Chamber specific gene expression landscape of the zebrafish heart

PLoS ONE

Volumn 11, Issue 1, 2016, Pages

  Singh, Angom Ramcharan ^a   Sivadas, Ambily ^a,b   Sabharwal, Ankit ^a,b   Vellarikal, Shamsudheen Karuthedath ^a,b   Jayarajan, Rijith ^a   Verma, Ankit ^a   Kapoor, Shruti ^a,b   Joshi, Adita ^a   Scaria, Vinod ^a,b   Sivasubbu, Sridhar ^a,b  

^a CSIR INSTITUTE OF GENOMICS AND INTEGRATIVE BIOLOGY   (India)

^b ACADEMY OF SCIENTIFIC AND INNOVATIVE RESEARCH ACSIR   (India)

Author keywords

[No Author keywords available]

Indexed keywords

TRANSCRIPTOME; ZEBRAFISH PROTEIN;

ANIMAL TISSUE; ARTICLE; BULBUS ARTERIOSUS; CONTROLLED STUDY; GENE EXPRESSION; HEART; HEART ATRIUM; HEART DEVELOPMENT; HEART DISEASE; HEART VENTRICLE; NONHUMAN; PATHOGENESIS; RNA SEQUENCE; ZEBRA FISH; ANIMAL; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; IN SITU HYBRIDIZATION; METABOLISM; SEQUENCE ANALYSIS;

ANIMALS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, DEVELOPMENTAL; HEART ATRIA; HEART VENTRICLES; HUMANS; IN SITU HYBRIDIZATION; SEQUENCE ANALYSIS, RNA; TRANSCRIPTOME; ZEBRAFISH; ZEBRAFISH PROTEINS;

EID: 84958225776     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0147823     Document Type: Article

References (92)

1. Bakkers J. Zebrafish as a model to study cardiac development and human cardiac disease. Cardiovasc Res. 2011; 91: 279-288. doi: 10.1093/cvr/cvr098 PMID: 21602174
2. Nemtsas P, Wettwer E, Christ T, Weidinger G, Ravens U. Adult zebrafish heart as a model for human heart? An electrophysiological study. J Mol Cell Cardiol. 2010; 48: 161-171. doi: 10.1016/j.yjmcc. 2009.08.034 PMID: 19747484
3. Laflamme MA, Murry CE. Heart regeneration. Nature. 2011; 19; 473: 326-335. doi: 10.1038/nature10147 PMID: 21593865
4. Liu Y, Morley M, Brandimarto J, Hannenhalli S, Hu Y, Ashley EA, et al. RNA-Seq identifies novel myocardial gene expression signatures of heart failure. Genomics. 2015; 105: 83-89. doi: 10.1016/j.ygeno. 2014.12.002 PMID: 25528681
5. Gong W, Koyano-Nakagawa N, Li T, Garry DJ. Inferring dynamic gene regulatory networks in cardiac differentiation through the integration of multi-dimensional data. BMC Bioinformatics. 2015; 16:74. doi: 10.1186/s12859-015-0460-0: 74-0460. PMID: 25887857
6. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001; 29: E45. PMID: 11328886
7. Iruretagoyena JI, Davis W, Bird C, Olsen J, Radue R, Teo BA, et al. Metabolic gene profile in early human fetal heart development. Mol Hum Reprod. 2014; 20: 690-700. doi: 10.1093/molehr/gau026 PMID: 24674993
8. Rodius S, Nazarov PV, Nepomuceno-Chamorro IA, Jeanty C, Gonzalez-Rosa JM, Ibberson M, et al. Transcriptional response to cardiac injury in the zebrafish: Systematic identification of genes with highly concordant activity across in vivo models. BMC Genomics. 2014; 15:852. doi: 10.1186/1471-2164-15-852: 852-15. PMID: 25280539
9. Lescroart F, Chabab S, Lin X, Rulands S, Paulissen C, Rodolosse A, et al. Early lineage restriction in temporally distinct populations of Mesp1 progenitors during mammalian heart development. Nat Cell Biol. 2014; 16: 829-840. doi: 10.1038/ncb3024 PMID: 25150979
10. Zhou J, Gao J, Liu Y, Gu S, Zhang X, An X, et al. Human atrium transcript analysis of permanent atrial fibrillation. Int Heart J. 2014; 55: 71-77. PMID: 24463922
11. Chen J, Wang HY, Zeng CY. Transcriptome network analysis of potential candidate genes for heart failure. Genet Mol Res. 2013; 12: 4687-4697. doi: 10.4238/2013.October.18.7 PMID: 24222245
12. Dang MQ, Zhao XC, Lai S, Wang X, Wang L, Zhang YL, et al. Gene expression profile in the early stage of angiotensin II-induced cardiac remodeling: A time series microarray study in a mouse model. Cell Physiol Biochem. 2015; 35: 467-476. doi: 10.1159/000369712 PMID: 25613478
13. Geng J, Picker J, Zheng Z, Zhang X, Wang J, Hisama F, et al. Chromosome microarray testing for patients with congenital heart defects reveals novel disease causing loci and high diagnostic yield. BMC Genomics. 2014; 15:1127. doi: 10.1186/1471-2164-15-1127: 1127-15. PMID: 25516202
14. Liu Y, Li G, Lu H, Li W, Li X, Liu H, et al. Expression profiling and ontology analysis of long noncoding RNAs in post-ischemic heart and their implied roles in ischemia/reperfusion injury. Gene. 2014; 543: 15-21. doi: 10.1016/j.gene.2014.04.016 PMID: 24726549
15. Liu H, Chen GX, Liang MY, Qin H, Rong J, Yao JP, et al. Atrial fibrillation alters the microRNA expression profiles of the left atria of patients with mitral stenosis. BMC Cardiovasc Disord. 2014; 14:10. doi: 10.1186/1471-2261-14-10: 10-14. PMID: 24461008
16. Li D, Chen G, Yang J, Fan X, Gong Y, Xu G, et al. Transcriptome analysis reveals distinct patterns of long noncoding RNAs in heart and plasma of mice with heart failure. PLoS One. 2013; 8: E77938. doi: 10.1371/journal.pone.0077938 PMID: 24205036
17. Song G, Shen Y, Zhu J, Liu H, Liu M, Shen YQ, et al. Integrated analysis of dysregulated lncRNA expression in fetal cardiac tissues with ventricular septal defect. PLoS One. 2013; 8: E77492. doi: 10. 1371/journal.pone.0077492 PMID: 24147006
18. Muthusamy S, DeMartino AM, Watson LJ, Brittian KR, Zafir A, Dassanayaka S, et al. MicroRNA-539 is up-regulated in failing heart, and suppresses O-GlcNAcase expression. J Biol Chem. 2014; 289: 29665-29676. doi: 10.1074/jbc.M114.578682 PMID: 25183011
19. Franco D, Lamers WH, Moorman AF. Patterns of expression in the developing myocardium: Towards a morphologically integrated transcriptional model. Cardiovasc Res. 1998; 38: 25-53. PMID: 9683906
20. Shendure J. The beginning of the end for microarrays? Nat Methods. 2008; 5: 585-587. doi: 10.1038/nmeth0708-585 PMID: 18587314
21. Malone JH, Oliver B. Microarrays, deep sequencing and the true measure of the transcriptome. BMC Biol. 2011; 9:34. doi: 10.1186/1741-7007-9-34: 34-39. PMID: 21627854
22. Najmabadi H, Hu H, Garshasbi M, Zemojtel T, Abedini SS, Chen W, et al. Deep sequencing reveals 50 novel genes for recessive cognitive disorders. Nature. 2011; 478: 57-63. doi: 10.1038/nature10423 PMID: 21937992
23. Pan Q, Shai O, Lee LJ, Frey BJ, Blencowe BJ. Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing. Nat Genet. 2008; 40: 1413-1415. doi: 10.1038/ng.259 PMID: 18978789
24. Sultan M, Schulz MH, Richard H, Magen A, Klingenhoff A, Scherf M, et al. A global view of gene activity and alternative splicing by deep sequencing of the human transcriptome. Science. 2008; 321: 956-960. doi: 10.1126/science.1160342 PMID: 18599741
25. Yang KC, Yamada KA, Patel AY, Topkara VK, George I, Cheema FH, et al. Deep RNA sequencing reveals dynamic regulation of myocardial noncoding RNAs in failing human heart and remodeling with mechanical circulatory support. Circulation. 2014; 129: 1009-1021. doi: 10.1161/CIRCULATIONAHA. 113.003863 PMID: 24429688
26. Leptidis S, El AH, Lok SI, de WR, Olieslagers S, Kisters N, A deep sequencing approach to uncover the miRNOME in the human heart. PLoS One. 2013; 8: E57800. doi: 10.1371/journal.pone.0057800 PMID: 23460909
27. Liu HL, Zhu JG, Liu YQ, Fan ZG, Zhu C, Qian LM. Identification of the microRNA expression profile in the regenerative neonatal mouse heart by deep sequencing. Cell Biochem Biophys. 2014; 70: 635-642. doi: 10.1007/s12013-014-9967-7 PMID: 24756729
28. Bao ZZ, Bruneau BG, Seidman JG, Seidman CE, Cepko CL. Regulation of chamber-specific gene expression in the developing heart by Irx4. Science. 1999; 19; 283: 1161-1164. PMID: 10024241
29. Yamagishi H, Yamagishi C, Nakagawa O, Harvey RP, Olson EN, Srivastava D. The combinatorial activities of Nkx2.5 and dHAND are essential for cardiac ventricle formation. Dev Biol. 2001; 239: 190-203. PMID: 11784028
30. Bruneau BG, Bao ZZ, Tanaka M, Schott JJ, Izumo S, Cepko CL. Cardiac expression of the ventriclespecific homeobox gene Irx4 is modulated by Nkx2-5 and dHand. Dev Biol. 2000; 217: 266-277. PMID: 10625552
31. Zhao XS, Gallardo TD, Lin L, Schageman JJ, Shohet RV. Transcriptional mapping and genomic analysis of the cardiac atria and ventricles. Physiol Genomics. 2002; 12: 53-60. PMID: 12502795
32. Sucov HM. Molecular insights into cardiac development. Annu Rev Physiol. 1998; 60:287-308.: 287-308. PMID: 9558465
33. Kaynak B, von HA, Mebus S, Seelow D, Hennig S, Vogel J, et al. Genome-wide array analysis of normal and malformed human hearts. Circulation. 2003; 20; 107: 2467-2474. PMID: 12742993
34. Patowary A, Purkanti R, Singh M, Chauhan R, Singh AR, Swarnkar M, et al. A sequence-based variation map of zebrafish. Zebrafish. 2013; 10: 15-20. doi: 10.1089/zeb.2012.0848 PMID: 23590399
35. Westerfield M. The zebrafish book. A guide for the laboratory use of zebrafish (Danio rerio). Eugene. 2000. University of Oregon Press.
36. Kaushik K, Leonard VE, Kv S, Lalwani MK, Jalali S, Patowary A, et al. Dynamic expression of long noncoding RNAs (lncRNAs) in adult zebrafish. PLoS One. 2013; 8: E83616. doi: 10.1371/journal.pone. 0083616 PMID: 24391796
37. Bolger AM, Lohse M, Usadel B. Trimmomatic: A flexible trimmer for Illumina sequence data. Bioinformatics. 2014; 30: 2114-2120. doi: 10.1093/bioinformatics/btu170 PMID: 24695404
38. Dobin A, Davis CA, Schlesinger F, Drenkow J, Zaleski C, Jha S, Batut P, et al. STAR: Ultrafast universal RNA-seq aligner. Bioinformatics. 2013; 29: 15-21. doi: 10.1093/bioinformatics/bts635 PMID: 23104886
39. Trapnell C, Williams BA, Pertea G, Mortazavi A, Kwan G, Van Baren MJ, et al. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nat Biotechnol. 2010; 28: 511-515. doi: 10.1038/nbt.1621 PMID: 20436464
40. Rice P, Longden I, Bleasby A. EMBOSS: The European Molecular Biology Open Software Suite. Trends Genet. 2000; 16: 276-277. PMID: 10827456
41. Kong L, Zhang Y, Ye ZQ, Liu XQ, Zhao SQ, Wei L. CPC: Assess the protein-coding potential of transcripts using sequence features and support vector machine. Nucleic Acids Res. 2007; 35: W345-W349. PMID: 17631615
42. Lin MF, Jungreis I, Kellis M. PhyloCSF: A comparative genomics method to distinguish protein coding and non-coding regions. Bioinformatics. 2011; 27: I275-i282. doi: 10.1093/bioinformatics/btr209 PMID: 21685081
43. Eddy SR. Accelerated Profile HMM Searches. PLoS Comput Biol. 2011; 7: E1002195. doi: 10.1371/journal.pcbi.1002195 PMID: 22039361
44. Punta M, Coggill PC, Eberhardt RY, Mistry J, Tate J, Boursnell C. The Pfam protein families database. Nucleic Acids Res. 2012; 40: D290-D301. doi: 10.1093/nar/gkr1065 PMID: 22127870
45. Pauli A, Valen E, Lin MF, Garber M, Vastenhouw NL, Levin JZ, et al. Systematic identification of long noncoding RNAs expressed during zebrafish embryogenesis. Genome Res. 2012; 22: 577-591. doi: 10.1101/gr.133009.111 PMID: 22110045
46. Ulitsky I, Shkumatava A, Jan CH, Sive H, Bartel DP. Conserved function of lincRNAs in vertebrate embryonic development despite rapid sequence evolution. Cell.2011; 147: 1537-1550. doi: 10.1016/j. cell.2011.11.055 PMID: 22196729
47. Wang J, Duncan D, Shi Z, Zhang B. WEB-based GEne SeT AnaLysis Toolkit (WebGestalt): Update 2013. Nucleic Acids Res. 2013; 41: W77-W83. doi: 10.1093/nar/gkt439 PMID: 23703215
48. Chew GL, Pauli A, Rinn JL, Regev A, Schier AF, Valen E. Ribosome profiling reveals resemblance between long non-coding RNAs and 5' leaders of coding RNAs. Development. 2013; 140: 2828-2834. doi: 10.1242/dev.098343 PMID: 23698349
49. Ingolia NT, Lareau LF, Weissman JS. Ribosome profiling of mouse embryonic stem cells reveals the complexity and dynamics of mammalian proteomes. Cell. 2011; 147: 789-802. doi: 10.1016/j.cell. 2011.10.002 PMID: 22056041
50. Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, et al. The SILVA ribosomal RNA gene database project: Improved data processing and web-based tools. Nucleic Acids Res. 2013; 41: D590-D596. doi: 10.1093/nar/gks1219 PMID: 23193283
51. Langmead B, Salzberg SL. Fast gapped-read alignment with Bowtie 2. Nat Methods. 2012; 9: 357-359. doi: 10.1038/nmeth.1923 PMID: 22388286
52. Kim D, Pertea G, Trapnell C, Pimentel H, Kelley R, Salzberg SL. TopHat2: Accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions. Genome Biol. 2013; 14: R36-14. doi: 10.1186/gb-2013-14-4-r36 PMID: 23618408
53. Cox MP, Peterson DA, Biggs PJ. SolexaQA: At-a-glance quality assessment of Illumina second-generation sequencing data. BMC Bioinformatics. 2010; 11:485. doi: 10.1186/1471-2105-11-485: 485-11. PMID: 20875133
54. Quinlan AR. BEDTools: The Swiss-Army Tool for Genome Feature Analysis. Curr Protoc Bioinformatics. 2014; 47:11.12.1-11.12.34. doi: 10.1002/0471250953.bi1112s47: 11.
55. Ruzicka L, Bradford YM, Frazer K, Howe DG, Paddock H, Ramachandran S, et al. ZFIN, The zebrafish model organism database: Updates and new directions. Genesis. 2015;20 doi:10.1002/dvg.22868: 10.
56. Hamosh A, Scott AF, Amberger JS, Bocchini CA, McKusick VA. Online Mendelian Inheritance in Man (OMIM), a knowledgebase of human genes and genetic disorders. Nucleic Acids Res. 2005; 33: D514-D517. PMID: 15608251
57. Berdougo E, Coleman H, Lee DH, Stainier DY, Yelon D. Mutation of weak atrium/atrial myosin heavy chain disrupts atrial function and influences ventricular morphogenesis in zebrafish. Development. 2003; 130: 6121-6129. PMID: 14573521
58. Somi S, Houweling AC, Buffing AA, Moorman AF, Van Den Hoff MJ. Expression of cVg1 mRNA during chicken embryonic development. Anat Rec A Discov Mol Cell Evol Biol. 2003; 273: 603-608. PMID: 12808645
59. Thisse C, Thisse B. High-resolution in situ hybridization to whole-mount zebrafish embryos. Nat Protoc. 2008; 3: 59-69. doi: 10.1038/nprot.2007.514 PMID: 18193022
60. Sogah VM, Serluca FC, Fishman MC, Yelon DL, Macrae CA, Mably JD. Distinct troponin C isoform requirements in cardiac and skeletal muscle. Dev Dyn. 2010; 239: 3115-3123. doi: 10.1002/dvdy. 22445 PMID: 20925115
61. Hoffmann B, Schmidt-Traub H, Perrot A, Osterziel KJ, Gessner R. First mutation in cardiac troponin C, L29Q, in a patient with hypertrophic cardiomyopathy. Hum Mutat. 2001; 17: 524.
62. Mogensen J, Murphy RT, Kubo T, Bahl A, Moon JC, Klausen IC, et al. Frequency and clinical expression of cardiac troponin I mutations in 748 consecutive families with hypertrophic cardiomyopathy. J Am Coll Cardiol. 2004; 44: 2315-2325. PMID: 15607392
63. Wooldridge AA, Fortner CN, Lontay B, Akimoto T, Neppl RL, Facemire C. Deletion of the protein kinase A/protein kinase G target SMTNL1 promotes an exercise-adapted phenotype in vascular smooth muscle. J Biol Chem. 2008; 283: 11850-11859. doi: 10.1074/jbc.M708628200 PMID: 18310078
64. Sertie AL, Sossi V, Camargo AA, Zatz M, Brahe C, Passos-Bueno MR. Collagen XVIII, containing an endogenous inhibitor of angiogenesis and tumor growth, plays a critical role in the maintenance of retinal structure and in neural tube closure (Knobloch syndrome). Hum Mol Genet. 2000; 9: 2051-2058. PMID: 10942434
65. Everett AW, Umeda PK, Sinha AM, Rabinowitz M, Zak R. Expression of myosin heavy chains during thyroid hormone-induced cardiac growth. Fed Proc. 1986; 45: 2568-2572. PMID: 3758376
66. Posch MG, Waldmuller S, Muller M, Scheffold T, Fournier D, Andrade-Navarro MA, et al. Cardiac alpha-myosin (MYH6) is the predominant sarcomeric disease gene for familial atrial septal defects. PLoS One. 2011; 6: E28872. doi: 10.1371/journal.pone.0028872 PMID: 22194935
67. Holm H, Gudbjartsson DF, Sulem P, Masson G, Helgadottir HT, Zanon C, et al. A rare variant in MYH6 is associated with high risk of sick sinus syndrome. Nat Genet. 2011; 43: 316-320. doi: 10.1038/ng. 781 PMID: 21378987
68. Laing NG, Dye DE, Wallgren-Pettersson C, Richard G, Monnier N, Lillis S, et al. Mutations and polymorphisms of the skeletal muscle alpha-actin gene (ACTA1). Hum Mutat. 2009; 30: 1267-1277. doi: 10. 1002/humu.21059 PMID: 19562689
69. Klein SC, Haas RC, Perryman MB, Billadello JJ, Strauss AW. Regulatory element analysis and structural characterization of the human sarcomeric mitochondrial creatine kinase gene. J Biol Chem. 1991; 266: 18058-18065. PMID: 1917943
70. Pipkin W, Johnson JA, Creazzo TL, Burch J, Komalavilas P, Brophy C. Localization, macromolecular associations, and function of the small heat shock-related protein HSP20 in rat heart. Circulation. 2003; 107: 469-476. PMID: 12551873
71. Tessier DJ, Komalavilas P, Panitch A, Joshi L, Brophy CM. The small heat shock protein (HSP) 20 is dynamically associated with the actin cross-linking protein actinin. J Surg Res. 2003; 111: 152-157. PMID: 12842460
72. Zhang R, Xu X. Transient and transgenic analysis of the zebrafish ventricular myosin heavy chain (vmhc) promoter: An inhibitory mechanism of ventricle-specific gene expression. Dev Dyn. 2009; 238: 1564-1573. doi: 10.1002/dvdy.21929 PMID: 19322764
73. Seidman JG, Seidman C. The genetic basis for cardiomyopathy: From mutation identification to mechanistic paradigms. Cell. 2001; 104: 557-567. PMID: 11239412
74. Thisse B, Heyer V, Lux A, Alunni V, Degrave A, Seiliez I, et al. Spatial and temporal expression of the zebrafish genome by large-scale in situ hybridization screening. Methods Cell Biol. 2004; 77:505-19.: 505-519. PMID: 15602929
75. Raymond CS, Shamu CE, Shen MM, Seifert KJ, Hirsch B, Hodgkin J, et al. Evidence for evolutionary conservation of sex-determining genes. Nature. 1998; 391: 691-695. PMID: 9490411
76. Ying M, Chen B, Tian Y, Hou Y, Li Q, Shang X, et al. Nuclear import of human sexual regulator DMRT1 is mediated by importin-beta. Biochim Biophys Acta. 2007; 1773: 804-813. PMID: 17459496
77. Zhuchenko O,WehnertM, Bailey J, Sun ZS, Lee CC. Isolation, mapping, and genomic structure of an X-linked gene for a subunit of human mitochondrial complex I. Genomics. 1996; 37: 281-288. PMID: 8938439
78. Pattaro C, Kottgen A, Teumer A, Garnaas M, Boger CA, Fuchsberger C, et al. Genome-wide association and functional follow-up reveals new loci for kidney function. PLoS Genet. 1996; 2012; 8: E1002584. doi: 10.1371/journal.pgen.1002584 PMID: 22479191
79. Mayers CM, Wadell J, McLean K, Venere M, Malik M, Shibata T, et al. The Rho guanine nucleotide exchange factor AKAP13 (BRX) is essential for cardiac development in mice. J Biol Chem. 2010; 285: 12344-12354. doi: 10.1074/jbc.M110.106856 PMID: 20139090
80. Kwak EL, Bang YJ, Camidge DR, Shaw AT, Solomon B, Maki RG, et al. Anaplastic lymphoma kinase inhibition in non-small-cell lung cancer. N Engl J Med. 2010; 363: 1693-1703. doi: 10.1056/NEJMoa1006448 PMID: 20979469
81. Koivunen JP, Mermel C, Zejnullahu K, Murphy C, Lifshits E, Holmes AJ, et al. EML4-ALK fusion gene and efficacy of an ALK kinase inhibitor in lung cancer. Clin Cancer Res. 2008; 14: 4275-4283. doi: 10. 1158/1078-0432.CCR-08-0168 PMID: 18594010
82. Carniel E, Taylor MR, Sinagra G, Di LA, Ku L, Fain PR, et al. Alpha-myosin heavy chain: A sarcomeric gene associated with dilated and hypertrophic phenotypes of cardiomyopathy. Circulation. 2005; 112: 54-59. PMID: 15998695
83. Niimura H, Patton KK, McKenna WJ, Soults J, Maron BJ, Seidman JG, et al. Sarcomere protein gene mutations in hypertrophic cardiomyopathy of the elderly. Circulation. 2002; 105: 446-451. PMID: 11815426
84. Ching YH, Ghosh TK, Cross SJ, Packham EA, Honeyman L, Loughna S, et al. Mutation in myosin heavy chain 6 causes atrial septal defect. Nat Genet. 2005; 37: 423-428. PMID: 15735645
85. Kamisago M, Sharma SD, DePalma SR, Solomon S, Sharma P, McDonough B, et al. Mutations in sarcomere protein genes as a cause of dilated cardiomyopathy. N Engl J Med. 2000; 343: 1688-1696. PMID: 11106718
86. Shih YH, Zhang Y, Ding Y, Ross CA, Li H, Olson TM, et al. Cardiac transcriptome and dilated cardiomyopathy genes in zebrafish. Circ Cardiovasc Genet. 2015; 8: 261-269. doi: 10.1161/CIRCGENETICS. 114.000702 PMID: 25583992
87. Osio A, Tan L, Chen SN, Lombardi R, Nagueh SF, Shete S, et al. Myozenin 2 is a novel gene for human hypertrophic cardiomyopathy. Circ Res. 2007; 100: 766-768. PMID: 17347475
88. Watkins H, McKenna WJ, Thierfelder L, Suk HJ, Anan R, O'Donoghue A, et al. Mutations in the genes for cardiac troponin T and alpha-tropomyosin in hypertrophic cardiomyopathy. N Engl J Med. 1995; 20; 332: 1058-1064. PMID: 7898523
89. Tabibiazar R, Wagner RA, Liao A, Quertermous T. Transcriptional profiling of the heart reveals chamber-specific gene expression patterns. Circ Res. 2003; 93: 1193-1201. PMID: 14576202
90. Sakuntabhai A, Ruiz-Perez V, Carter S, Jacobsen N, Burge S, Monk S, et al. Mutations in ATP2A2, encoding a Ca2+ pump, cause Darier disease. Nat Genet. 1999; 21: 271-277. PMID: 10080178
91. Ji Y, Lalli MJ, Babu GJ, Xu Y, Kirkpatrick DL, Liu LH, et al. Disruption of a single copy of the SERCA2 gene results in altered Ca2+ homeostasis and cardiomyocyte function. J Biol Chem. 2000; 275: 38073-38080. PMID: 10970890
92. Wang Z, Gerstein M, Snyder M. RNA-Seq: A revolutionary tool for transcriptomics. Nat Rev Genet. 2009; 10: 57-63. doi: 10.1038/nrg2484 PMID: 19015660