Single-molecule microscopy of molecules tagged with GFP or RFP derivatives in mammalian cells using nanobody binders

Methods

Volumn 88, Issue , 2015, Pages 89-97

  Platonova, Evgenia ^a,b   Winterflood, Christian M ^a,b   Junemann, Alexander ^c   Albrecht, David ^a,b   Faix, Jan ^c   Ewers, Helge ^a,b,d  

^a KING'S COLLEGE LONDON   (United Kingdom)

^b ETH ZURICH   (Switzerland)

^c HANNOVER MEDICAL SCHOOL   (Germany)

^d FREIE UNIVERSITÄT BERLIN   (Germany)

Author keywords

Nanobody; PALM; Single domain antibody; Single particle tracking (SPT); Single molecule localization microscopy (SMLM); Spectral demixing direct stochastic reconstruction microscopy (SD dSTORM); STORM; VHH

Indexed keywords

GREEN FLUORESCENT PROTEIN; NANOBODY; RED FLUORESCENT PROTEIN; FLUORESCENT DYE; PHOTOPROTEIN;

ACCURACY; ARTICLE; BINDING AFFINITY; BINDING SITE; CELL STRUCTURE; IMAGE ANALYSIS; MEASUREMENT; MICROSCOPY; MOLECULAR IMAGING; NONHUMAN; PARTICLE SIZE; PHYSICAL PHENOMENA; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SINGLE MOLECULE LOCALIZATION MICROSCOPY; SINGLE PARTICLE TRACKING; STRUCTURE ANALYSIS; ANIMAL; CELL CULTURE; FLUORESCENCE MICROSCOPY; FLUORESCENT ANTIBODY TECHNIQUE; HUMAN; IMAGE PROCESSING; IMMUNOLOGY; PROCEDURES; RAT; STAINING;

ANIMALS; CELLS, CULTURED; FLUORESCENT ANTIBODY TECHNIQUE; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MOLECULAR IMAGING; RATS; SINGLE-DOMAIN ANTIBODIES; STAINING AND LABELING;

EID: 84959488694     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2015.06.018     Document Type: Article

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