Alphavirus capsid proteins self-assemble into core-like particles in insect cells: A promising platform for nanoparticle vaccine development

Biotechnology Journal

Volumn 11, Issue 2, 2016, Pages 266-273

  Hikke, Mia C ^a   Geertsema, Corinne ^a   Wu, Vincen ^a   Metz, Stefan W ^a   van Lent, Jan W ^a   Vlak, Just M ^a   Pijlman, Gorben P ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Alphavirus; Baculovirus expression; Core like particle; Protein carrier; Subunit vaccine

Indexed keywords

CELLS; CYTOLOGY; EPITOPES; GLYCOPROTEINS; PROTEINS; VACCINES; VIRUSES;

ALPHAVIRUS; BACULOVIRUS; BIOTECHNOLOGICAL APPROACHES; PROTEIN CARRIER; RECOMBINANT BACULOVIRUS; SELF-ASSEMBLING PROPERTIES; SUBUNIT VACCINE; SURFACE GLYCOPROTEINS;

RECOMBINANT PROTEINS;

BACULOVIRUS VECTOR; CAPSID PROTEIN; EPITOPE; GLYCOPROTEIN E2; NANOPARTICLE; HYBRID PROTEIN; VIRUS ENVELOPE PROTEIN; VIRUS LIKE PARTICLE VACCINE;

ALPHAVIRUS; ANTIGEN BINDING; ARTICLE; CELL NUCLEUS INCLUSION BODY; CENTRIFUGATION; CHIKUNGUNYA VIRUS; CONTROLLED STUDY; CORE LIKE PARTICLE; ELECTRON MICROSCOPY; INSECT CELL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SALMONID ALPHAVIRUS; ULTRASOUND; VIRUS LIKE AGENT; VIRUS NUCLEOCAPSID; WESTERN BLOTTING; ANIMAL; BACULOVIRIDAE; BIOSYNTHESIS; CELL NUCLEUS; DRUG DESIGN; GENETICS; IMMUNOLOGY; METABOLISM; SF9 CELL LINE; VIRUS ASSEMBLY;

ALPHAVIRUS; ANIMALS; BACULOVIRIDAE; CAPSID PROTEINS; CELL NUCLEUS; DRUG DESIGN; EPITOPES; RECOMBINANT FUSION PROTEINS; SF9 CELLS; VACCINES, VIRUS-LIKE PARTICLE; VIRAL ENVELOPE PROTEINS; VIRUS ASSEMBLY;

EID: 84957432285     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201500147     Document Type: Article

References (36)

1. Paul, F. H., Lisa, J. R., Rosheila, D., Melinda, S. et al., Outbreak of chikungunya virus infection, Vanimo, Papua New Guinea. Emerg. Infect. Dis. 2013, 19, 1535.
2. Suhrbier, A., Jaffar-Bandjee, M.-C., Gasque, P., Arthritogenic alphaviruses - an overview. Nat. Rev. Rheumatol. 2012, 8, 420-429.
3. Rezza, G., Nicoletti, L., Angelini, R., Romi, R. et al., Infection with chikungunya virus in Italy: An outbreak in a temperate region. Lancet 2007, 370, 1840-1846.
4. Marc, G., Valérie, C., Sébastien, P., Jean-Michel, T. et al., Chikungunya virus, southeastern France. Emerg. Infect. Dis. 2011, 17, 910.
5. Leparc-Goffart, I., Nougairede, A., Cassadou, S., Prat, C., de Lamballerie, X., Chikungunya in the Americas. Lancet 2014, 383, 514.
6. Hikke, M. C., Verest, M., Vlak, J. M., Pijlman, G. P., Salmonid alphavirus replication in mosquito cells: Towards a novel vaccine production system. Microb. Biotechnol. 2014, 7, 480-484.
7. Olsen, C. M., Pemula, A. K., Braaen, S., Sankaran, K., Rimstad, E., Salmonid alphavirus replicon is functional in fish, mammalian and insect cells and in vivo in shrimps (Litopenaeus vannamei). Vaccine 2013, 31, 5672-5679.
8. Jose, J., Snyder, J., Kuhn, R., A structural and functional perspective of alphavirus replication and assembly. Future Microbiol. 2009, 4, 837-856.
9. Kuhn, R. J. (Ed.), Togaviridae: The Viruses and their Replication, Lippincott-Raven Publishers, Eds Philadelphia 2007.
10. Metz, S. W., Geertsema, C., Martina, B. E., Andrade, P. et al., Functional processing and secretion of Chikungunya virus E1 and E2 glycoproteins in insect cells. Virol. J. 2011, 8, 353.
11. Metz, S. W., Martina, B. E., van den Doel, P., Geertsema, C. et al., Chikungunya virus-like particles are more immunogenic in a lethal AG129 mouse model compared to glycoprotein E1 or E2 subunits. Vaccine 2013, 31, 6092-6096.
12. Metz, S. W., Gardner, J., Geertsema, C., Le, T. T. et al., Effective chikungunya virus-like particle vaccine produced in insect cells. PLoS Negl. Trop. Dis. 2013, 7, e2124.
13. Akahata, W., Yang, Z.-Y., Andersen, H., Sun, S. et al., A virus-like particle vaccine for epidemic Chikungunya virus protects nonhuman primates against infection. Nat. Med. 2010, 16, 334-338.
14. Chang, L.-J., Dowd, K. A., Mendoza, F. H., Saunders, J. G. et al., Safety and tolerability of chikungunya virus-like particle vaccine in healthy adults: A phase 1 dose-escalation trial. Lancet 2014, 384, 2046-2052.
15. Metz, S. W., Feenstra, F., Villoing, S., van Hulten, M. C. et al., Low temperature-dependent salmonid alphavirus glycoprotein processing and recombinant virus-like particle formation. PLoS One 2011, 6, e25816.
16. Hikke, M. C., Braaen, S., Villoing, S., Hodneland, K. et al., Salmonid alphavirus glycoprotein E2 requires low temperature and E1 for virion formation and induction of protective immunity. Vaccine 2014, 32, 6206-6212.
17. Metz, S. W., Pijlman, G. P., Arbovirus vaccines; opportunities for the baculovirus-insect cell expression system. J. Invertebr. Pathol. 2011, 107, Supplement, S16-S30.
18. Lua, L. H. L., Connors, N. K., Sainsbury, F., Chuan, Y. P. et al., Bioengineering virus-like particles as vaccines. Biotechnol. Bioeng. 2014, 111, 425-440.
19. Garmashova, N., Gorchakov, R., Volkova, E., Paessler, S. et al., The Old World and New World alphaviruses use different virus-specific proteins for induction of transcriptional shutoff. Virol. J. 2007, 81, 2472-2484.
20. Fros, J. J., van der Maten, E., Vlak, J. M., Pijlman, G. P., The C-terminal domain of Chikungunya virus nsP2 independently governs viral RNA replication, cytopathicity, and Inhibition of interferon signaling. J. Virol. 2013, 87, 10394-10400.
21. Thomas, S., Rai, J., John, L., Schaefer, S. et al., Chikungunya virus capsid protein contains nuclear import and export signals. Virol. J. 2013, 10, 1-13.
22. Favre, D., Studer, E., Michel, M. R., Two nucleolar targeting signals present in the N-terminal part of Semliki Forest virus capsid protein. Arch. Virol. 1994, 137, 149-155.
23. Michel, M., Elgizoli, M., Dai, Y., Jakob, R. et al., Karyophilic properties of Semliki Forest virus nucleocapsid protein. J. Virol. 1990, 64, 5123-5131.
24. Jakob, R., Nucleolar accumulation of Semliki Forest virus nucleocapsid C protein: Influence of metabolic status, cytoskeleton and receptors. J. Med. Microbiol. 1994, 40, 389-392.
25. Hopkins, R. F., Esposito, D., A rapid method for titrating baculovirus stocks using the Sf-9 Easy Titer cell line. Biotechniques 2009, 47, 785-788.
26. Moriette, C., LeBerre, M., Boscher, S. K., Castric, J., Brémont, M., Characterization and mapping of monoclonal antibodies against the sleeping disease virus, an aquatic alphavirus. J. Gen. Virol. 2005, 86, 3119-3127.
27. Blissard, G. W., Rohrmann, G. F., Baculovirus diversity and molecular biology. Annu. Rev. Entomol. 1990, 35, 127-155.
28. Acheson, N. H., Tamm, I., Replication of semliki forest virus: An electron microscopic study. Virology 1967, 32, 128-143.
29. Hunt, A. R., Frederickson, S., Maruyama, T., Roehrig, J. T., Blair, C. D., The first human epitope map of the alphaviral E1 and E2 proteins reveals a new E2 epitope with significant virus neutralizing activity. PLoS Negl. Trop. Dis. 2010, 4, e739.
30. Porta, J., Jose, J., Roehrig, J. T., Blair, C. D. et al., Locking and blocking the viral landscape of an alphavirus with neutralizing antibodies. J. Virol. 2014, 88, 9616-9623.
31. Coffey, L. L., Vignuzzi, M., Host alternation of chikungunya virus increases fitness while restricting population diversity and adaptability to novel selective pressures. J. Virol. 2011, 85, 1025-1035.
32. Voss, J. E., Vaney, M.-C., Duquerroy, S., Vonrhein, C. et al., Glycoprotein organization of chikungunya virus particles revealed by X-ray crystallography. Nature 2010, 468, 709-712.
33. Snijders, A., Benaissa-Trouw, B. J., Oosterlaken, T. A. M., Puijk, W. C. et al., Identification of linear epitopes on Semliki Forest virus E2 membrane protein and their effectiveness as a synthetic peptide vaccine. J. Gen. Virol. 1991, 72, 557-565.
34. Karlsen, M., Andersen, L., Blindheim, S. H., Rimstad, E., Nylund, A., A naturally occurring substitution in the E2 protein of Salmonid alphavirus subtype 3 changes viral fitness. Virus. Res. 2015, 196, 79-86.
35. Roy, P., Noad, R., Virus-like particles as a vaccine delivery system: Myths and facts, in: Guzmán, C., Feuerstein, G. (Eds.), Pharmaceutical Biotechnology, Vol. 655, Springer New York 2009, pp. 145-158.
36. Kam, Y.-W., Lee, W. W. L., Simarmata, D., Le Grand, R. et al., Unique epitopes recognized by antibodies induced in chikungunya virus-infected non-human primates: Implications for the study of immunopathology and vaccine development. PLoS One 2014, 9, e95647.