Purification and characterization of carboxymethyl cellulase from Bacillus sp. isolated from a paddy field

Polish Journal of Microbiology

Volumn 61, Issue 1, 2012, Pages 51-55

  Vijayaraghavan, Ponuswamy ^a   Vincent, S G Prakash ^b  

^a MANONMANIAM SUNDARANAR UNIVERSITY   (India)

^b Centre for Marine Science and Technology   (India)

Author keywords

Bacillus sp.; Biological degradation; Carboxymethyl cellulase; Cellulase purification; Paddy field

Indexed keywords

AMMONIUM SULFATE; CALCIUM ION; CARBOXYMETHYLCELLULOSE; COPPER ION; MAGNESIUM ION; MERCURY; SEPHADEX; SODIUM ION; ZINC ION;

ARTICLE; BACILLUS; BACTERIUM ISOLATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; MOLECULAR WEIGHT; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; TEMPERATURE;

BACILLUS SP.; BACTERIA (MICROORGANISMS);

EID: 84860466854     PISSN: 17331331     EISSN: None     Source Type: Journal    
DOI: 10.33073/pjm-2012-006     Document Type: Article

References (19)

1. Ando S., H. Ishida, Y. Kosugi and K. Ishikawa. 2002. Hyperthermostable endoglucanase from Pyrococcus horikoshii. Appl. Environ. Microbiol. 68: 430-433.
2. Camassola M. and A.J.P. Dillon. 2007. Production of cellulases and hemicellulases by Penicillium echinulatum grown on pretreated sugarcane bagasse and wheat bran in solid-state fermentation. J. Appl. Microbiol. 103: 2196-2204.
3. Dhillon N., S. Chhibber, M. Sexena, S. Pajni and D.V. Vadehra. 1985. A constitutive endoglucanase (CMCase) from Bacillus licheniformis-1. Biotechnol. Lett. 7: 695-697.
4. Henriette C., S. Zinebi, M.F. Aumaitre and E. Petitdemange. 1993. Protease and lipase production by strain of Serratia marcescens. J. Industrial. Microbiol. 12: 129-135.
5. Jones D. and M.D. Collins. 1984. Irregular, nonsporeforming Grampositive rods. pp. 1261-1434. In: Bergey's Manual of Systematic Bacteriology. (eds). P.H.A. Sneath, Baltimore, MD: Williams and Wilkins.
6. Kasana R.C., R. Salwan, H. Dhar, S. Dutt, A. Gulati. 2008. A Rapid and Easy Method for the Detection of Microbial Cellulases on Agar Plates Using Gram's Iodine. Curr. Microbiol. 57: 503-507.
7. Kotchoni S.O., E.W. Gachomo, B.O. Omafuvbe and O.O. Shonukan. 2006. Purification and Biochemical Characterization of Carboxymethyl Cellulase (CMCase) from a Catabolite Repression Insensitive Mutant of Bacillus pumilus. Int. J. Agri. Biol. 8: 286-292.
8. Laemmli U.K. 1970. "Cleavage of structural proteins during the assembly of the head of bacteriophage T4". Nature 227: 680-685.
9. Lee K.D, J. Kim and H. Kim. 1996. Isolation and Characterization of Bacillus sp. KD1014 Producing Carboxymethyl-Cellulase. J. Microbiol. 34: 305-310.
10. Lee Y.J., B.K. Kim, B.H. Lee, K.I. Jo, N.K. Lee, C.H. Chung, Y.C. Lee and J.W. Lee. 2008. Purification and characterization of cellulase produced by Bacillus amyoliquefaciens DL-3 utilizing rice hull. Bioresour. Technol. 99: 378-386.
11. Lowry O.H., N.J. Rosenbrough A.L. Fare and R.J. Randal. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 194: 265-275.
12. Lynd L.R., P.J. Weimer, W.H. van Zyl and I.S. Pretorius. 2002. Microbial cellulose utilization: fundamentals and biotechnology. Microbiol. Mol. Biol. Rev. 66: 506-577.
13. Mawadza C., R. Hatti-Kaul, R. Zvauya and B. Mattiasson. 2000. Purification and characterization of cellulases produced by two Bacillus strains. J. Biotechnol. 83: 177-187.
14. Nelson. 1944. A photometric adaption of the somogyi method for the determination of glucose. J. Biol. Chem. 153: 375-380.
15. Nishida Y., K.I. Suzuki, Y. Kumagai, H. Tanaka, A. Inoue and T. Ojima. 2007. Isolation and primary structure of a cellulase from the Japanese sea urchin Strongylocentrotus nudus. Biochimie 89: 1002-1011.
16. Odeniyi1, O.A., A.A. Onilude and M.A. Ayodele. 2009. Production characteristics and properties of cellulase/polygalacturonase by a Bacillus coagulans strain from a fermenting palm-fruit industrial residue. Afr. J. Microbiol. Res. 3: 407-417.
17. Perez J., J. Munoz-Dorado, T. Rubia and J. Martinez. 2002. "Biodegradation and biological treatments of cellulose, hemicellulose and lignin:an overview." Int. Microbiol. 5: 53-63.
18. Singh V.K. and A. Kumar. 1998. Production and purification of an extracellular cellulase from Bacillus brevis VS-1. Biochem. Mol. Biol. Int. 45: 443-452.
19. Yin L.J., H.H. Lin and Z.R. Xiao. 2010 Purification and Characterization of a cellulase from Bacillus subtilis YJ1. J. Marine Sci. Tech. 18: 466-471.