Cell contractility arising from topography and shear flow determines human mesenchymal stem cell fate

Scientific Reports

Volumn 6, Issue , 2016, Pages

  Sonam, Surabhi ^a,b   Sathe, Sharvari R ^b   Yim, Evelyn K F ^a,b   Sheetz, Michael P ^a,c   Lim, Chwee Teck ^a,b  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^c NONE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

6-(4-(3-(METHYLSULFONYL)BENZYLAMINO)-5-(TRIFLUOROMETHYL)PYRIMIDIN-2-YLAMINO)-3,4-DIHYDROQUINOLIN-2(1H)-ONE; MYOSIN ADENOSINE TRIPHOSPHATASE; QUINOLONE DERIVATIVE; SULFONE;

BIOPHYSICS; BONE DEVELOPMENT; CELL ADHESION; CELL CULTURE; CELL DIFFERENTIATION; CYTOLOGY; DRUG EFFECTS; EXTRACELLULAR MATRIX; FOCAL ADHESION; HUMAN; MECHANICAL STRESS; MESENCHYMAL STROMA CELL; METABOLISM; PHYSIOLOGY;

ACTOMYOSIN; BIOPHYSICAL PHENOMENA; CELL ADHESION; CELL DIFFERENTIATION; CELLS, CULTURED; EXTRACELLULAR MATRIX; FOCAL ADHESIONS; HUMANS; MESENCHYMAL STROMAL CELLS; OSTEOGENESIS; QUINOLONES; STRESS, MECHANICAL; SULFONES;

EID: 84958280523     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep20415     Document Type: Article

References (33)

1. Engler, A. J., Sen, S., Sweeney, H. L., Discher, D. E. Matrix Elasticity Directs Stem Cell Lineage Specification. Cell 126, 677-689 (2006).
2. McBeath, R., Pirone, D. M., Nelson, C. M., Bhadriraju, K., Chen, C. S. Cell Shape, Cytoskeletal Tension, and RhoA Regulate Stem Cell Lineage Commitment. Dev. Cell 6, 483-495 (2004).
3. Gao, L., McBeath, R., Chen, C. S. Stem cell shape regulates a chondrogenic versus myogenic fate through Rac1 and N-cadherin. Stem Cells 28, 564-72 (2010).
4. Yim, E. K. F., Pang, S. W., Leong, K. W. Synthetic nanostructures inducing differentiation of human mesenchymal stem cells into neuronal lineage. Exp. Cell Res. 313, 1820-9 (2007).
5. Teo, B. K. K. et al. Nanotopography modulates mechanotransduction of stem cells and induces differentiation through focal adhesion kinase. ACS Nano 7, 4785-98 (2013).
6. Arnsdorf, E. J., Tummala, P., Kwon, R. Y., Jacobs, C. R. Mechanically induced osteogenic differentiation-the role of RhoA, ROCKII and cytoskeletal dynamics. J. Cell Sci. 122, 546-553 (2009).
7. Kilian, K. a, Bugarija, B., Lahn, B. T., Mrksich, M. Geometric cues for directing the differentiation of mesenchymal stem cells. Proc. Natl. Acad. Sci. U. S. A. 107, 4872-7 (2010).
8. Yao, X., Peng, R., Ding, J. Effects of aspect ratios of stem cells on lineage commitments with and without induction media. Biomaterials 34, 930-939 (2013).
9. Dalby, M. J. et al. The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder. Nat. Mater. 6, 997-1003 (2007).
10. Oh, S. et al. Stem cell fate dictated solely by altered nanotube dimension. Proc. Natl. Acad. Sci. U. S. A. 106, 2130-5 (2009).
11. Ji, L., LaPointe, V. L. S., Evans, N. D., Stevens, M. M. Changes in embryonic stem cell colony morphology and early differentiation markers driven by colloidal crystal topographical cues. Eur. Cell. Mater. 23, 135-46 (2012).
12. Klein, G. The extracellular matrix of the hematopoietic microenvironment. Experientia 51, 914-926 (1995).
13. Drzeniek, Z. et al. Proteoglycan synthesis in haematopoietic cells: isolation and characterization of heparan sulphate proteoglycans expressed by the bone-marrow stromal cell line MS-5. Biochem. J. 327 (Pt 2, 473-480 (1997).
14. Gallagher, J. O., McGhee, K. F., Wilkinson, C. D. W., Riehle, M. O. Interaction of animal cells with ordered nanotopography. IEEE Trans. Nanobioscience 1, 24-8 (2002).
15. Anselme, K. et al. The interaction of cells and bacteria with surfaces structured at the nanometre scale. Acta Biomater. 6, 3824-46 (2010).
16. Das, R. K., Zouani, O. F., Labrugere, C., Oda, R., Durrieu, M.-C. Influence of nanohelical shape and periodicity on stem cell fate. ACS Nano 7, 3351-61 (2013).
17. del Rio, A. et al. Stretching single talin rod molecules activates vinculin binding. Science 323, 638-641 (2009).
18. Humphries, J. D. et al. Vinculin controls focal adhesion formation by direct interactions with talin and actin. J. Cell Biol. 179, 1043-1057 (2007).
19. Ziegler, W. H., Gingras, A. R., Critchley, D. R., Emsley, J. Integrin connections to the cytoskeleton through talin and vinculin. Biochem. Soc. Trans. 36, 235-239 (2008).
20. Dumbauld, D. W. et al. Contractility Modulates Cell Adhesion Strengthening Through Focal Adhesion Kinase and Assembly of Vinculin-Containing Focal Adhesions. J. Cell. Physiol. 223, 746-756 (2010).
21. Margadant, F. et al. Mechanotransduction in vivo by repeated talin stretch-relaxation events depends upon vinculin. PLoS Biol. 9, (2011).
22. Hirata, H., Tatsumi, H., Lim, C. T., Sokabe, M. Force-dependent vinculin binding to talin in live cells: a crucial step in anchoring the actin cytoskeleton to focal adhesions. Am. J. Physiol. Cell Physiol. 306, C607-20 (2014).
23. McMurray, R. J. et al. Nanoscale surfaces for the long-term maintenance of mesenchymal stem cell phenotype and multipotency. Nat. Mater. 10, 637-44 (2011).
24. Chen, W. et al. Nanotopography Influences Adhesion, Spreading, and Self-Renewal of Human Embryonic Stem Cells. ACS Nano 6, 4094-4103 (2012).
25. Weinbaum, S., Cowin, S. C., Zeng, Y. A model for the excitation of osteocytes by mechanical loading-induced bone fluid shear stresses. J. Biomech. 27, 339-60 (1994).
26. Titushkin, I., Cho, M. Modulation of cellular mechanics during osteogenic differentiation of human mesenchymal stem cells. Biophys. J. 93, 3693-702 (2007).
27. Yang, Y., Kulangara, K., Sia, J., Wang, L., Leong, K. W. Engineering of a microfluidic cell culture platform embedded with nanoscale features. Lab Chip 11, 1638-1646 (2011).
28. Wong, S. T., Teo, S. K., Park, S., Chiam, K. H., Yim, E. K. F. Anisotropic rigidity sensing on grating topography directs human mesenchymal stem cell elongation. Biomech. Model. Mechanobiol. 13, 27-39 (2014).
29. Caplan, A. I. Mesenchymal stem cells. J. Orthop. Res. 9, 641-50 (1991).
30. Crisan, M. et al. A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell 3, 301-13 (2008).
31. Friedenstein, A. J., Chailakhyan, R. K., Latsinik, N. V, Panasyuk, A. F., Keiliss-Borok, I. V. Stromal cells responsible for transferring the microenvironment of the hematopoietic tissues: Cloning In Vitro and Retransplantation In Vivo. Transplantation 17, (1974).
32. Riveline, D. et al. Focal contacts as mechanosensors: Externally applied local mechanical force induces growth of focal contacts by an mDia1-dependent and ROCK-independent mechanism. J. Cell Biol. 153, 1175-1185 (2001).
33. Goessler, U. R. et al. Integrin expression in stem cells from bone marrow and adipose tissue during chondrogenic differentiation. Int. J. Mol. Med. 21, 271-9 (2008).