Preparation of cultured cells using high-pressure freezing and freeze substitution for subsequent 2D or 3D visualization in the transmission electron microscope

Methods in Molecular Biology

Volumn 1282, Issue , 2015, Pages 271-282

  Hawes, Philippa C ^a  

^a NONE

Author keywords

Electron tomography; Freeze substitution; High pressure freezing; Sapphire discs; Transmission electron microscopy

Indexed keywords

ICE; RESIN;

ANIMAL CELL; ARTICLE; ARTIFACT; CELL CULTURE; CHEMICAL STRESS; CRYOPRESERVATION; CYTOARCHITECTURE; ELECTRON TOMOGRAPHY; FREEZE DRYING; FREEZING; HIGH PRESSURE FREEZING; IMAGING AND DISPLAY; MECHANICAL STRESS; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; QUALITY CONTROL; TEMPERATURE; THREE DIMENSIONAL IMAGING; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE; ANIMAL; CELL NUCLEUS; CHLOROCEBUS AETHIOPS; CORONAVIRIDAE; HOST PATHOGEN INTERACTION; PHYSIOLOGY; VERO CELL LINE;

ANIMALS; CELL NUCLEUS; CERCOPITHECUS AETHIOPS; CORONAVIRUS; CRYOPRESERVATION; FREEZE SUBSTITUTION; FREEZING; HOST-PATHOGEN INTERACTIONS; MICROSCOPY, ELECTRON, TRANSMISSION; VERO CELLS;

EID: 84954582567     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-2438-7_23     Document Type: Article

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