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The egg contains approximately 300, 000 copies of the 16, 569 base-pair mitochondrial genome compared to one copy of the 3, 031, 042, 417 base-pair nuclear genome. I am indebted to Neville Cobbe for pointing this out to me.
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For while MST starts with three gametes, combines elements of the two eggs, and generates one ‘three parent’ embryo by fertilisation, PNT starts with four gametes, generates two embryos by IVF, and then deconstructs these embryos. The PNT embryo is not itself generated by fertilisation, directly from gametes, but by reconstruction from two embryos, in a process very similar to somatic cell nuclear transfer (that is, cloning). PNT thus strains our understanding of biological parenthood perhaps to breaking point, notwithstanding that the embryo produced is very like an embryo generated by the ‘three parent’ MST technique
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