamond, and W.W. Lacy Use of a heated superficial hand vein as an alternative site for the measurement of amino acid concentrations and for the study of glucose and alanine kinetics in man Metabolism 30 1981 936 940
10. J. Rosenblatt, D.L. Chinkes, M.H. Wolfe, and R.R. Wolfe Stable isotope tracer analysis by GC-MS, including quantification of isotopomer effects Am J Physiol 263 1992 E584 E596
11. D.L. Chinkes Methods for measuring tissue protein breakdown rate in vivo Curr Opin Clin Nutr Metab Care 8 2005 534 537
12. 13C]leucine Clin Sci (Lond) 77 3 1989 329 336
13. H.C. Dreyer, S. Fujita, J.G. Cadenas, D.L. Chinkes, E. Volpi, and B.B. Rasmussen Resistance exercise increases AMPK activity and reduces 4E-BP1 phosphorylation and protein synthesis in human skeletal muscle J Physiol 576 2006 613 624
14. A.A. Ferrando, K.D. Tipton, M.M. Bamman, and R.R. Wolfe Resistance exercise maintains skeletal muscle protein synthesis during bed rest J Appl Physiol 82 3 1997 807 810
15. B. Mittendorfer, J.L. Andersen, P. Plomgaard, B. Saltin, J.A. Babraj, and K. Smith Protein synthesis rates in human muscles: neither anatomical location nor fibre-type composition are major determinants J Physiol 563 1 2005 203 211
16. D.T. Villareal, G.I. Smith, K. Shah, and B. Mittendorfer Effect of weight loss on the rate of muscle protein synthesis during fasted and fed conditions in obese older adults Obesity 20 9 2012 1780 1786
17. G.I. Smith, B.W. Patterson, and B. Mittendorfer Human muscle protein turnover - why is it so variable? J Appl Physiol 110 2 2011 480 491