Drug testing and flow cytometry analysis on a large number of uniform sized tumor spheroids using a microfluidic device

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Patra, Bishnubrata ^a,b   Peng, Chien Chung ^a   Liao, Wei Hao ^a   Lee, Chau Hwang ^a,b,c   Tung, Yi Chung ^a  

^a RESEARCH CENTER FOR APPLIED SCIENCES   (Taiwan)

^b NATIONAL YANG MING UNIVERSITY   (Taiwan)

^c NATIONAL TAIWAN UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

CELL CULTURE TECHNIQUE; DRUG EFFECTS; DRUG SCREENING; FLOW CYTOMETRY; HUMAN; MICROFLUIDIC ANALYSIS; MULTICELLULAR SPHEROID; PROCEDURES;

CELL CULTURE TECHNIQUES; DRUG SCREENING ASSAYS, ANTITUMOR; FLOW CYTOMETRY; HUMANS; MICROFLUIDIC ANALYTICAL TECHNIQUES; SPHEROIDS, CELLULAR;

EID: 84959563012     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep21061     Document Type: Article

References (38)

1. Stewart, B. W., Wild, C. P. World Cancer Report 2014, International Agency fro Research on Cancer, World Health Organization (2014).
2. Boyd, M. R., Paull, K. D. Some practical considerations and applications of the national cancer institute in vitro anticancer drug discovery screen. Drug Develop. Res. 34, 91-109 (1995).
3. Zhang, X. et al. Development of an in vitro multicellular tumor spheroid model using microencapsulation and its application in anticancer drug screening and testing. Biotechnol. Progr. 21, 1289-1296 (2005).
4. Tung, Y.-C. et al. High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array. Analyst 136, 473-478 (2011).
5. Pampaloni, F., Reynaud, E. G., Stelzer, E. H. The third dimension bridges the gap between cell culture and live tissue. Nat. Rev. Mol. Cell Bio. 8, 839-845 (2007).
6. Sutherland, R. M. Cell and environment interactions in tumor microregions: the multicell spheroid model. Science 240, 177-184 (1988).
7. Kunz-Schughart, L. A., Freyer, J. P., Hofstaedter, F., Ebner, R. The use of 3-D cultures for high-throughput screening: the multicellular spheroid model. J. Biomol. Screen. 9, 273-285 (2004).
8. Friedrich, F., Ebner, R., Kunz-Schughart, L. A. Experimental anti-tumor therapy in 3-D: spheroids-old hat or new challenge? Int. J. Radiat. Biol. 83, 849-871 (2007).
9. Lin, R.-Z., Chang, H.-Y. Recent advances in three-dimensional multicellular spheroid culture for biomedical research. Biotechnol. J. 3, 1172-1184 (2008).
10. Desoize, B., Jardillier, J. Multicellular resistance: a paradigm for clinical resistance? Crit. Rev. Oncol. Hematol. 36, 193-207 (2000).
11. Yu, L., Chen, M. C., Cheung, K. C. Droplet-based microfluidic system for multicellular tumor spheroid formation and anticancer drug testing. Lab Chip 10, 2424-2432 (2010).
12. Ziolkowska, K. et al. Long-term three-dimensional cell culture and anticancer drug activity evaluation in a microfluidic chip. Biosens. Bioelectron. 40, 68-74 (2013).
13. Das, T. et al. Empirical chemosensitivity testing in a spheroid model of ovarian cancer using a microfluidics-based multiplex platform. Biomicrofluidics 7, 11805 (2013).
14. Kwapiszewska, K., Michalczuk, A., Rybka, M., Kwapiszewski, R., Brzozka, Z. A microfluidic-based platform for tumour spheroid culture, monitoring and drug screening. Lab Chip 14, 2096-2104 (2014).
15. Chen, Y.-C., Lou, X., Zhang, Z., Ingram, P., Yoon, E. High-throughput cancer cell sphere formation for characterizing the efficacy of photo dynamic therapy in 3D cell cultures, Sci. Rep. 5, 12175 (2015).
16. Givan, A. L. Flow Cytometry-First Principles, Second Ed. Wiley, 2001.
17. Tung, Y.-C., Zhang, M., Lin, C.-T., Kurabayashi, K., Skerlos, S. J. PDMS-based opto-fluidic micro flow cytometer with two-color, multi-angle fluorescence detection capability using PIN photodiodes. Sensor. Actuat. B-Chem. 98, 356-367 (2004).
18. Patra, B. et al. A microfluidic device for uniform-sized cell spheroids formation, culture, harvesting and flow cytometry analysis. Biomicrofluidics 7, 54114 (2013).
19. Patra, B. et al. Migration and vascular lumen formation of endothelial cells in cancer cell spheroids of various sizes. Biomicrofluidics 8, 052109 (2014).
20. Lu, J.-C., Liao, W.-H., Tung, Y.-C. Magnet-assisted device-level alignment for fabrication of membrane-sandwiched polydimethylisiloxane (PDMS) microfluidic devices. J. Microme. Microeng. 22, 75006-75013 (2012).
21. Barry, M. A., Behnke, C. A., Eastman, A. Activation of programmed cell death (apoptosis) by cisplatin, other anticancer drugs, toxins and hyperthermia. Biochem. Pharmacol. 40, 2353-2362 (1990).
22. Siddik, Z. H. Cisplatin: mode of cytotoxic action and molecular basis of resistance. Oncogene 22 7265-7279 (2003).
23. Ou, X., Chen, Y., Cheng, X., Zhang, X., He, Q. Potentiation of resveratrol-induced apoptosis by matrine in human hepatoma HepG2 cells. Oncol. Rep. 32, 2803-2809 (2014).
24. Carter, L. G., D'Orazio, J. A., Pearson, K. J. Resveratrol and cancer: focus on in vivo evidence. Endocr. Relat. Cancer 21, R209-225 (2014).
25. Jounaidi, Y., Waxman, D. J. Combination of the bioreductive drug tirapazamine with the chemotherapeutic prodrug cyclophosphamide for P450/P450-reductase-based cancer gene therapy. Cancer Res. 60, 3761-3769 (2000).
26. Peters, K. B., Wang, H., Brown, J. M., Iliakis, G. Inhibition of DNA replication by tirapazamine. Cancer Res. 61, 5425-5431 (2001).
27. Reddy, S. B., Williamson, S. K. Tirapazamine: a novel agent targeting hypoxic tumor cells. Expert. Opin. Investig. Drugs 18, 77-87 (2009).
28. Zembruski, N. C., Stache, V., Haefeli, W. E., Weiss, J. 7-Aminoactinomycin D for apoptosis staining in flow cytometry. Anal. Biochem. 429, 79-81 (2012).
29. van Engeland, M., Nieland, N. J., Ramaekers, F. C., Schutte, B., Reutelingsperger, C. P. Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure. Cytometry 31, 1-9 (1998).
30. Anada, T., Fukuda, J., Sai, Y., Suzuki, O. An oxygen-permeable spheroid culture system for the prevention of central hypoxia and necrosis of spheroids. Biomaterials 33, 8430-8441 (2012).
31. Di Domenico, F., Foppoli, C., Coccia, R., Perluigi, M. Antioxidants in cervical cancer: chemopreventive and chemotherapeutic effects of polyphenols. BBA-Mol. Basis Dis. 1822, 737-747 (2012).
32. Notas, G. et al. Resveratrol exerts its antiproliferative effect on HepG2 hepatocellular carcinoma cells, by inducing cell cycle arrest, and NOS activation. Biochim. Biophys. 1760, 1657-1666 (2006).
33. Thomas, D. D., Liu, X., Kantrow, S. P., Lancaster, Jr., J. R. The biological lifetime of nitric oxide: Implications for the perivascular dynamics of NO and O2. Proc. Natl. Acad. Sci. USA 98, 355-360 (2001).
34. Mitrovic, B. et al. Nitric oxide induces necrotic but not apoptotic cell death in oligodendrocytes. Neuroscience 65, 531-539 (1995).
35. Uchiyama, T. et al. Nitric oxide induces caspase-dependent apoptosis and necrosis in neonatal rat cardiomyocytes. J. Mol. Cell Cardiol. 34, 1049-1061 (2002).
36. Grimes, D. R., Kelly, C., Bloch, K., Partridge, M. A method for estimating the oxygen consumption rate in multicellular tumour spheroids. J. R. Soc. Interface. 11, 20131124 (2014).
37. Venook, A. P. Treatment of hepatocellular carcinoma: too many options ? J. Clin. Oncol. 12, 1323-1334 (1994).
38. American Cancer Society, Liver Cancer. (Available online at http://www.cancer.org/acs/groups/cid/documents/webcontent/003114-pdf.pdf) (2015) Date of access: 30/12/2015.