Genetic and functional characterization of an extracellular modular GH6 endo-β-1,4-glucanase from an earthworm symbiont, Cellulosimicrobium funkei HY-13

Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

Volumn 109, Issue 1, 2016, Pages 1-12

  Kim, Do Young ^a   Lee, Min Ji ^a   Cho, Han Young ^a   Lee, Jong Suk ^b   Lee, Mi Hwa ^c   Chung, Chung Wook ^d   Shin, Dong Ha ^e   Rhee, Young Ha ^f   Son, Kwang Hee ^a   Park, Ho Yong ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b Gyeonggi Institute of Science and Technology Promotion   (South Korea)

^c Korea Food Research Institute   (South Korea)

^d Andong National University   (South Korea)

^e Hanson Biotech Co Ltd   (South Korea)

^f Chungnam National University   (South Korea)

Author keywords

Cellulosimicrobium funkei HY 13; Earthworm symbiotic bacterium; Endo 1,4 glucanase; GH family 6

Indexed keywords

CELLULASE; PROTEIN CELL; UNCLASSIFIED DRUG; BETA GLUCAN; BETA-1,3-D-GLUCAN; CARBOXYMETHYLCELLULOSE; CELLOBIOSE; CHITIN; LIGNIN; MANNAN; XYLAN;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENE; BACTERIUM; CARBOXY TERMINAL SEQUENCE; CELL GENE; CELLULOSIMICROBIUM FUNKEI; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; HYDROLYSIS; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; AMINO ACID SEQUENCE; ANIMAL; CELLULOMONAS; CHEMISTRY; ENZYME ACTIVATION; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; MOLECULAR GENETICS; OLIGOCHAETA; PH;

AMINO ACID SEQUENCE; ANIMALS; BETA-GLUCANS; CARBOXYMETHYLCELLULOSE SODIUM; CELLOBIOSE; CELLULASE; CELLULOMONAS; CHITIN; ENZYME ACTIVATION; ENZYME STABILITY; HYDROGEN-ION CONCENTRATION; LIGNIN; MANNANS; MOLECULAR SEQUENCE DATA; OLIGOCHAETA; XYLANS;

EID: 84954395806     PISSN: 00036072     EISSN: 15729699     Source Type: Journal    
DOI: 10.1007/s10482-015-0604-2     Document Type: Article

References (25)

1. Cazemier AE, Verdoes JC, Op den Camp HJM, Hackstein JHP, van Ooyen AJJ (1999) A β-1,4-endoglucanase-encoding gene from Cellulomonas pachnodae. Appl Microbiol Biotechnol 52:232–239
2. Chung SH, Choi GG, Kim HW, Rhee YH (2001) Effect of levulinic acid on the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Ralstonia eutropha KHB-8862. J Microbiol 39:79–82
3. Dimarogona M, Topakas E, Christakopoulos P (2013) Recalcitrant polysaccharide degradation by novel oxidative biocatalysts. Appl Microbiol Biotechnol 97:8455–8465
4. Ding SY, Liu YS, Zeng Y, Himmel ME, Baker JO, Bayer EA (2012) How does plant cell wall nanoscale architecture correlate with enzymatic digestibility? Science 338:1055–1060
5. Kim DY, Han MK, Lee JS, Oh H-W, Park D-S, Shin D-H, Bae KS, Son K-H, Park H-Y (2009a) Isolation and characterization of a cellulase-free endo-β-1,4-xylanase produced by an invertebrate-symbiotic bacterium, Cellulosimicrobium sp. HY-13. Proc Biochem 44:1055–1059
6. Kim DY, Han MK, Park D-S, Lee JS, Oh H-W, Shin D-H, Jeong T-S, Kim SU, Bae KS, Son K-H, Park H-Y (2009b) Novel GH10 xylanase, with a fibronectin type 3 domain, from Cellulosimicrobium sp. strain HY-13, a bacterium in the gut of Eisenia fetida. Appl Environ Microbiol 75:7275–7279
7. Kim DY, Han MK, Oh H-W, Park D-S, Kim S-J, Lee S-G, Shin D-H, Son K-H, Bae KS, Park H-Y (2010) Catalytic properties of a GH10 endo-β-1,4-xylanase from Streptomyces thermocarboxydus HY-15 isolated from the gut of Eisenia fetida. J Mol Catal B Enzym 62:32–39
8. Kim DY, Ham S-J, Lee HJ, Kim Y-J, Shin D-H, Rhee YH, Son K-H, Park H-Y (2011a) A highly active endo-β-1,4-mannanase produced by Cellulosimicrobium sp. strain HY-13, a hemicellulolytic bacterium in the gut of Eisenia fetida. Enzym Microb Technol 48:365–370
9. Kim DY, Ham S-J, Lee HJ, Cho H-Y, Kim J-H, Kim Y-J, Shin D-H, Rhee YH, Son K-H, Park H-Y (2011b) Cloning and characterization of a modular GH5 & #x03B2;-1,4-mannanase with high specific activity from the fibrolytic bacterium Cellulosimicrobium sp. strain HY-13. Bioresour Technol 102:9185–9192
10. Kim DY, Ham S-J, Kim HJ, Kim J, Lee M-H, Cho H-Y, Shin D-H, Rhee YH, Son K-H, Park H-Y (2012) Novel modular endo-β-1,4-xylanase with transglycosylation activity from Cellulosimicrobium sp. strain HY-13 that is homologous to inverting GH family 6 enzymes. Bioresour Technol 107:25–32
11. Kim DY, Shin D-H, Jung S, Lee JS, Cho H-Y, Bae KS, Sung C-K, Rhee YH, Son K-H, Park H-Y (2014a) Biocatalytic properties and substrate-binding ability of a modular GH10 & #x03B2;-1,4-xylanase from an insect-symbiotic bacterium, Streptomyces mexicanus HY-14. J Microbiol 52:863–870
12. Kim DY, Shin D-H, Jung S, Kim H, Lee JS, Cho H-Y, Bae KS, Sung C-K, Rhee YH, Son K-H, Park H-Y (2014b) Novel alkali-tolerant GH10 endo-β-1,4-xylanase with broad substrate specificity from Microbacterium trichothecenolyticum HY-17, a gut bacterium of the mole cricket Gryllotalpa orientalis. J Microbiol Biotechnol 24:943–953
13. Koivula A, Reinikainen T, Ruohonen L, Valkeajarvi A, Claeyssens M, Teleman O, Kleywegt GJ, Szardenings M, Rouvinen J, Jones TA, Teeri TT (1996) The active site of Trichoderma reesei cellobiohydrolase II: the role of tyrosine 169. Protein Eng 9:691–699
14. Koivula A, Ruohonen L, Wohlfahrt G, Reinikainen T, Teeri TT, Piens K, Claeyssens M, Weber M, Vasella A, Becker D, Sinnott ML, Zou JY, Kleywegt GJ, Szardenings M, Stahlberg J, Jones TA (2002) The active site of cellobiohydrolase Cel6A from Trichoderma reesei: the roles of aspartic acids D221 and D175. J Am Chem Soc 124:10015–10024
15. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428
16. Planas A (2000) Bacterial 1,3-1,4-β-glucanases: structure, function and protein engineering. Biochim Biophys Acta 1543:361–382
17. Poidevin L, Feliu J, Doan A, Berrin J-G, Bey M, Coutinho PM, Henrissat B, Record E, Heiss-Blanquet S (2013) Insights into exo- and endoglucanase activities of family 6 glycoside hydrolases from Podospora anserina. Appl Environ Microbiol 79:4220–4229
18. Rabinovich ML, Melnik MS, Bolovova AV (2002) Microbial cellulases. Appl Biochem Microbiol 38:305–321
19. Rouvinen J, Bergfors T, Teeri TT, Knowles JKC, Jones TA (1990) Three-dimensional structure of cellobiohydrolase II from Trichoderma reesei. Science 249:380–386
20. Schülein M (1997) Enzymatic properties of cellulases from Humicola insolens. J Biotechnol 57:71–81
21. Selinger LB, Forsberg CW, Cheng K-J (1996) The rumen: a unique source of enzymes for enhancing livestock production. Anaerobe 2:263–284
22. Sukumaran RK, Singhania RR, Pandey A (2005) Microbial cellulases-production, applications and challenges. J Sci Ind Res 64:832–844
23. Varrot A, Frandsen TP, von Ossowski I, Boyer V, Cottaz S, Driguez H, Schülein M, Davies GJ (2003) Structural basis for ligand binding and processivity in cellobiohydrolase Cel6A from Humicola insolens. Structure 11:855–864
24. Watanabe H, Tokuda G (2010) Cellulolytic systems in insects. Annu Rev Entomol 55:609–632
25. Zhao J, Shi P, Yuan T, Huang H, Li Z, Meng K, Yang P, Yao B (2012) Purification, gene cloning and characterization of an acidic & #x03B2;-1,4-glucanase from Phialophora sp. G5 with potential applications in the brewing and feed industries. J Biosci Bioeng 114:379–384