Synergistic Effect of Simple Sugars and Carboxymethyl Cellulose on the Production of a Cellulolytic Cocktail from Bacillus sp. AR03 and Enzyme Activity Characterization

Applied Biochemistry and Biotechnology

Volumn 179, Issue 1, 2016, Pages 16-32

  Manfredi, Adriana P ^a   Pisa, José H ^a   Valdeón, Daniel H ^b   Perotti, Nora I ^a,b   Martínez, María A ^a,b  

^a PLANTA PILOTO DE PROCESOS INDUSTRIALES MICROBIOLÓGICOS PROIMI CONICET   (Argentina)

^b UNIVERSIDAD NACIONAL DE TUCUMÁN   (Argentina)

Author keywords

Bacillus sp. AR03; Biorefinery; Endoglucanase production; Enzyme activity; Factorial design

Indexed keywords

BACTERIOLOGY; CELLULOSE; ENZYMES; MANGANESE; PH EFFECTS; REFINING; SUGARS;

BACILLUS SP; BIOREFINERIES; CARBOXYMETHYL CELLULOSE; ENDOGLUCANASES; FACTORIAL DESIGN; FERMENTATION PERIODS; MONO- AND DISACCHARIDES; STIRRED TANK REACTORS;

ENZYME ACTIVITY;

BACTERIAL RNA; BARIUM ION; CALCIUM ION; CARBOHYDRATE; CARBOXYMETHYLCELLULOSE; CASEIN; CELLULASE; DISACCHARIDE; EDETIC ACID; FRUCTOSE; GLUCAN SYNTHASE; GLUCOSE; IRON; LACTOSE; MALTOSE; MANGANESE; MANGANESE DERIVATIVE; MONOSACCHARIDE; PEPTONE; POLYSORBATE 80; POTASSIUM ION; RNA 16S; SODIUM CHLORIDE; SODIUM ION; SUCROSE; ZINC ION; CELLULOSE;

AERATION; ARTICLE; BACILLUS; BACILLUS SUBTILIS SUBSP INAQUOSORUM; BACILLUS SUBTILIS SUBSP SUBTILIS; BACILLUS TEQUILENSI; BACTERIAL STRAIN; CARBON SOURCE; CENTRIFUGATION; CONTROLLED STUDY; ENZYMATIC ASSAY; ENZYME ACTIVITY; ENZYME SYNTHESIS; FACTORIAL DESIGN; FERMENTATION; GENE SEQUENCE; NATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS; NONHUMAN; PH; SILVER STAINING; STIRRED REACTOR; TEMPERATURE; THERMOSTABILITY; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY;

BACILLUS; CARBOHYDRATES; CARBOXYMETHYLCELLULOSE SODIUM; CELLULASE; CELLULOSE; FERMENTATION; MONOSACCHARIDES; TEMPERATURE;

EID: 84955265528     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-015-1976-5     Document Type: Article

References (33)

1. Linares-Pastén, J. A., Andersson, M., & Karlsson, E. N. (2014). Thermostable glycoside hydrolases in biorefinery technologies. Current Biotechnology, 3, 26–44. doi:10.2174/22115501113026660041.
2. Balasubramanian, N., & Simões, N. (2014). Bacillus pumilus S124A carboxymethyl cellulase; a thermo stable enzyme with a wide substrate spectrum utility. International Journal of Biological Macromolecules, 67, 132–139. doi:10.1016/j.ijbiomac.2014.03.014.
3. Rastogi, G., Bhalla, A., Adhikari, A., Bischoff, K. M., Hughes, S. R., Christopher, L. P., & Sani, R. K. (2010). Characterization of thermostable cellulases produced by Bacillus and Geobacillus strains. Bioresource Technology, 101, 8798–8806. doi:10.1016/j.biortech.2010.06.001.
4. Wang, W., Yan, L., Cui, Z., Gao, Y., Wang, Y., & Jing, R. (2011). Characterization of a microbial consortium capable of degrading lignocellulose. Bioresource Technology, 102, 9321–9324. doi:10.1016/j.biortech.2011.07.065.
5. Tarayre, C., Brognaux, A., Bauwens, J., Brasseur, C., Mattéotti, C., Millet, C., Destain, J., Vandenbol, M., Portetelle, D., De Pauw, E., Eric, H., Francis, F., & Thonart, P. (2014). Isolation of amylolytic, xylanolytic, and cellulolytic microorganisms extracted from the gut of the termite Reticulitermes santonensis by means of a micro-aerobic atmosphere. World Journal of Microbiology and Biotechnology, 30, 1655–1660. doi:10.1007/s11274-013-1585-9.
6. Sadhu, S., Saha, P., Mayilraj, S., & Maiti, T. K. (2011). Lactose-enhanced cellulase production by Microbacterium sp. isolated from fecal matter of zebra (Equus zebra). Current Microbiology, 62, 1050–1055. doi:10.1007/s00284-010-9816-x.
7. Sharma, A., & Satyanarayana, T. (2013). Comparative genomics of Bacillus species and its relevance in industrial microbiology. Genomics Insights, 6, 25–36. doi:10.4137/GEI.S12732.
8. Kazemi, A., Rasoul-Amini, S., Shahbazi, M., Safari, A., & Ghasemi, Y. (2014). Isolation, identification, and media optimization of high-level cellulase production by Bacillus sp. BCCS A3, in a fermentation system using response surface methodology. Preparative Biochemistry and Biotechnology, 44, 107–118. doi:10.1080/10826068.2013.792276.
9. Maki, M. L., Idrees, A., Leung, K. T., & Qin, W. (2012). Newly isolated and characterized bacteria with great application potential for decomposition of lignocellulosic biomass. Journal of Molecular Microbiology and Biotechnology, 22, 156–166. doi:10.1159/000341107.
10. Bano, S., Qader, S. A. U., Aman, A., Syed, M. N., & Durrani, K. (2013). High production of cellulose degrading endo-1,4-β-d-glucanase using bagasse as a substrate from Bacillus subtilis KIBGE HAS. Carbohydrate Polymers, 91, 300–304. doi:10.1016/j.carbpol.2012.08.022.
11. Nagar, S., Gupta, V. K., Kumar, D., Kumar, L., & Kuhad, R. C. (2010). Production and optimization of cellulase-free, alkali-stable xylanase by Bacillus pumilus SV-85S in submerged fermentation. Journal of Industrial Microbiology and Biotechnology, 37, 71–83. doi:10.1007/s10295-009-0650-8.
12. Lee, E.-J., Lee, B.-H., Kim, B.-K., & Lee, J.-W. (2013). Enhanced production of carboxymethyl cellulase of a marine microorganism, Bacillus subtilis subsp. subtilis A-53 in a pilot-scaled bioreactor by a recombinant Escherichia coli JM109/A-53 from rice bran. Molecular Biology Reports, 40, 3609–3621. doi:10.1007/s11033-012-2435-9.
13. Sadhu, S., Saha, P., Sen, S. K., Mayilraj, S., & Maiti, T. K. (2013). Production, purification and characterization of a novel thermotolerant endoglucanase (CMCase) from Bacillus strain isolated from cow dung. Springerplus, 2, 10. doi:10.1186/2193-1801-2-10.
14. Manfredi, A. P., Perotti, N. I., & Martínez, M. A. (2015). Cellulose degrading bacteria isolated from industrial samples and the gut of native insects from Northwest of Argentina. Journal of Basic Microbiology. doi:10.1002/jobm.201500269.
15. Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31, 426–428. doi:10.1021/ac60147a030.
16. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685. doi:10.1038/227680a0.
17. Teather, R. M., & Wood, P. J. (1982). Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Applied and Environmental Microbiology, 43, 777–780.
18. Patrick, J. E., & Kearns, D. B. (2009). Laboratory strains of Bacillus subtilis do not exhibit swarming motility. Journal of Bacteriology, 191(22), 7129–7133. doi:10.1128/JB.00905-09.
19. Lee, Y.-J., Kim, B.-K., Lee, B.-H., Jo, K.-I., Lee, N.-K., Chung, C.-H., Lee, Y.-C., & Lee, J.-W. (2008). Purification and characterization of cellulase produced by Bacillus amyoliquefaciens DL-3 utilizing rice hull. Bioresource Technology, 99, 378–386. doi:10.1016/j.biortech.2006.12.013.
20. Paudel, Y. P., & Qin, W. (2015). Characterization of novel cellulase-producing bacteria isolated from rotting wood samples. Applied Biochemistry and Biotechnology. doi:10.1007/s12010-015-1806-9.
21. Karaffa, L., Fekete, E., Gamauf, C., Szentirmai, A., Kubicek, C. P., & Seiboth, B. (2006). D-galactose induces cellulase gene expression in Hypocrea jecorina at low growth rates. Microbiology, 152, 1507–1514. doi:10.1099/mic.0.28719-0.
22. Gautam, S. P., Bundela, P. S., Pandey, A. K., Khan, J., Awasthi, M. K., & Sarsaiya, S. (2011). Optimization for the production of cellulase enzyme from municipal solid waste residue by two novel cellulolytic fungi. Biotechnology Research International, 2011, 810425. doi:10.4061/2011/810425.
23. Bajaj, B. K., Pangotra, H., Wani, M. A., Sharma, P., & Sharma, A. (2009). Partial purification and characterization of a highly thermostable and pH stable endoglucanase from a newly isolated Bacillus strain M-9. Indian Journal of Chemical Technology, 16, 382–387.
24. Zhang, G., Li, S., Xue, Y., Mao, L., & Ma, Y. (2012). Effects of salts on activity of halophilic cellulase with glucomannanase activity isolated from alkaliphilic and halophilic Bacillus sp. BG-CS10. Extremophiles, 16, 35–43. doi:10.1007/s00792-011-0403-2.
25. Singh, J., Batra, N., & Sobti, R. C. (2004). Purification and characterisation of alkaline cellulase produced by a novel isolate, Bacillus sphaericus JS1. Journal of Industrial Microbiology and Biotechnology, 31, 51–56. doi:10.1007/s10295-004-0114-0.
26. Shoseyov, O., & Doi, R. H. (1990). Essential 170-kDa subunit for degradation of crystalline cellulose by Clostridium cellulovorans cellulase. Proceedings of the National Academy of Sciences of the United States of America, 87, 2192–2195. doi:10.1073/pnas.87.6.2192.
27. Waeonukul, R., Kyu, K. L., Sakka, K., & Ratanakhanokchai, K. (2009). Isolation and characterization of a multienzyme complex (cellulosome) of the Paenibacillus curdlanolyticus B-6 grown on Avicel under aerobic conditions. Journal of Bioscience and Bioengineering, 107, 610–614. doi:10.1016/j.jbiosc.2009.01.010.
28. Gaur, R., & Tiwari, S. (2015). Isolation, production, purification and characterization of an organic-solvent-thermostable alkalophilic cellulase from Bacillus vallismortis RG-07. BMC Biotechnology, 15, 19. doi:10.1186/s12896-015-0129-9.
29. Franco-Cirigliano, M. N., Rezende Carvalho, R., Pires Gravina-Oliveira, M., Freitas Pereira, P. H., Pires Do Nascimento, R., Pinto da Silva Bon, E., Macrae, A., & Reed Rodrigues Coelho, R. (2013). Streptomyces misionensis PESB-25 produces a thermoacidophilic endoglucanase using sugarcane bagasse and corn steep liquor as the sole organic substrates. BioMed Research International, 2013, 1–9. doi:10.1155/2013/584207.
30. Balasubramanian, N., Toubarro, D., Teixeira, M., & Simõs, N. (2012). Purification and biochemical characterization of a novel thermo-stable carboxymethyl cellulase from Azorean isolate Bacillus mycoides S122C. Applied Biochemistry and Biotechnology, 168, 2191–2204. doi:10.1007/s12010-012-9929-8.
31. Vijayaraghavan, P., & Vincent, S. G. P. (2012). Purification and characterization of carboxymethyl cellulase from Bacillus sp. isolated from a paddy field. Polish Journal of Microbiology, 61, 51–55.
32. Mamo, G., Hatti-Kaul, R., & Mattiasson, B. (2006). A thermostable alkaline active endo-β-1-4-xylanase from Bacillus halodurans S7: purification and characterization. Enzyme and Microbial Technology, 39, 1492–1498. doi:10.1016/j.enzmictec.2006.03.040.
33. Sinha, R., & Khare, S. K. (2014). Protective role of salt in catalysis and maintaining structure of halophilic proteins against denaturation. Frontiers in Microbiology, 5, 1–6. doi:10.3389/fmicb.2014.00165.