Sprayed cultured autologous keratinocytes used alone or in combination with meshed autografts to accelerate wound closure in difficult-to-heal burns patients

Burns

Volumn 36, Issue 3, 2010, Pages

  James, S Elizabeth ^a   Booth, Simon ^a   Dheansa, Baljit ^a   Mann, Dawn J ^a   Reid, Michael J ^a   Shevchenko, Rostislav V ^a   Gilbert, Philip M ^a  

^a QUEEN VICTORIA HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; AGED; ARTICLE; BURN; CASE REPORT; CELL MIGRATION; DEBRIDEMENT; FEMALE; HISTOPATHOLOGY; HUMAN; HYPERTROPHIC SCAR; KERATINOCYTE; MALE; RISK ASSESSMENT; SEPSIS; SKIN AUTOGRAFT; SKIN BIOPSY; SKIN GRAFT; WOUND CLOSURE; WOUND DRESSING; WOUND HEALING; ADOLESCENT; BURNS; CELL CULTURE; CICATRIX; PROCEDURES; SKIN TRANSPLANTATION; SURGICAL MESH; TRANSPLANTATION; VERY ELDERLY; YOUNG ADULT;

ADOLESCENT; ADULT; AGED, 80 AND OVER; BURNS; CELLS, CULTURED; CICATRIX; FEMALE; HUMANS; KERATINOCYTES; MALE; SKIN TRANSPLANTATION; SURGICAL MESH; WOUND HEALING; YOUNG ADULT;

EID: 77649338774     PISSN: 03054179     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.burns.2008.11.011     Document Type: Article

References (51)

1. Hendon D.N., Barrow R.E., Rutan R.L., Rutan T.C., Desai M.H., and Abston S. A Comparison of conservative versus early excision: therapies in severely burned patients. Ann Surg 209 (1989) 547-553
2. Burke J.F., Bondoc C.C., and Quinby W.C. Primary burn excision and immediate grafting: a method shortening illness. J Trauma 14 5 (1974) 389-395
3. Burke J.F., Quinby Jr. W.C., and Bondoc C.C. Primary excision and prompt grafting as routine therapy for the treatment of thermal burns in children, 1976. Hand Clin 6 2 (1990) 305-317
4. Deitch E.A., Wheelahan T.M., Rose M.P., Clothier J., and Cotter J. Hypertrophic burn scars: analysis of variables. J Trauma 23 (1983) 895-898
5. Tanner J.C., Vandeput J., and Olley J.F. The mesh skin graft. Plast Reconstr Surg 34 3 (1964) 287-292
6. Abdullah A., Blakely P., Hunt R.B.L., Philips L., Herndon D.N., and Robson M.C. Visible scars and self-esteem in paediatric patients with bums. J Burn Care Rehabil 15 (1994) 164-168
7. Alexander J.W., NacMillan B.G., Law E., and Kittur D.S. Treatment of severe burns with widely meshed skin autograft and meshed skin allograft overlay. J Trauma 21 6 (2008) 433-437
8. Kreis R.W., Mackie D.P., Vloemans A.-W.F.P., Hermans R.P., and Hoekstra M.J. Widely expanded postage stamp skin grafts using a modified Meek technique in combination with an allograft overlay. Burns 19 2 (1993) 142-145
9. Peeters R., and Hubens A. The mesh skin graft-true expansion rates. Burns 14 3 (1988) 239-240
10. Kreis R.W., Mackie D.P., Hermans R.P., and Vloemans A.R. Expansion techniques for skin grafts: comparison between mesh and Meek island (sandwich-) grafts. Burns (1994) S39-S42
11. Raff T., Hartmann B., Wagner H., and Germann G. Experience with the modified Meek technique. Acta Chir Plast 142 4 (1996) 142-146
12. Meek C.P. Successful Grafting using the Meek-Wall microdermatome. Am J Surg 96 4 (1958) 557-558
13. Hsieh C., Schuong J.Y., Huang W.S., and Huang T.T. Five years experience of the modified Meek technique in the management of extensive burns. Burns 34 (2008) 350-354
14. Lari A.R., and Gang R.K. Expansion technique for skin grafts (Meek technique) in the treatment of severely burned patients. Burns 27 l (2001) 61-66
15. Zermani R.G.C., Zarabini A., and Trivisonno A. Micrografting in the treatment of severely burned patients. Burns 23 7/8 (1997) 604-607
16. Rheinwald J.G., and Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 6 3 (1975) 331-343
17. O'Connor N.E., Mulliken J.B., Banks-Schlegal S., Kehinde O., and Green H. Grafting of burns with cultured epithelium prepared from autologous epidermal cells. Lancet 1 8211 (1981) 75-78
18. Dascome W., Britt L.D., Jones G.T., Futrell J.W., and Lind J. Cultured keratinocyte grafts: a review and clinical update. Curr Surg 50 (1993) 428-438
19. Chester D.L., Balderson D.S., and Papini R.P. A review of keratinocyte delivery to the wound bed. J Burn Care Rehabil 25 3 (2004) 266-275
20. Woodley D.T., Briggaman R.A., Herzog S.R., Meyers A.A., Peterson H.D., and O'Keefe E.J. Characterization of "neo-dermis" formation beneath cultured human epidermal autografts transplanted on muscle fascia. J Invest Dermatol 95 1 (1990) 20-26
21. Cuono C., Langdon R., and McGuire J. Use of cultured epidermal autografts and dermal allografts as skin replacement after burn injury. Lancet 1 8490 (1986) 1123-1124
22. Cuono C.B., Langdon R., Birchall N., Barttelbort S., and McGuire J. Composite autologous-allogeneic skin replacement: development and clinical application. Plast Reconstr Surg 80 4 (1987) 626-637
23. Braye F., Oddou L., Bertin-Maghit M., Belgacem S., Damour O., Spitalier P., et al. Widely meshed autograft associated with cultured autologous epithelium for the treatment of major burns in children: report of 12 cases. Eur J Pediatr Surg 10 1 (2000) 35-40
24. Leigh I.M., Navsaria H., Purkis P.E., and McKay I. Clinical practice and biological effects of keratinocyte grafting. Ann Acad Med Singapore 20 4 (1991) 549-555
25. Kangesu T., Navsaria H.A., Manek S., Fryer P.R., Leigh I.M., and Green C.J. Kerato-dermal grafts: the importance of dermis for the in vivo growth of cultured keratinocytes. Br J Plast Surg 46 5 (1993) 401-409
26. Phillips T.J., Bhawan J., Leigh I.M., Baum H.J., and Gilchrest B.A. Cultured epidermal autografts and allografts: a study of differentiation and allograft survival. J Am Acad Dermatol 23 2.1 (1990) 189-198
27. Woodley D.T., Peterson H.D., Herzog S.R., Stricklin G.P., Burgeson R.E., Briggaman R.A., et al. Burn wounds resurfaced by cultured epidermal autografts show abnormal reconstitution of anchoring fibrils. JAMA 259 17 (1988) 2566-2571
28. Desai M.H., Mlakar J.M., and McCauley R.L. Lack of long-term durability of cultured keratinocyte burn-wound coverage: a case report. J Burn Care Rehabil 12 6 (1991) 540-545
29. Wood F.M., Kolybaba M.L., and Allen P. The use of cultured epithelial autografts in the treatment of major burn injuries: a critical review of the literature. Burns 32 (2006) 395-401
30. Harris P.A., Leigh I.M., and Navsaria H.A. Pre-confluent keratinocyte grafting: the future for cultured skin replacements?. Burns 24 7 (1998) 591-593
31. Compton C.C., Gill J.M., Bradford D.A., Regauer S., Gallico G.G., and O'Connor N.E. Skin regenerated from cultured epithelial autografts on full-thickness burn wounds from 6 days to 5 years after grafting. A light, electron microscopic and immunohistochemical study. Lab Invest 60 5 (1989) 600-612
32. Carver N., Navsaria H.A., Fryer P., Green C.J., and Leigh I.M. Restoration of basement membrane structure in pigs following keratinocyte autografting. Br J Plast Surg 46 5 (1993) 384-392
33. Putland M., Snelling C.F., Macdonald I., and Iron V.A. Histologic comparison of cultured epithelial autograft and meshed expanded split-thickness skin graft. J Burn Care Rehabil 16 6 (1995) 627-640
34. Adams J.C., and Watt F.M. Changes in keratinocyte adhesion during terminal differentiation: reduction in fibronectin binding precedes alpha 5 beta 1 integrin loss from the cell surface. Cell 63 2 (1990) 425-435
35. Hynes R.O. Integrins: a family of cell surface receptors. Cell 48 4 (1987) 549-554
36. Marchisio P.C., Bondanza S., Cremona O., Cancedda R., and De L.M. Polarized expression of integrin receptors (alpha 6 beta 4, alpha 2 beta 1, alpha 3 beta 1, and alpha v beta 5) and their relationship with the cytoskeleton and basement membrane matrix in cultured human keratinocytes. J Cell Biol 112 4 (1991) 761-773
37. Poumay Y., Roland I.H., Leclercq-Smekens M., and Leloup R. Basal detachment of the epidermis using dispase: tissue spatial organization and fate of integrin alpha 6 beta 4 and hemidesmosomes. J Invest Dermatol 102 1 (1994) 111-117
38. Siwy B.K., and Compton C.C. Cultured epidermis: Indiana University Medical Center's clinical experience. J Burn Care Rehabil 13 (1992) 130-137
39. Lam P.K., Chan E.S., Liew C.T., Yen R.S., Lau H.C., and King W.W. Dermal fibroblasts do not enhance the graft take rate of autologous, cultured keratinocyte suspension on full-thickness wounds in rats. Ann Plast Surg 46 2 (2001) 146-149
40. Leigh I.M., McKay I., Carver N., Navsaria H., and Green C. Skin equivalents and cultured skin: from petri dish to the patient. Wounds 3 (1991) 141-148
41. Hefton J.M., Caldwell D., Biozes D.G., Balin A.K., and Carter D.M. Grafting of skin ulcers with cultured autologous epidermal cells. J Am Acad Dermatol 3 (1986) 399-405
42. Pandya A.N., Woodward B., and Parkhouse N. The use of cultured autologous keratinocytes with integra in the resurfacing of acute burns. Plast Reconstr Surg 102 3 (1998) 825-828
43. MacNeil S. Progress and opportunities for tissue-engineered skin. Nature 445 (2007) 874-880
44. Hartmann B., Ekkernkamp A., Johen C., Gerlach J.C., Belfekruon C., and Kuntscher M.V. Sprayed cultured epithelial autografts for deep dermal burns of the face and neck. Ann Plast Surg 58 l (2007) 70-73
45. Wood F.M., Kolybaba M.L., and Allen P. The use of cultured epithelial autograft in the treatment of major burn wounds: eleven years of clinical experience. Burns 32 (2006) 538-544
46. ®, cultured keratinocytes and split thickness skin graft on wound contraction. Wound Rep Regen 15 (2007) 889-896
47. Navarro F.A., Stoner M.L., Park C.S., Huertas J.C., Lee H.B., Wood F.M., et al. Sprayed keratinocyte suspensions accelerate epidermal coverage in a porcine microwound model. J Burn Care Rehabil 21 (2000) 513-518
48. Atiyeh B.S., and Costagliola M. Cultured epithelial autograft (CEA) in burn treatment: three decades later. Burns 33 (2007) 405-413
49. Moustafa M., Simpson C., Glover M., Dawson R.A., Tesfaye S., Creagh F.M., et al. A new autologous keratinocyte dressing treatment for non-healing diabetic neuropathic foot ulcers. Diabet Med 21 (2004) 786-789
50. Zhu N., Warner R.M., Simpson C., Glover M., Hernon C.A., Kelly J., et al. Treatment of burns and chronic wounds using a new cell transfer dressing for delivery of autologous keratinocytes. Eur J Plast Surg 28 (2005) 319-330
51. Moustafa M., Bullock A.J., Creagh F.M., Heller S., Jeffcoate W., Game F., et al. Randomized, controlled, single-blind study on use of autologous keratinocytes on a transfer dressing to treat nonhealing diabetic ulcers. Regen Med 2 (2007) 887-902