Transformation of probiotic yeast and their recovery from gastrointestinal immune tissues following oral gavage in mice

Journal of Visualized Experiments

Volumn 2016, Issue 108, 2016, Pages

  Hudson, Lauren E ^a   Stewart, Taryn P ^a   Fasken, Milo B ^a   Corbett, Anita H ^a   Lamb, Tracey J ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Auxotroph; Gavage; GFP; Heterologous protein; Immunology; Issue 108; Peyer s patch; Probiotic; Saccharomyces boulardii; URA3; UV mutagenesis

Indexed keywords

PROBIOTIC AGENT;

ANIMAL; DRUG DELIVERY SYSTEM; IMMUNOLOGY; INTESTINE; ISOLATION AND PURIFICATION; MICROBIOLOGY; MOUSE; MUCOSAL IMMUNITY; ORAL DRUG ADMINISTRATION; SACCHAROMYCES CEREVISIAE;

ADMINISTRATION, ORAL; ANIMALS; DRUG DELIVERY SYSTEMS; IMMUNITY, MUCOSAL; INTESTINES; MICE; PROBIOTICS; SACCHAROMYCES CEREVISIAE;

EID: 84959431779     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/53453     Document Type: Article

References (41)

1. Edwards-Ingram, L., Gitsham, P., et al. Genotypic and physiological characterization of Saccharomyces boulardii, the probiotic strain of Saccharomyces cerevisiae. Applied and environmental microbiology. 73 (8), 2458-67 (2007).
2. Hudson, L. E., Fasken, M. B., et al. Functional Heterologous Protein Expression by Genetically Engineered Probiotic Yeast Saccharomyces boulardii. PloS one. 9 (11), 1-12 (2014).
3. Guthrie, C., & Fink, G. R. Methods in Enzymology, Volume 149: Guide to Yeast Genetics and Molecular Biology. 3-933 (1991).
4. DiCarlo, J. E., Norville, J. E., Mali, P., Rios, X., Aach, J., & Church, G. M. Genome engineering in Saccharomyces cerevisiae using CRISPRCas systems. Nucleic acids research. 41 (7), 4336-43 (2013).
5. Sander, J. D., & Joung, J. K. CRISPR-Cas systems for editing, regulating and targeting genomes. Nature biotechnology. 32 (4), 347-55 (2014).
6. Lorenz, M. C., Muir, R. S., Lim, E., McElver, J., Weber, S. C., & Heitman, J. Gene disruption with PCR products in Saccharomyces cerevisiae. Gene. 158, 113-117 (1995).
7. Hamedi, H., Misaghi, A., Modarressi, M. H., Salehi, T. Z., Khorasanizadeh, D., & Khalaj, V. Generation of a Uracil Auxotroph Strain of the Probiotic Yeast Saccharomyces boulardii as a Host for the Recombinant Protein Production. Avicenna Journal of Medical Biotechnology. 5 (1), 29-34 (2013).
8. Hinnen, A., Hicks, J. B., & Fink, G. R. Transformation of yeast. Proceedings of the National Academy of Sciences of the United States of America. 75 (4), 1929-33 (1978).
9. Hashimoto, H., Morikawa, H., Yamada, K., & Kimura, A. A novel method for transformation of intact yeast cells by electroinjection of plasmid DNA. Appl Microbiol Biotechnol. 21, 336-9 (1985).
10. Becker, D., & Guarente, L. High-efficiency transformation of yeast by electroporation. Methods Enzymol. 194, 182-7 (1991).
11. Benatuil, L., Perez, J. M., Belk, J., & Hsieh, C.-M. An improved yeast transformation method for the generation of very large human antibody libraries. Protein engineering, design & selection: PEDS. 23 (4), 155-9 (2010).
12. Ito, H., Fukuda, Y., & Murata, K. Transformation of intact yeast cells treated with alkali cations. Journal of Bacteriology. 153 (1), 166-168 (1983).
13. Kawai, S., Hashimoto, W., & Murata, K. Transformation of Saccharomyces cerevisiae and other fungi: methods and possible underlying mechanism. Bioengineered bugs. 1 (6), 395-403 (2010).
14. Zheng, H. Z., Liu, H. H., et al. Yeast transformation process studied by fluorescence labeling technique. Bioconjugate Chemistry. 16, 250-254 (2005).
15. Gietz, R. D., & Woods, R. A. Genetic transformation of yeast. BioTechniques. 30 (4), 816-831 (2001).
16. JoVE Science Education Database. The ELISA Method. Basic Methods in Cellular and Molecular Biology. (2015).
17. Gallagher, S., & Chakavarti, D. Immunoblot analysis. Journal of visualized experiments: JoVE. (16) (2008).
18. Horn, C. C., Kimball, B. A., et al. Why Can’t Rodents Vomit? A Comparative Behavioral, Anatomical, and Physiological Study. PLoS ONE. 8(4) (2013).
19. Hoggatt, A. F., Hoggatt, J., Honerlaw, M., & Pelus, L. M. A spoonful of sugar helps the medicine go down: a novel technique to improve oral gavage in mice. Journal of the American Association for Laboratory Animal Science: JAALAS. 49 (3), 329-334 (2010).
20. Johnson, M., Shayne, C. G., & Kemper, C. J. The rat. Animal Models in Toxicology. 50-193 (2006).
21. Brown, A. P., Dinger, N., & Levine, B. S. Stress produced by gavage administration in the rat. Contemporary topics in laboratory animal science/American Association for Laboratory Animal Science. 39, 17-21 (2000).
22. Jacoby, R., Fox, J., & Davisson, M. Biology and diseases of mice. Laboratory animal medicine., 35-133 (2002).
23. Schulz, O., & Pabst, O. Antigen sampling in the small intestine. Trends in immunology. 34 (4), 155-61 (2013).
24. McDole, J. R., Wheeler, L. W., et al. Goblet cells deliver luminal antigen to CD103+ dendritic cells in the small intestine. Nature. 483 (7389), 345-9 (2012).
25. Farache, J., Koren, I., et al. Luminal bacteria recruit CD103+ dendritic cells into the intestinal epithelium to sample bacterial antigens for presentation. Immunity. 38 (3), 581-95 (2013).
26. Burke, D., Dawson, D., & Stearns, T. Methods in yeast genetics: a Cold Spring Harbor Laboratory course manual. Cold Spring Harbor Lab Press (2000).
27. Boeke, J. D., LaCroute, F., & Fink, G. R. A positive selection for mutants lacking orotidine-5’-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Molecular & general genetics: MGG. 197 (2), 345-6 (1984).
28. Chattoo, B. B., Sherman, F., Azubalis, D. A., Fjellstedt, T. A., Mehnert, D., & Ogur, M. Selection of lys2 mutants of the yeast Saccharomyces cerevisiae by the utilization of alpha-aminoadipate. Genetics. 93, 51-65 (1979).
29. Toyn, J. H., Gunyuzlu, P. L., White, W. H., Thompson, L. a & Hollis, G. F. A counterselection for the tryptophan pathway in yeast: 5-fluoroanthranilic acid resistance. Yeast (Chichester, England). 16 (6), 553-60 (2000).
30. Singh, A., & Sherman, F. Genetic and physiological characterization of met15 mutants of Saccharomyces cerevisiae: a selective system for forward and reverse mutations. Genetics. 81, 75-97 (1975).
31. Singh, A., & Sherman, F. Characteristics and relationships of mercury resistant mutants and methionine auxotrophs of yeast. Journal of Bacteriology. 118 (3), 911-918 (1974).
32. McCoy, S. L., Hausman, F. A., et al. Quantification of DNA binding to cell-surfaces by flow cytometry. Journal of Immunological Methods. 241,141-146 (2000).
33. Gietz, R. D., Schiestl, R. H., Willems, A. R., & Woods, R. A. Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure. Yeast. 11, 355-360 (1995).
34. Zhang, Z., & Chisti, Y. Plasmid stability in recombinant saccharomyces cerevisiae. Science. 14 (4), 401-435 (1996).
35. Lorang, J. M., Tuori, R. P., Martinez, J. P., Sawyer, T. L., Redman, R. S., & Rollins, J. a Papers in Plant Pathology Green Fluorescent Protein Is Lighting Up Fungal Biology Green Fluorescent Protein Is Lighting Up Fungal Biology. Society. 67 (5), 1987-1994 (2001).
36. Thompson, J. R., Register, E., Curotto, J., Kurtz, M., & Kelly, R. An improved protocol for the preparation of yeast cells for transformation by electroporation. Yeast. 14, 565-571 (1998).
37. Damsch, S., Eichenbaum, G., et al. Gavage-related reflux in rats: identification, pathogenesis, and toxicological implications (review). Toxicologic pathology. 39, 348-360 (2011).
38. Desai, M., Labhasetwar, V., Amidon, G., & Levy, R. Gastrointestinal uptake of biodegradable nanoparticles: effect of particle size. Pharma. 13(12) (1994).
39. Shakweh, M., Ponchel, G., & Fattal, E. Particle uptake by Peyer’s patches: a pathway for drug and vaccine delivery. Expert opinion on drug delivery. 1 (1) (2004).
40. Szymanski, E. P., & Kerscher, O. Budding yeast protein extraction and purification for the study of function, interactions, and post-translational modifications. Journal of visualized experiments: JoVE. (80), e50921 (2013).
41. Hashimoto, S., Ogura, M., Aritomi, K., Hoshida, H., Nishizawa, Y., & Akada, R. Isolation of Auxotrophic Mutants of Diploid Industrial Yeast Strains after UV Mutagenesis. Applied and environmental microbiology. 71 (1), 312-319 (2005).