Efficient expression and purification of porcine circovirus type 2 virus-like particles in Escherichia coli

Journal of Biotechnology

Volumn 220, Issue , 2016, Pages 78-85

  Wu, Pei Ching ^a   Chen, Tzu Yu ^a   Chi, Jiun Ni ^a   Chien, Maw Sheng ^a   Huang, Chienjin ^a  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

Author keywords

PCV2 capsid protein; Size exclusion chromatography; Vaccine; Virus like particles

Indexed keywords

CHROMATOGRAPHY; COLUMN CHROMATOGRAPHY; DISEASE CONTROL; ESCHERICHIA COLI; GENE EXPRESSION; MAMMALS; NUCLEIC ACIDS; PROTEINS; PURIFICATION; SIZE EXCLUSION CHROMATOGRAPHY; VACCINES; VIRUSES;

BACTERIAL CULTURES; CAPSID PROTEINS; EXPRESSION SYSTEM; HIGH LEVEL EXPRESSION; IMMUNE RESPONSE; LARGE SCALE PRODUCTIONS; RECOMBINANT PLASMID; VIRUS-LIKE PARTICLES;

RECOMBINANT PROTEINS;

CAPSID PROTEIN; GAMMA INTERFERON; IMMUNOGLOBULIN G; NEUTRALIZING ANTIBODY; RECOMBINANT PORCINE CIRCOVIRUS TYPE 2 CAPSID PROTEIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VIRUS VACCINE; SUBUNIT VACCINE; VIRUS ANTIBODY; VIRUS LIKE PARTICLE VACCINE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY RESPONSE; ANTIBODY TITER; ARTICLE; BACTERIUM CULTURE; BACULOVIRUS EXPRESSION SYSTEM; CELLULAR IMMUNITY; CIRCOVIRIDAE INFECTION; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; FEMALE; FRACTIONATION; HUMORAL IMMUNITY; IMMUNE RESPONSE; IMMUNIZATION; IMMUNOGENICITY; LARGE SCALE PRODUCTION; MOUSE; NONHUMAN; ORF2 GENE; PORCINE CIRCOVIRUS 2; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RECOMBINANT PLASMID; SECRETORY CELL; SERODIAGNOSIS; SIZE EXCLUSION CHROMATOGRAPHY; SPLEEN CELL; TRANSMISSION ELECTRON MICROSCOPY; VIRUS CAPSID; VIRUS GENE; VIRUS LIKE AGENT; ANIMAL; ANTIBODY PRODUCTION; BAGG ALBINO MOUSE; BIOSYNTHESIS; BLOOD; CHEMISTRY; CIRCOVIRUS; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME LINKED IMMUNOSPOT ASSAY; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PIG; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCEDURES; SEQUENCE ALIGNMENT; SWINE DISEASES; VIRION; VIROLOGY; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, VIRAL; ANTIBODY FORMATION; BLOTTING, WESTERN; CAPSID PROTEINS; CHROMATOGRAPHY, GEL; CIRCOVIRIDAE INFECTIONS; CIRCOVIRUS; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; ENZYME-LINKED IMMUNOSPOT ASSAY; ESCHERICHIA COLI; FEMALE; MICE; MICE, INBRED BALB C; MICROSCOPY, ELECTRON, TRANSMISSION; MOLECULAR SEQUENCE DATA; NEUTRALIZATION TESTS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SWINE; SWINE DISEASES; VACCINES, SUBUNIT; VACCINES, VIRUS-LIKE PARTICLE; VIRION;

EID: 84955512611     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2016.01.017     Document Type: Article

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