On-site detection of Xylella fastidiosa in host plants and in "spy insects" using the real-time loop-mediated isothermal amplification method

Phytopathologia Mediterranea

Volumn 54, Issue 3, 2015, Pages 488-496

  Yaseen, Thaer ^a   Drago, Sandro ^b   Valentini, Franco ^a   Elbeaino, Toufic ^a   Stampone, Giuseppe ^b   Digiaro, Michele ^a   D'Onghia, Anna Maria ^a  

^a ISTITUTO AGRONOMICO MEDITERRANEO   (Italy)

^b Bionat Italia Srl   (Italy)

Author keywords

Olive quick decline syndrome (OQDS); Philaenus spumarius; Real time LAMP

Indexed keywords

EID: 84956661131     PISSN: 00319465     EISSN: 15932095     Source Type: Journal    
DOI: 10.14601/Phytopathol_Mediterr-15250     Document Type: Article

References (21)

1. Cariddi C., M. Saponari, D. Boscia, A. De Stradis, G. Loconsole, F. Nigro, F. Porcelli, O. Potere and G.P. Martelli, 2014. Isolation of a Xylella fastidiosa strain infecting olive and oleander in Apulia, Italy. Journal of Plant Pathology 96(2), 425-429.
2. Chatterjee S., R.P.P. Almeida and S. Lindow, 2008. Living in two worlds: The plant and insect lifestyles of Xylella fastidiosa. Annual Review of Phytopathology 46, 243-271.
3. Djelouah K., D. Frasheri, F. Valentini, A.M. D'Onghia and M. Digiaro, 2014. Direct tissue blot immunoassay for detec- tion of Xylella fastidiosa in olive trees. Phytopathologia Mediterranea 53(3), 559-564.
4. Elbeaino T., T. Yaseen, F. Valentini, I. E. Ben Moussa, V. Mazzoni and A.M. D'Onghia, 2014. Identification of three potential insect vectors of Xylella fastidiosa in southern Italy. Phytopathologia Mediterranea 53(2), 328-332.
5. Francis M., H. Lin, J. Cabrera-La Rosa, H. Doddapaneni and E.L. Civerolo, 2006. Genome-based PCR primers for specific and sensitive detection and quantification of Xylella fastidiosa. European Journal of Plant Pathology 115(2), 203- 213.
6. Harper S., L. Ward and G. Clover, 2010. Development of LAMP and real-time PCR methods for the rapid detection of Xylella fastidiosa for quarantine and field applications. Phytopathology 100(12), 1282-1288.
7. Huang Q., 2009. Specific detection and identification of Xylella fastidiosa strains causing Oleander leaf scorch using polymerase chain reaction. Current Microbiology 58(4), 393-398.
8. Huang Q., J. Bentz and J. L. Sherald, 2006. Fast, easy and efficient DNA extraction and one-step polymerase chain reaction for the detection of Xylella fastidiosa in potential insect vectors. Journal of Plant Pathology 88(1), 77-81.
9. Janse J. and B. Kokoskova, 2009. Indirect immunofluorescence microscopy for the detection and identification of plant pathogenic bacteria (in particular for Ralstonia solanacearum). Methods in Molecular Biology 508, 89-99.
10. Kogovšek P., J. Hodgetts, J. Hall, N. Prezelj, P. Nikolic, N. Mehle, R. Lenarcic, A. Rotter, M. Dickinson, N. Boonham, M. Dermastia and M. Ravnikar, 2014. LAMP assay and rapid sample preparation method for on-site detection of flavescence dorée phytoplasma in grapevine. Plant Pathology, 286-296.
11. Lin H. and M.A. Walker, 1997. Extracting DNA from cambium tissue for analysis of grape rootstocks. Hortscience 32(7), 1264-1266.
12. Loconsole G., O. Potere, D. Boscia, G. Altamura, K. Djelouah, T. Elbeaino, D. Frasheri, D. Lorusso, F. Palmisano, P. Pollastro, M.R. Silletti, N. Trisciuzzi, F. Valentini, V. Savino and M. Saponari, 2014. Detection of Xylella fastidiosa in olive trees by molecular and serological methods. Journal of Plant Pathology 96(1), 7-14.
13. Minsavage G.V., C.M. Thompson, D.L. Hopkins, R.M.V.B.C. Leite and R.E. Stall, 1994. Development of a Polymerase Chain-Reaction Protocol for Detection of Xylella-Fastidiosa in Plant-Tissue. Phytopathology 84(5), 456-461.
14. Notomi T., H. Okayama, H. Masubuchi, T. Yonekawa, K. Watanabe, N. Amino and T. Hase, 2000. Loop-mediated isothermal amplification of DNA. Nucleic Acids Research 28(12), e63.
15. Pooler M.R. and J.S. Hartung, 1995. Genetic-relationships among strains of Xylella fastidiosa from RAPD-PCR data. Current Microbiology 31(2), 134-137.
16. Purcell A.H., 1997. Xylella fastidiosa, a regional problem or global threat? Journal of Plant Pathology 79(2), 99-105.
17. Purcell A.H. and A. Finlay, 1979. Evidence for noncirculative transmission of Pierce's disease bacterium by sharpshooter leafhoppers. Phytopathology 69, 393-395.
18. Redak R.A., A.H. Purcell, J.R. Lopes, M.J. Blua, R.F. Mizell 3rd and P.C. Andersen, 2004. The biology of xylem fluidfeeding insect vectors of Xylella fastidiosa and their relation to disease epidemiology. Annual Review of Entomology 49, 243-270.
19. Rodrigues J.L.M., M.E. Silva-Stenico, J.E. Gomes, J.R.S. Lopes and S.M. Tsai, 2003. Detection and diversity assessment of Xylella fastidiosa in field-collected plant and insect samples by using 16S rRNA and gyrB sequences. Applied and Environmental Microbiology 69(7), 4249-4255.
20. Saponari M., D. Boscia, F. Nigro and G.P. Martelli, 2013. Identification of DNA sequences related to Xylella fastidiosa in oleander, almond and olive trees exhibiting leaf scorch symptoms in Apulia (Southern Italy). Journal of Plant Pathology 95(3), 668-668.
21. Schaad N.W., D. Opgenorth and P. Gaush, 2002. Real-time polymerase chain reaction for one-hour on-site diagnosis of Pierce's disease of grape in early season asymptomatic vines. Phytopathology 92(7), 721-728.