High-throughput screening assay of hepatitis C virus helicase inhibitors using fluorescence-quenching phenomenon

Biochemical and Biophysical Research Communications

Volumn 379, Issue 4, 2009, Pages 1054-1059

  Tani, Hidenori ^a,b   Akimitsu, Nobuyoshi ^c   Fujita, Osamu ^a,b   Matsuda, Yasuyoshi ^a,b   Miyata, Ryo ^b   Tsuneda, Satoshi ^a   Igarashi, Masayuki ^d   Sekiguchi, Yuji ^b   Noda, Naohiro ^a,b  

^a WASEDA UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

^d INSTITUTE OF MICROBIAL CHEMISTRY   (Japan)

Author keywords

BODIPY FL; Fluorescence quenching; Hepatitis C virus; NS3 helicase; Photoinduced electron transfer

Indexed keywords

ADENOSINE 5' O (3 THIOTRIPHOSPHATE); DOUBLE STRANDED DNA; FLUORESCENT DYE; GUANINE; HELICASE; NONSTRUCTURAL PROTEIN 3; POTASSIUM CHLORIDE; SODIUM CHLORIDE;

ARTICLE; CONTROLLED STUDY; ELECTRON TRANSPORT; ENZYME ACTIVITY; HEPATITIS C VIRUS; METHODOLOGY; NONHUMAN; NONPHOTOCHEMICAL QUENCHING; PRIORITY JOURNAL; QUANTITATIVE ASSAY; STREPTOMYCES;

ANTIVIRAL AGENTS; DNA HELICASES; DRUG EVALUATION, PRECLINICAL; ENZYME INHIBITORS; FLUORESCENCE; FLUORESCENT DYES; HEPACIVIRUS; VIRAL NONSTRUCTURAL PROTEINS;

HEPATITIS C VIRUS;

EID: 58949094336     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.01.020     Document Type: Article

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