Purification of Protein Chaperones and Their Functional Assays with Intermediate Filaments

Methods in Enzymology

Volumn 569, Issue , 2016, Pages 155-175

  Perng, Ming Der ^a   Huang, Yu Shan ^a   Quinlan, Roy A ^b  

^a NATIONAL TSING HUA UNIVERSITY   (Taiwan)

^b DURHAM UNIVERSITY   (United Kingdom)

Author keywords

Cardiomyopathy; CRYAB; Desmin; GFAP; Proteinopathy; Proteostasis; Vimentin; B crystallin

Indexed keywords

CHAPERONE; INTERMEDIATE FILAMENT PROTEIN; SMALL HEAT SHOCK PROTEIN; CYTOSKELETON PROTEIN; HEAT SHOCK PROTEIN; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; CELL METABOLISM; CELLULAR STRESS RESPONSE; CONTROLLED STUDY; CRYSTAL STRUCTURE; CYTOSKELETON; ELECTRON MICROSCOPY; HOMEOSTASIS; IN VITRO STUDY; IN VIVO STUDY; INTERMEDIATE FILAMENT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN DEGRADATION; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SEDIMENTATION; STAINING; TISSUE METABOLISM; VISCOSITY; CHEMISTRY; ESCHERICHIA COLI; HUMAN; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; SIZE EXCLUSION CHROMATOGRAPHY;

CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; CYTOSKELETAL PROTEINS; ESCHERICHIA COLI; HEAT-SHOCK PROTEINS; HUMANS; INTERMEDIATE FILAMENTS; RECOMBINANT PROTEINS;

EID: 84954363461     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/bs.mie.2015.07.025     Document Type: Chapter

References (82)

1. J. Acunzo, M. Katsogiannou, and P. Rocchi Small heat shock proteins HSP27 (HspB1), alphaB-crystallin (HspB5) and HSP22 (HspB8) as regulators of cell death The International Journal of Biochemistry & Cell Biology 44 2012 1622 1631
2. C.M. Alam, J.S. Silvander, E.N. Daniel, G.Z. Tao, S.M. Kvarnstrom, P. Alam, and et al. Keratin 8 modulates beta-cell stress responses and normoglycaemia Journal of Cell Science 126 2013 5635 5644
3. J. Balogh, Z. Li, D. Paulin, and A. Arner Desmin filaments influence myofilament spacing and lateral compliance of slow skeletal muscle fibers Biophysical Journal 88 2005 1156 1165
4. E. Basha, H. O'Neill, and E. Vierling Small heat shock proteins and alpha-crystallins: Dynamic proteins with flexible functions Trends in Biochemical Sciences 37 2012 106 117
5. C. Bjorkdahl, M.J. Sjogren, X. Zhou, H. Concha, J. Avila, B. Winblad, and et al. Small heat shock proteins Hsp27 or alphaB-crystallin and the protein components of neurofibrillary tangles: Tau and neurofilaments Journal of Neuroscience Research 86 2008 1343 1352
6. W.C. Boelens, Y. Croes, and W.W. de Jong Interaction between alphaB-crystallin and the human 20S proteasomal subunit C8/alpha7 Biochimica et Biophysica Acta 1544 2001 311 319
7. A.M. Boriek, Y. Capetanaki, W. Hwang, T. Officer, M. Badshah, J. Rodarte, and et al. Desmin integrates the three-dimensional mechanical properties of muscles American Journal of Physiology. Cell Physiology 280 2001 C46 C52
8. A.C. Brewer, S.B. Mustafi, T.V. Murray, N.S. Rajasekaran, and I.J. Benjamin Reductive stress linked to small HSPs, G6PD, and Nrf2 pathways in heart disease Antioxidants & Redox Signaling 18 2013 1114 1127
9. Y. Capetanaki, S. Papathanasiou, A. Diokmetzidou, G. Vatsellas, and M. Tsikitis Desmin related disease: A matter of cell survival failure Current Opinion in Cell Biology 32 2015 113 120
10. L. Carlsson, Z.L. Li, D. Paulin, M.G. Price, J. Breckler, R.M. Robson, and et al. Differences in the distribution of synemin, paranemin, and plectin in skeletal muscles of wild-type and desmin knock-out mice Histochemistry and Cell Biology 114 2000 39 47
11. O. Chourbagi, F. Bruston, M. Carinci, Z. Xue, P. Vicart, D. Paulin, and et al. Desmin mutations in the terminal consensus motif prevent synemin-desmin heteropolymer filament assembly Experimental Cell Research 317 2011 886 897
12. D. Cox, J.A. Carver, and H. Ecroyd Preventing alpha-synuclein aggregation: The role of the small heat-shock molecular chaperone proteins Biochimica et Biophysica Acta 1842 2014 1830 1843
13. D.V. Dabir, J.Q. Trojanowski, C. Richter-Landsberg, V.M. Lee, and M.S. Forman Expression of the small heat-shock protein alphaB-crystallin in tauopathies with glial pathology The American Journal of Pathology 164 2004 155 166
14. J. den Engelsman, V. Keijsers, W.W. de Jong, and W.C. Boelens The small heat-shock protein alpha B-crystallin promotes FBX4-dependent ubiquitination The Journal of Biological Chemistry 278 2003 4699 4704
15. C. Eliasson, C. Sahlgren, C.H. Berthold, J. Stakeberg, J.E. Celis, C. Betsholtz, and et al. Intermediate filament protein partnership in astrocytes The Journal of Biological Chemistry 274 1999 23996 24006
16. J.L. Elliott, M. Der Perng, A.R. Prescott, K.A. Jansen, G.H. Koenderink, and R.A. Quinlan The specificity of the interaction between alphaB-crystallin and desmin filaments and its impact on filament aggregation and cell viability Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences 368 2013 20120375
17. B. Favre, Y. Schneider, P. Lingasamy, J.E. Bouameur, N. Begre, Y. Gontier, and et al. Plectin interacts with the rod domain of type III intermediate filament proteins desmin and vimentin European Journal of Cell Biology 90 2011 390 400
18. K.P. Garcia-Pelagio, J. Muriel, A. O'Neill, P.F. Desmond, R.M. Lovering, L. Lund, and et al. Myopathic changes in murine skeletal muscle lacking synemin American Journal of Physiology. Cell Physiology 308 2015 C448 C462
19. L.G. Goldfarb, K.Y. Park, L. Cervenakova, S. Gorokhova, H.S. Lee, O. Vasconcelos, and et al. Missense mutations in desmin associated with familial cardiac and skeletal myopathy Nature Genetics 19 1998 402 403
20. R.D. Goldman, B. Grin, M.G. Mendez, and E.R. Kuczmarski Intermediate filaments: Versatile building blocks of cell structure Current Opinion in Cell Biology 20 2008 28 34
21. K.J. Green, M. Bohringer, T. Gocken, and J.C. Jones Intermediate filament associated proteins Advances in Protein Chemistry 70 2005 143 202
22. J.R. Harris Negative staining of thinly spread biological particulates Methods in Molecular Biology 117 1999 13 30
23. H. Herrmann, and U. Aebi Intermediate filaments and their associates: Multi-talented structural elements specifying cytoarchitecture and cytodynamics Current Opinion in Cell Biology 12 2000 79 90
24. H. Herrmann, and U. Aebi Intermediate filaments: Molecular structure, assembly mechanism, and integration into functionally distinct intracellular scaffolds Annual Review of Biochemistry 73 2004 749 789
25. H. Herrmann, H. Bar, L. Kreplak, S.V. Strelkov, and U. Aebi Intermediate filaments: From cell architecture to nanomechanics Nature Reviews. Molecular Cell Biology 8 2007 562 573
26. H. Herrmann, S.V. Strelkov, P. Burkhard, and U. Aebi Intermediate filaments: Primary determinants of cell architecture and plasticity The Journal of Clinical Investigation 119 2009 1772 1783
27. M. Hesse, T.M. Magin, and K. Weber Genes for intermediate filament proteins and the draft sequence of the human genome: Novel keratin genes and a surprisingly high number of pseudogenes related to keratin genes 8 and 18 Journal of Cell Science 114 2001 2569 2575
28. K. Hnia, H. Tronchere, K.K. Tomczak, L. Amoasii, P. Schultz, A.H. Beggs, and et al. Myotubularin controls desmin intermediate filament architecture and mitochondrial dynamics in human and mouse skeletal muscle The Journal of Clinical Investigation 121 2011 70 85
29. I. Hofmann Measuring the assembly kinetics and binding properties of intermediate filament proteins Sub-Cellular Biochemistry 31 1998 363 380
30. W.F. Hu, L. Gong, Z. Cao, H. Ma, W. Ji, M. Deng, and et al. AlphaA- and alphaB-crystallins interact with caspase-3 and Bax to guard mouse lens development Current Molecular Medicine 12 2012 177 187
31. T.W. Huiatt, R.M. Robson, N. Arakawa, and M.H. Stromer Desmin from avian smooth muscle. Purification and partial characterization The Journal of Biological Chemistry 255 1980 6981 6989
32. R. Joubert, A. Vignaud, M. Le, C. Moal, N. Messaddeq, and A. Buj-Bello Site-specific Mtm1 mutagenesis by an AAV-Cre vector reveals that myotubularin is essential in adult muscle Human Molecular Genetics 22 2013 1856 1866
33. G. Kappe, E. Franck, P. Verschuure, W.C. Boelens, J.A. Leunissen, and W.W. de Jong The human genome encodes 10 alpha-crystallin-related small heat shock proteins: HspB1-10 Cell Stress & Chaperones 8 2003 53 61
34. M.W. Kemp, B. Edwards, M. Burgess, W.T. Clarke, G. Nicholson, D.A. Parry, and et al. Syncoilin isoform organization and differential expression in murine striated muscle Journal of Structural Biology 165 2009 196 203
35. S. Kim, and P.A. Coulombe Intermediate filament scaffolds fulfill mechanical, organizational, and signaling functions in the cytoplasm Genes & Development 21 2007 1581 1597
36. S. Kim, P. Wong, and P.A. Coulombe A keratin cytoskeletal protein regulates protein synthesis and epithelial cell growth Nature 441 2006 362 365
37. A. Landsbury, M.D. Perng, E. Pohl, and R.A. Quinlan Functional symbiosis between the intermediate filament cytoskeleton and small heat shock proteins S. Simon, A.P. Arigo, Small stress proteins and human diseases 2010 Nova Science Hauppauge, New York 55 87
38. E. Lazarides Intermediate filaments as mechanical integrators of cellular space Nature 283 1980 249 256
39. C. Leduc, and S. Etienne-Manneville Intermediate filaments in cell migration and invasion: The unusual suspects Current Opinion in Cell Biology 32C 2015 102 112
40. M. Li, M. Andersson-Lendahl, T. Sejersen, and A. Arner Knockdown of desmin in zebrafish larvae affects interfilament spacing and mechanical properties of skeletal muscle The Journal of General Physiology 141 2013 335 345
41. H. Li, Y. Guo, J. Teng, M. Ding, A.C. Yu, and J. Chen 14-3-3gamma affects dynamics and integrity of glial filaments by binding to phosphorylated GFAP Journal of Cell Science 119 2006 4452 4461
42. Z. Li, A. Parlakian, D. Coletti, S. Alonso-Martin, C. Hourde, P. Joanne, and et al. Synemin acts as a regulator of signalling molecules during skeletal muscle hypertrophy Journal of Cell Science 127 2014 4589 4601
43. J.P. Liu, R. Schlosser, W.Y. Ma, Z. Dong, H. Feng, L. Liu, and et al. Human alphaA- and alphaB-crystallins prevent UVA-induced apoptosis through regulation of PKCalpha, RAF/MEK/ERK and AKT signaling pathways Experimental Eye Research 79 2004 393 403
44. L.M. Lund, J.P. Kerr, J. Lupinetti, Y. Zhang, M.A. Russell, R.J. Bloch, and et al. Synemin isoforms differentially organize cell junctions and desmin filaments in neonatal cardiomyocytes The FASEB Journal 26 2012 137 148
45. S. MacLean-Fletcher, and T.D. Pollard Identification of a factor in conventional muscle actin preparations which inhibits actin filament self-association Biochemical and Biophysical Research Communications 96 1980 18 27
46. K.J. McCullagh, B. Edwards, M.W. Kemp, L.C. Giles, M. Burgess, and K.E. Davies Analysis of skeletal muscle function in the C57BL6/SV129 syncoilin knockout mouse Mammalian Genome: Official Journal of the International Mammalian Genome Society 19 2008 339 351
47. D.J. Milner, G. Weitzer, D. Tran, A. Bradley, and Y. Capetanaki Disruption of muscle architecture and myocardial degeneration in mice lacking desmin The Journal of Cell Biology 134 1996 1255 1270
48. G. Morrow, and R.M. Tanguay Small heat shock protein expression and functions during development The International Journal of Biochemistry & Cell Biology 44 2012 1613 1621
49. A.M. Munoz-Marmol, G. Strasser, M. Isamat, P.A. Coulombe, Y. Yang, X. Roca, and et al. A dysfunctional desmin mutation in a patient with severe generalized myopathy Proceedings of the National Academy of Sciences of the United States of America 95 1998 11312 11317
50. I.D. Nicholl, and R.A. Quinlan Chaperone activity of alpha-crystallins modulates intermediate filament assembly The EMBO Journal 13 1994 945 953
51. M. Nieminen, T. Henttinen, M. Merinen, F. Marttila-Ichihara, J.E. Eriksson, and S. Jalkanen Vimentin function in lymphocyte adhesion and transcellular migration Nature Cell Biology 8 2006 156 162
52. H.M. Pallari, and J.E. Eriksson Intermediate filaments as signaling platforms Science's STKE 2006 2006 53
53. D.A. Parry Microdissection of the sequence and structure of intermediate filament chains Advances in Protein Chemistry 70 2005 113 142
54. M.D. Perng, L. Cairns, I.P. van den, A. Prescott, A.M. Hutcheson, and R.A. Quinlan Intermediate filament interactions can be altered by HSP27 and alphaB-crystallin Journal of Cell Science 112 Pt. 13 1999 2099 2112
55. M.D. Perng, and R.A. Quinlan The dynamic duo of small heat proteins and Ifs maintain cell homeostasis, resist cellular stress and enable evolution in cells and tissues R.M. Tanguay, L.E. Hightower, The big book of small heat shock proteins 2015 Springer International Publishing AG 401 434
56. M.D. Perng, A. Sandilands, J. Kuszak, R. Dahm, A. Wegener, A.R. Prescott, and et al. The intermediate filament systems in the eye lens Methods in Cell Biology 78 2004 597 624
57. M.D. Perng, S.F. Wen, T. Gibbon, J. Middeldorp, J. Sluijs, E.M. Hol, and et al. Glial fibrillary acidic protein filaments can tolerate the incorporation of assembly-compromised GFAP-delta, but with consequences for filament organization and alphaB-crystallin association Molecular Biology of the Cell 19 2008 4521 4533
58. M.D. Perng, S.F. Wen, I.P. van den, A.R. Prescott, and R.A. Quinlan Desmin aggregate formation by R120G alphaB-crystallin is caused by altered filament interactions and is dependent upon network status in cells Molecular Biology of the Cell 15 2004 2335 2346
59. E. Poon, E.V. Howman, S.E. Newey, and K.E. Davies Association of syncoilin and desmin: Linking intermediate filament proteins to the dystrophin-associated protein complex The Journal of Biological Chemistry 277 2002 3433 3439
60. R. Quinlan Cytoskeletal competence requires protein chaperones Progress in Molecular and Subcellular Biology 28 2002 219 233
61. R.A. Quinlan, and R.J. Ellis Chaperones: Needed for both the good times and the bad times Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences 368 2013 20130091
62. R. Quinlan, and P. Van Den Ijssel Fatal attraction: When chaperone turns harlot Nature Medicine 5 1999 25 26
63. R.F. Roelofs, D.F. Fischer, S.H. Houtman, J.A. Sluijs, W. Van Haren, F.W. Van Leeuwen, and et al. Adult human subventricular, subgranular, and subpial zones contain astrocytes with a specialized intermediate filament cytoskeleton Glia 52 2005 289 300
64. J. Satoh, T. Yamamura, and K. Arima The 14-3-3 protein epsilon isoform expressed in reactive astrocytes in demyelinating lesions of multiple sclerosis binds to vimentin and glial fibrillary acidic protein in cultured human astrocytes The American Journal of Pathology 165 2004 577 592
65. R. Schroder, I. Warlo, H. Herrmann, P.F. van der Ven, C. Klasen, I. Blumcke, and et al. Immunogold EM reveals a close association of plectin and the desmin cytoskeleton in human skeletal muscle European Journal of Cell Biology 78 1999 288 295
66. S.C. Schweitzer, M.W. Klymkowsky, R.M. Bellin, R.M. Robson, Y. Capetanaki, and R.M. Evans Paranemin and the organization of desmin filament networks Journal of Cell Science 114 2001 1079 1089
67. D. Selcen Myofibrillar myopathies Neuromuscular Disorders 21 2011 161 171
68. K. Seltmann, A.W. Fritsch, J.A. Kas, and T.M. Magin Keratins significantly contribute to cell stiffness and impact invasive behavior Proceedings of the National Academy of Sciences of the United States of America 110 2013 18507 18512
69. S.B. Shah, J.M. Love, A. O'Neill, R.M. Lovering, and R.J. Bloch Influences of desmin and keratin 19 on passive biomechanical properties of mouse skeletal muscle Journal of Biomedicine & Biotechnology 2012 2012 704061
70. R.K. Sihag, M. Inagaki, T. Yamaguchi, T.B. Shea, and H.C. Pant Role of phosphorylation on the structural dynamics and function of types III and IV intermediate filaments Experimental Cell Research 313 2007 2098 2109
71. N.T. Snider, and M.B. Omary Post-translational modifications of intermediate filament proteins: Mechanisms and functions Nature Reviews. Molecular Cell Biology 15 2014 163 177
72. S. Tabor, and C.C. Richardson A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes Proceedings of the National Academy of Sciences of the United States of America 82 1985 1074 1078
73. D.M. Toivola, G.Z. Tao, A. Habtezion, J. Liao, and M.B. Omary Cellular integrity plus: Organelle-related and protein-targeting functions of intermediate filaments Trends in Cell Biology 15 2005 608 617
74. T.M. Treweek, S. Meehan, H. Ecroyd, and J.A. Carver Small heat-shock proteins: Important players in regulating cellular proteostasis Cellular and Molecular Life Sciences 72 2015 429 451
75. E. Tsoupri, and Y. Capetanaki Myospryn: A multifunctional desmin-associated protein Histochemistry and Cell Biology 140 2013 55 63
76. P. van Oosten-Hawle, and R.I. Morimoto Organismal proteostasis: Role of cell-nonautonomous regulation and transcellular chaperone signaling Genes & Development 28 2014 1533 1543
77. P. Vicart, A. Caron, P. Guicheney, Z. Li, M.C. Prevost, A. Faure, and et al. A missense mutation in the alphaB-crystallin chaperone gene causes a desmin-related myopathy Nature Genetics 20 1998 92 95
78. G. Wettstein, P.S. Bellaye, O. Micheau, and P. Bonniaud Small heat shock proteins and the cytoskeleton: An essential interplay for cell integrity? The International Journal of Biochemistry & Cell Biology 44 2012 1680 1686
79. L. Winter, and G. Wiche The many faces of plectin and plectinopathies: Pathology and mechanisms Acta Neuropathologica 125 2013 77 93
80. T. Wisniewski, and J.E. Goldman Alpha B-crystallin is associated with intermediate filaments in astrocytoma cells Neurochemical Research 23 1998 385 392
81. Z.G. Xue, Y. Cheraud, V. Brocheriou, A. Izmiryan, M. Titeux, D. Paulin, and et al. The mouse synemin gene encodes three intermediate filament proteins generated by alternative exon usage and different open reading frames Experimental Cell Research 298 2004 431 444
82. J. Zhang, M.L. Bang, D.S. Gokhin, Y. Lu, L. Cui, X. Li, and et al. Syncoilin is required for generating maximum isometric stress in skeletal muscle but dispensable for muscle cytoarchitecture American Journal of Physiology. Cell Physiology 294 2008 C1175 C1182