Foreskin-isolated keratinocytes provide successful extemporaneous autologous paediatric skin grafts

Journal of Tissue Engineering and Regenerative Medicine

Volumn 10, Issue 3, 2016, Pages 252-260

  Mcheik, Jiad N ^a,b   Barrault, Christine ^c   Pedretti, Nathalie ^c   Garnier, Julien ^c   Juchaux, Franck ^c   Levard, Guillaume ^a   Morel, Franck ^b   Lecron, Jean Claude ^a,b   Bernard, François Xavier ^b,c  

^a POITIERS UNIVERSITY HOSPITAL   (France)

^b UNIVERSITÉ DE POITIERS   (France)

^c BIOalternatives   (France)

Author keywords

Burns; Differentiation; Foreskin; Graft; Keratinocytes; Proliferation

Indexed keywords

MOLECULAR BIOLOGY; TISSUE;

BURN; BURN-IN; DIFFERENTIATION; FORESKIN; GRAFT EFFICIENCY; IN-VITRO; KERATINOCYTES; PROLIFERATION; SKIN GRAFT; VITRO STUDIES;

PEDIATRICS;

ADOLESCENT; ANTIBODY LABELING; ARTICLE; BURN; CELL DIFFERENTIATION; CELL ISOLATION; CELL PROLIFERATION; CELL TRANSPLANTATION; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; DISEASE SEVERITY; EAR; ENGRAFTMENT; EPITHELIZATION; FEMALE; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; INFANT; KERATINOCYTE; MALE; MORBIDITY; PREPUCE; PRESCHOOL CHILD; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SKIN AUTOGRAFT; SKIN TRANSPLANTATION; TISSUE REGENERATION; WOUND HEALING; AUTOTRANSPLANTATION; CELL CULTURE; CELL SEPARATION; CYTOLOGY; EPIDERMIS; GENETICS; IMMUNOHISTOCHEMISTRY; METABOLISM; PATHOLOGY; PROCEDURES; REGENERATION; STEM CELL;

BIOLOGICAL MARKER; MESSENGER RNA;

BIOMARKERS; BURNS; CELL DIFFERENTIATION; CELL SEPARATION; CELLS, CULTURED; CHILD; CHILD, PRESCHOOL; EAR; EPIDERMIS; FORESKIN; HUMANS; IMMUNOHISTOCHEMISTRY; INFANT; KERATINOCYTES; MALE; REGENERATION; RNA, MESSENGER; SKIN TRANSPLANTATION; STEM CELLS; TRANSPLANTATION, AUTOLOGOUS; WOUND HEALING;

EID: 84961198582     PISSN: 19326254     EISSN: 19327005     Source Type: Journal    
DOI: 10.1002/term.1690     Document Type: Article

References (44)

1. Atiyeh BS, Costagliola M. 2007; Cultured epithelial autograft (CEA) in burn treatment: three decades later. Burns 33: 405-413.
2. Baryza MJ, Baryza GA. 1995; The Vancouver Scare Scale: an administration tool and its interrater reliability. J Burn Care Rehabil 16: 535-538.
3. Bernard FX, Pedretti N, Rosdy M, et al. 2002; Comparison of gene expression profiles in human keratinocyte monolayer cultures, reconstituted epidermis and normal human skin: transcriptional effects of retinoid treatments in reconstituted human epidermis. Exp Dermatol 11: 59-74.
4. Boniface K, Bernard FX, Garcia M, et al. 2005; IL-22 inhibits epidermal differentiation and induces proinflammatory gene expression and migration of human keratinocytes. J Immunol 174: 3695-3702.
5. Boyce ST. 2001; Design principles for composition and performance of cultured skin substitutes. Burns 27: 523-533.
6. Boyce ST, Kagan RJ, Meyer NA, et al. 1999; The 1999 clinical research award. Cultured skin substitutes combined with integra artificial skin to replace native skin autograft and allograft for the closure of excised full-thickness burns. J Burn Care Rehabil 20: 453-461.
7. Cervelli V, De Angelis B, Spallone D, et al. 2009; Use of a novel autologous cell-harvesting device to promote epithelialization and enhance appropriate pigmentation in scar reconstruction. Clin Exp Dermatol 35: 776-780.
8. Chester DL, Balderson DS, Papini PG. 2004; A review of keratinocyte delivery to the wound bed. J Burn Care Rehabil 25: 266-275.
9. De Corte P, Verween G, Verbeken G, et al. 2012; Feeder layer- and animal product-free culture of neonatal foreskin keratinocytes: improved performance, usability, quality and safety. Cell Tissue Bank 13: 175-189.
10. Fraulin FO, Bahoric A, Harrop AR, et al. 1998; Autotransplantion of epithelial cells in the pig via an aerosol vehicle. J Burn Care Rehabil 19: 337-345.
11. Fredriksson C, Kratz G, Huss F. 2008; Transplantation of cultured human keratinocytes in single cell suspension: a comparative in vitro study of different application technique. Burns 34: 212-219.
12. Gallico GG, O'Connor NE, Compton CC, et al. 1984; Permanent coverage of large burn wounds with autologous cultured human epithelium. N Engl J Med 311: 448-451.
13. Gerlach JC, Johnen C, McCoy E, et al. 2011; Autologous skin cell spray-transplantation for a deep dermal burn patient in an ambulant treatment room setting. Burns 37: 19-23.
14. Gerlach J, Wolf SE, Johnen C, et al. 2008; Innovative Regenerative Medicine Approaches to Skin Cell-based Therapy for Patients with Burn Injuries. In Principles of Regenerative Medicine No. 76. Elsevier/Academic Press: Burlington, MA; 1298-1321.
15. Gravante G, Di Fede MC, Araco A, et al. 2007; A randomized trial comparing Recell system of epidermal cells delivery versus classic skin grafts for the treatment of deep partial thickness burns. Burns 33: 966-972.
16. Green H 1991; Cultured cells for the treatment of disease. Sci Am 265: 96-102.
17. Green H, Kehinde O, Thomas J. 1979; Growth of cultured human epidermal cells into multiple epithelia suitable for grafting. Proc Natl Acad Sci USA 76: 5665-5668.
18. Guenou H, Nissan X, Larcher F, et al. 2009; Human embryonic stem-cell derivatives for full reconstruction of the pluristratified epidermis: a preclinical study. Lancet 374: 1745-1753.
19. Harris PA, Leigh IM, Navsaria HA. 1998; Pre-confluent keratinocyte grafting: the future for cultured skin replacements? Burns 24: 591-593.
20. Hartmann B, Ekkernkamp A, Johnen C, et al. 2007; Sprayed cultured epithelial autografts for deep dermal burns of the face and neck. Ann Plast Surg 58: 70-73.
21. Hunyadi J, Farkas B, Bertenyi C, et al. 1988; Keratinocyte grafting: a new means of transplantation for full-thickness wounds. J Dermatol Surg Oncol 14: 75-78.
22. Jackson DM. 1991; The evolution of burn treatment in the last 50 years. Burns 17: 329-334.
23. James SE, Booth S, Dheansa B, et al. 2010; Sprayed cultured autologous keratinocytes used alone or in combination with meshed autografts to accelerate wound closure in difficult-to-heal burns patients. Burns 36: 10-20.
24. Jensen UB, Lowell S, Watt FM. 1999; The spatial relationship between stem cells and their progenecy in the basal layer of human epidermis: a new view based on whole-mount labelling and lineage analysis. Development 126: 2409-2418.
25. Jones I, Currie L, Martin R. 2002; A guide to biological skin substitutes. Br J Plast Surg 55: 185-193.
26. Kaiser HW, Stark GB, Kopp J, et al. 1994; Cultured autologous keratinocytes in fibrin glue suspension, exclusively and combined with STS allograft (preliminary clinical and histological report of a new technique). Burns 20: 23-29.
27. Kirsner RS, Marston WA, Snyder RJ, et al. 2012; Spray-applied cell therapy with human allogeneic fibroblasts and keratinocytes for the treatment of chronic venous leg ulcers: a Phase 2, multicentre, double-blind, randomised, placebo-controlled trial. Lancet 12: 60644-60648.
28. Larouche D, Hayward C, Cuffley K, et al. 2005; Keratin 19 as a stem cell marker in vivo and in vitro. Methods Mol Biol 289: 103-110.
29. Larouche D, Tong X, Fradette J, et al. 2008; Vibrissa hair bulge houses two populations of skin epithelial stem cells distinct by their keratin profile. FASEB J 22: 1404.
30. Lavoie A, Claudia Fugere C, Fradette J, et al. 2011; Considerations in the choice of a skin donor site for harvesting keratinocytes containing a high proportion of stem cells for culture in vitro. Burns 37: 440-447.
31. Limat A, Noser FK. 1986; Serial cultivation of single keratinocytes from the outer root sheath of human scalp hair follicles. J Invest Dermatol 87: 485-488.
32. Mcheik JN, Barrault C, Bernard FX, et al. 2010; Quantitative and qualitative study in keratinocytes from foreskin in children. Perspective application in paediatric surgery. Burns 36: 1277-1282.
33. McKeon F 2004; p63 and the epithelial stem cell: more than status quo? Genes Dev 18: 465-469.
34. Michel M, Török N, Godbout MJ, et al. 1996; Keratin 19 as a biochemical marker of skin stem cells in vivo and in vitro: keratin 19 expressing cells are differentially localized in function of anatomic sites, and their number varies with donor age and culture stage. J Cell Sci 109: 1017-1028.
35. Mulekar SV, Ghwish B, Al Issa A, et al. 2008; Treatment of vitiligo lesions by ReCell1 vs conventional melanocyte-keratinocyte transplantation: a pilot study. Br J Dermatol 158: 45-49.
36. Navarro FA, Stoner ML, Park CS, et al. 2000; Sprayed keratinocyte suspensions accelerate epidermal coverage in a porcine microwound model. J Burn Care Rehabil 21: 513-518.
37. Pontiggia L, Biedermann T, Meuli M, et al. 2009; Markers to evaluate the quality and self-renewing potential of engineered human skin substitutes in vitro and after transplantation. J Invest Dermatol 129: 480-490.
38. Rheinwald JG, Green H. 1975; Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 6: 331-343.
39. Schlabe J, Johnen C, Schwartlander R, et al. 2008; Isolation and culture of different epidermal and dermal cell types from human scalp suitable for the development of a therapeutical cell spray. Burns 34: 376-384.
40. Taylor G, Lehrer MS, Jensen PJ, et al. 2000; Involvement of follicular stem cells in forming not only the follicle but also the epidermis. Cell 102: 451-461.
41. Wood FM, Giles N, Stevenson A, et al. 2012; Characterisation of the cell suspension harvested from the dermal epidermal junction using a ReCellW kit. Burns 38: 44-51.
42. Wood FM, Kolybaba ML, Allen P. 2006; The use of cultured epithelial autograft in the treatment of major burn injuries: a critical review of the literature. Burns 32: 395-401.
43. Wood FM, Stoner ML, Fowler BV, et al. 2007; The use of a non-cultured autologous cell suspension and integra1 dermal regeneration template to repair full-thickness skin wounds in a porcine model: a one-step process. Burns 33: 693-700.
44. Zweifel CJ, Contaldo C, Kohler C, et al. 2008; Initial experiences using non-cultured autologous keratinocyte suspension for burn wound closure. J Plast Reconst Aesthet Surg 61: 1-4.