Evaluation in Cameroon of a Novel, Simplified Methodology to Assist Molecular Microbiological Analysis of V. cholerae in Resource-Limited Settings

PLoS Neglected Tropical Diseases

Volumn 10, Issue 1, 2016, Pages

  Debes, Amanda K ^a   Ateudjieu, Jerome ^b,c,d   Guenou, Etiene ^c   Lopez, Anna Lena ^e   Bugayong, Mark Philip ^e,f   Retiban, Pearl Joy ^e,f   Garrine, Marcelino ^g   Mandomando, Inacio ^g,h   Li, Shan ⁱ   Stine, O Colin ⁱ   Sack, David A ^a  

^a JOHNS HOPKINS BLOOMBERG SCHOOL OF PUBLIC HEALTH   (United States)

^b UNIVERSITY OF DSCHANG   (Cameroon)

^c MA SANTE ^*  (Cameroon)

^d MINISTRY OF PUBLIC HEALTH   (Cameroon)

^e UNIVERSITY OF THE PHILIPPINES MANILA   (Philippines)

^f Department of Health   (Philippines)

^g Manhiça Health Research Center   (Mozambique)

^h INSTITUTO NACIONAL DE SAÚDE   (Mozambique)

ⁱ UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CAMEROON; DISEASE SURVEILLANCE; DNA DETERMINATION; DNA EXTRACTION; GENETIC SIMILARITY; GENETIC VARIABILITY; GENOTYPE; MICROBIOLOGICAL EXAMINATION; NONHUMAN; POLYMERASE CHAIN REACTION; PULSED FIELD GEL ELECTROPHORESIS; SEQUENCE HOMOLOGY; VARIABLE NUMBER OF TANDEM REPEAT; VIBRIO CHOLERAE; VIRUS TRANSMISSION; CHOLERA; CLASSIFICATION; CLINICAL TRIAL; GENETIC VARIATION; GENETICS; HUMAN; INFANT; ISOLATION AND PURIFICATION; MICROBIOLOGY; MOLECULAR EPIDEMIOLOGY; MOLECULAR TYPING; MOZAMBIQUE; MULTICENTER STUDY; NEWBORN; PAPER; PHILIPPINES; PRESCHOOL CHILD; PROCEDURES; SPECIMEN HANDLING;

CAMEROON; CHILD, PRESCHOOL; CHOLERA; GENETIC VARIATION; GENOTYPE; HUMANS; INFANT; INFANT, NEWBORN; MINISATELLITE REPEATS; MOLECULAR EPIDEMIOLOGY; MOLECULAR TYPING; MOZAMBIQUE; PAPER; PHILIPPINES; SPECIMEN HANDLING; VIBRIO CHOLERAE;

EID: 84961107804     PISSN: 19352727     EISSN: 19352735     Source Type: Journal    
DOI: 10.1371/journal.pntd.0004307     Document Type: Article

References (25)

1. Chatterjee SN, Chaudhuri K, (2003) Lipopolysaccharides of Vibrio cholerae. I. Physical and chemical characterization. Biochim Biophys Acta 1639: 65–79. 14559113
2. Chen Y, Johnson JA, Pusch GD, Morris JG, Jr.Stine OC, (2007) The genome of non-O1 Vibrio cholerae NRT36S demonstrates the presence of pathogenic mechanisms that are distinct from those of O1 Vibrio cholerae. Infect Immun 75: 2645–2647. 17283087
3. Kendall EA, Chowdhury F, Begum Y, Khan AI, Li S, et al. (2010) Relatedness of Vibrio cholerae O1/O139 isolates from patients and their household contacts, determined by multilocus variable-number tandem-repeat analysis. J Bacteriol 192: 4367–4376. doi: 10.1128/JB.00698-10 20585059
4. Danin-Poleg Y, Cohen LA, Gancz H, Broza YY, Goldshmidt H, et al. (2007) Vibrio cholerae strain typing and phylogeny study based on simple sequence repeats. J Clin Microbiol 45: 736–746. 17182751
5. Rebaudet S, Mengel MA, Koivogui L, Moore S, Mutreja A, et al. (2014) Deciphering the origin of the 2012 cholera epidemic in Guinea by integrating epidemiological and molecular analyses. PLoS Negl Trop Dis 8: e2898. doi: 10.1371/journal.pntd.0002898 24901522
6. Kiiru J, Mutreja A, Mohamed AA, Kimani RW, Mwituria J, et al. (2013) A study on the geophylogeny of clinical and environmental Vibrio cholerae in Kenya. PLoS One 8: e74829. doi: 10.1371/journal.pone.0074829 24066154
7. Azarian T, Ali A, Johnson JA, Mohr D, Prosperi M, et al. (2014) Phylodynamic analysis of clinical and environmental Vibrio cholerae isolates from Haiti reveals diversification driven by positive selection. MBio 5.
8. Ghosh R, Nair GB, Tang L, Morris JG, Sharma NC, et al. (2008) Epidemiological study of Vibrio cholerae using variable number of tandem repeats. FEMS Microbiol Lett 288: 196–201. doi: 10.1111/j.1574-6968.2008.01352.x 18811655
9. Alam M, Hasan NA, Sadique A, Bhuiyan NA, Ahmed KU, et al. (2006) Seasonal cholera caused by Vibrio cholerae serogroups O1 and O139 in the coastal aquatic environment of Bangladesh. Appl Environ Microbiol 72: 4096–4104. 16751520
10. Stine OC, Alam M, Tang L, Nair GB, Siddique AK, et al. (2008) Seasonal cholera from multiple small outbreaks, rural Bangladesh. Emerg Infect Dis 14: 831–833. doi: 10.3201/eid1405.071116 18439375
11. Bhuiyan NA, Nusrin S, Ansaruzzaman M, Islam A, Sultana M, et al. (2012) Genetic characterization of Vibrio cholerae O1 strains isolated in Zambia during 1996–2004 possessing the unique VSP-II region of El Tor variant. Epidemiol Infect 140: 510–518. doi: 10.1017/S0950268811000926 21676349
12. Mohamed AA, Oundo J, Kariuki SM, Boga HI, Sharif SK, et al. (2012) Molecular epidemiology of geographically dispersed Vibrio cholerae, Kenya, January 2009-May 2010. Emerg Infect Dis 18: 925–931. doi: 10.3201/eid1806.111774 22607971
13. Lam C, Octavia S, Reeves PR, Lan R, (2012) Multi-locus variable number tandem repeat analysis of 7th pandemic Vibrio cholerae. BMC Microbiol 12: 82. doi: 10.1186/1471-2180-12-82 22624829
14. Kotloff KL, Nataro JP, Blackwelder WC, Nasrin D, Farag TH, et al. (2013) Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study. Lancet 382: 209–222. doi: 10.1016/S0140-6736(13)60844-2 23680352
15. Nhampossa T, Mandomando I, Acacio S, Quinto L, Vubil D, et al. (2015) Diarrheal Disease in Rural Mozambique: Burden, Risk Factors and Etiology of Diarrheal Disease among Children Aged 0–59 Months Seeking Care at Health Facilities. PLoS One 10: e0119824. doi: 10.1371/journal.pone.0119824 25973880
16. Debes AK, Ateudjieu J., Guenou E., Ebile W., Sonkoua I.T., Njimbia A.C., Steinwald P., Ram M., Sack D.A., (In Press) Clinical and Environmental Surveillance for Vibrio cholerae in Resource Contstrained Areas: Application during a one year surveillance in the Far North Region of Cameroon. Am J Trop Med Hyg.
17. Hijmans RJ, Guarino, L., Bussink, C., Mathur, P., Cruz, M., Barrentes, I (2015) DIVA-GIS 7.5.
18. ArcGIS (2015). 10.0 ed. Redlands, California: Esri.
19. Debes AC, S; Sack, D.A. (2015) Manual for Detecting Vibrio cholerae O1 from Fecal Samples using an Enriched Dipstick Assay—A Low-Cost, Simplified Method of Confirming Cholera. Baltimore, MD.
20. Panchalingam S, Antonio M, Hossain A, Mandomando I, Ochieng B, et al. (2012) Diagnostic microbiologic methods in the GEMS-1 case/control study. Clin Infect Dis 55 Suppl 4: S294–302. doi: 10.1093/cid/cis754 23169941
21. Kain KC, Lanar DE, (1991) Determination of genetic variation within Plasmodium falciparum by using enzymatically amplified DNA from filter paper disks impregnated with whole blood. J Clin Microbiol 29: 1171–1174. 1864936
22. Nandi B, Nandy RK, Mukhopadhyay S, Nair GB, Shimada T, et al. (2000) Rapid method for species-specific identification of Vibrio cholerae using primers targeted to the gene of outer membrane protein OmpW. J Clin Microbiol 38: 4145–4151. 11060082
23. Hoshino K, Yamasaki S, Mukhopadhyay AK, Chakraborty S, Basu A, et al. (1998) Development and evaluation of a multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O1 and O139. FEMS Immunol Med Microbiol 20: 201–207. 9566491
24. Bauer A, Rorvik LM, (2007) A novel multiplex PCR for the identification of Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus. Lett Appl Microbiol 45: 371–375. 17897378
25. Rashed SM, Azman AS, Alam M, Li S, Sack DA, et al. (2014) Genetic variation of Vibrio cholerae during outbreaks, Bangladesh, 2010–2011. Emerg Infect Dis 20: 54–60. doi: 10.3201/eid2001.130796 24377372