A fluorescence-based screening assay for identification of hepatitis C virus NS3 helicase inhibitors and characterization of their inhibitory mechanism

Methods in Molecular Biology

Volumn 1259, Issue , 2015, Pages 211-228

  Furuta, Atsushi ^a   Salam, Kazi Abdus ^a   Tani, Hidenori ^a   Tsuneda, Satoshi ^a   Sekiguchi, Yuji ^a   Akimitsu, Nobuyoshi ^a   Noda, Naohiro ^a  

^a NONE

Author keywords

Fluorescence assay; Fluorescence resonance energy transfer (FRET); Hepatitis C virus; High throughput screening assay; Inhibitor; Inhibitory mechanism; NS3 helicase; Radioactive assay

Indexed keywords

ADENOSINE TRIPHOSPHATASE; DOUBLE STRANDED RNA; HELICASE; NONSTRUCTURAL PROTEIN 3; NS3 PROTEIN, HEPATITIS C VIRUS; VIRUS PROTEIN;

ARTICLE; CATALYSIS; ENZYME ACTIVITY; ENZYME ASSAY; FLUORESCENCE ANALYSIS; FLUORESCENCE RESONANCE ENERGY TRANSFER; HEPATITIS C VIRUS; HIGH THROUGHPUT SCREENING; IC50; NONHUMAN; PRIORITY JOURNAL; RADIOACTIVITY; RNA BINDING; BIOASSAY; CHEMISTRY; ENZYMOLOGY; HEPACIVIRUS; METABOLISM; PROCEDURES;

HEPATITIS C VIRUS;

BIOLOGICAL ASSAY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HEPACIVIRUS; VIRAL NONSTRUCTURAL PROTEINS;

EID: 84954592489     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2214-7_14     Document Type: Article

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