Isolation of live Borrelia burgdorferi sensu lato spirochaetes from patients with undefined disorders and symptoms not typical for Lyme borreliosis

Clinical Microbiology and Infection

Volumn 22, Issue 3, 2016, Pages 267.e9-267.e15

  Rudenko, N ^a,b   Golovchenko, M ^a,b   Vancova, M ^a   Clark, K ^c   Grubhoffer, L ^a,d   Oliver, J H ^b  

^a INSTITUTE OF PARASITOLOGY   (Czech Republic)

^b GEORGIA SOUTHERN UNIVERSITY   (United States)

^c UNIVERSITY OF NORTH FLORIDA   (United States)

^d UNIVERSITY OF SOUTH BOHEMIA   (Czech Republic)

Author keywords

Antibiotic treatment; Live Borrelia bissettii; Live Borrelia burgdorferi; Lyme borreliosis; Recovery of live spirochaetes

Indexed keywords

DOXYCYCLINE; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M;

ADULT; ANTIBIOTIC THERAPY; ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATION; BORRELIA BISSETTII; BORRELIA BURGDORFERI; BORRELIA BURGDORFERI SENSU STRICTO; CLINICAL ARTICLE; CULTURE MEDIUM; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMAN; LYME DISEASE; MALE; MIDDLE AGED; MULTILOCUS SEQUENCE TYPING; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; SPIROCHETE; WESTERN BLOTTING; BACTERIAL GENE; BACTERIUM IDENTIFICATION; CLASSIFICATION; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; ULTRASTRUCTURE;

BACTERIAL TYPING TECHNIQUES; BORRELIA BURGDORFERI GROUP; FEMALE; GENES, BACTERIAL; HUMANS; LYME DISEASE; MALE; MIDDLE AGED;

EID: 84959495382     PISSN: 1198743X     EISSN: 14690691     Source Type: Journal    
DOI: 10.1016/j.cmi.2015.11.009     Document Type: Article

References (35)

1. Rudenko N., Golovchenko M., Grubhoffer L., Oliver J.H. Updates on Borrelia burgdorferi sensu lato complex with respect to public health. Ticks Tick Borne Dis 2011, 2:123-128.
2. Steere A.C., Malawista S.E., Snydman D.R., Shope R.E., Andiman W.A., Ross M.R., et al. Lyme arthritis: an epidemic of oligoarticular arthritis in children and adults in three Connecticut communities. Arthritis Rheum 1997, 20:7-17.
3. Steer A.C. Lyme disease. N Engl J Med 1989, 321:586-596.
4. Pal U., Fikrig E. Adaptation of Borrelia burgdorferi in the vector and vertebrate host. Microbes Infect 2003, 5:659-666.
5. Kurtenbach K., De Michelis S., Etti S., Schäfer S.M., Sewell H.S., Brade V., et al. Host association of Borrelia burgdorferi sensu lato-the key role of host complement. Trends Microbiol 2002, 10:74-79.
6. Hodzic E., Feng S., Freet K.J., Barthold S.W. Borrelia burgdorferi population dynamics and prototype gene expression during infection of immunocompetent and immunodeficient mice. Infect Immun 2003, 71:5042-5055.
7. Ramamoorthi N., Narasimhan S., Pal U., Bao F., Yang X.F., Fish D., et al. The Lyme disease agent exploits a tick protein to infect the mammalian host. Nature 2005, 436:573-577.
8. Brorson O., Brorson S.H. In vitro conversion of Borrelia burgdorferi to cystic forms in spinal fluid, and transformation to mobile spirochetes by incubation in BSK-medium. Infection 1998, 26:144-150.
9. Miklossy J., Kasas S., Zurn A.D., McCall S., Yu S., McGeer P.L. Persisting atypical and cystic forms of Borrelia burgdorferi and local inflammation in Lyme borreliosis. J Neuroinflammation 2008, 5:40.
10. Hodzic E., Imai D., Feng S., Barthold S.W. Resurgence of persisting non-cultivable Borrelia burgdorferi following antibiotic treatment in mice. PLoS ONE 2014, 9:e86907.
11. Bockenstedt L.K., Mao J., Hodzic E., Barthold S.W., Fish D. Detection of attenuated, noninfectious spirochetes in Borrelia burgdorferi-infected mice after antibiotic treatment. J Infect Dis 2002, 186:1430-1437.
12. Preac-Mursic V., Wilske B., Schierz G. European Borrelia burgdorferi isolated from humans and ticks: culture conditions and antibiotic susceptibility. Zbl Bakteriol Hyg A 1986, 236:112-118.
13. Ružić-Sabljić E., Strle F. Comparison of growth of Borrelia afzelii, B. garinii, and B. burgdorferi sensu stricto in MKP and BSK-II medium. Int J Med Microbiol 2004, 294:407-412.
14. Maraspin V., Ruzić-Sabljić E., Cimperman J., Lotric-Furlan S., Jurca T., Picken R.N., et al. Isolation of Borrelia burgdorferi sensu lato from blood of patients with erythema migrans. Infection 2001, 29:65-70.
15. Rudenko N., Golovchenko M., Grubhoffer L., Oliver J.H. Borrelia carolinensis sp. nov., a new (14th) member of the Borrelia burgdorferi sensu lato complex from the southeastern region of the United States. J Clin Microbiol 2009, 47:134-141.
16. Rudenko N., Golovchenko M., Honig V., Mallátová N., Krbková L., Mikulásek P., et al. Detection of Borrelia burgdorferi sensu stricto ospC alleles associated with human Lyme borreliosis worldwide in non-human-biting tick Ixodes affinis and rodent hosts in Southeastern United States. Appl Environ Microbiol 2013, 79:1444-1453.
17. Rudenko N., Golovchenko M., Belfiore M.N., Grubhoffer L., Oliver J.H. Divergence of Borrelia burgdorferi sensu lato spirochetes could be driven by the host: diversity of Borrelia strains isolated from ticks feeding on a single bird. Parasit Vectors 2014, 7:4. 10.1186/1756-3305-7-4.
18. Hoen A.G., Margos G., Bent S.J., Diuk-Wasser M.A., Barbour A., Kurtenbach K., et al. Phylogeography of Borrelia burgdorferi in the eastern United States reflects multiple independent Lyme disease emergence events. Proc Natl Acad Sci USA 2009, 106:15013-15018.
19. Qiu W.G., Bruno J.F., McCaig W.D., Xu Y., Livey I., Schriefer M.E., et al. Wide distribution of a high-virulence Borrelia burgdorferi clone in Europe and North America. Emerg Infect Dis 2008, 14:1097-1104.
20. Hanincová K., Liveris D., Sandigursky S., Wormser G.P., Schwartz I. Borrelia burgdorferi sensu stricto is clonal in patients with early Lyme borreliosis. Appl Environ Microbiol 2008, 74:5008-5014.
21. Thyresson N. The penicillin treatment of acrodermatitis atrophicans chronica (Herxheimer). Acta Derm Venerol (Stockh) 1949, 29:572-621.
22. Hollström E. Succesful treatment of erythema migrans Afzelius. Acta Derm Venereol 1951, 31:235-243.
23. Embers M., Barthold S.W. Borrelia burgdorferi persistence post-antibiotic treatment. The pathogenic spirochetes: strategies for evasion of host immunity and persistence 2012, 229-252. Springer, New York. M.E. Embers (Ed.).
24. Hodzic E., Feng S., Holden K., Freet K.J., Barthold S.W. Persistence of Borrelia burgdorferi following antibiotic treatment in mice. Antimicrob Agents Chemother 2008, 52:1728-1736.
25. Barthold S.W., Hodzic E., Imai D.M., Feng S., Yang X., Luft B.J. Ineffectiveness of tigecycline against persistent Borrelia burgdorferi. Antimicrob Agents Chemother 2010, 54:643-651.
26. Embers M.E., Barthold S.W., Borda J.T., Bowers L., Doyle L., Hodzic E., et al. Persistence of Borrelia burgdorferi in rhesus macaques following antibiotic treatment of disseminated infection. PLoS One 2012, 7:e29914.
27. Marques A., Telford S.R., Turk S.-P., Chung E., Williams C., Dardicket K., et al. Xenodiagnosis to detect Borrelia burgdorferi infection: a first-in-human study. Clin Infect Dis 2014, 58:937-945.
28. Bockenstedt L.K., Radolf J.D. Xenodiagnosis for posttreatment Lyme disease syndrome: resolving the conundrum or adding to it?. Clin Infect Dis 2014, 58:946-948.
29. Sapi E., Kaur N., Anyanwu S., Luecke D.F., Datar A., Patel S., et al. Evaluation of in-vitro antibiotic susceptibility of different morphological forms of Borrelia burgdorferi. Infect Drug Resist 2011, 4:97-113.
30. Margos G., Gatewood A.G., Aanensen D.M., Hanincová K., Terekhova D., Vollmer S.A., et al. MLST of housekeeping genes captures geographic population structure and suggests a European origin of Borrelia burgdorferi. Proc Natl Acad Sci USA 2008, 105:8730-8735.
31. Rudenko N., Golovchenko M., Mokrácek A., Piskunová N., Růžek D., Mallatová N., et al. Detection of Borrelia bissettii in cardiac valve tissue of a patient with endocarditis and aortic valve stenosis in the Czech Republic. J Clin Microbiol 2008, 46:3540-3543.
32. Rudenko N., Golovchenko M., Růzek D., Piskunova N., Mallátová N., Grubhoffer L. Molecular detection of Borrelia bissettii DNA in serum samples from patients in the Czech Republic with suspected borreliosis. FEMS Microbiol Lett 2009, 292:274-281.
33. Girard Y.A., Fedorova N., Lane R.S. Genetic diversity of Borrelia burgdorferi and detection of B. bissettii-like DNA in serum of north-coastal California residents. J Clin Microbiol 2010, 49:945-954.
34. Golovchenko M., Vancová M., Clark K., Oliver J.H., Grubhoffer L., Rudenko N. A divergent strain isolated from a resident of the southeastern United States was identified by MLST analysis as Borrelia bissettii. Parasites Vectors 2015.
35. Oliver J.H. Lyme borreliosis in the southern United States: a review. J Parasitol 1996, 82:926-935.