Rapid and Specific Diagnosis of Extrapulmonary Tuberculosis by Immunostaining of Tissues and Aspirates with Anti-MPT64

Applied Immunohistochemistry and Molecular Morphology

Volumn 25, Issue 4, 2017, Pages 282-288

  Purohit, Manju R ^a,b,c   Sviland, Lisbet ^d   Wiker, Harald ^e   Mustafa, Tehmina ^d,e  

^a RD Gardi Medical College   (India)

^b RD Gardi Medical College   (India)

^c KAROLINSKA INSTITUTE   (Sweden)

^d HAUKELAND UNIVERSITY HOSPITAL   (Norway)

^e UNIVERSITY OF BERGEN   (Norway)

Author keywords

anti MPT64; diagnosis; immunostaining; tuberculosis

Indexed keywords

ARTICLE; CELL DEATH; CELL STRUCTURE; CONTROLLED STUDY; CYTOLOGY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; EXTRAPULMONARY TUBERCULOSIS; FINE NEEDLE ASPIRATION BIOPSY; GRANULOMA; HUMAN; HUMAN TISSUE; IMMUNOCYTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; LUNG ASPIRATION; MAJOR CLINICAL STUDY; MYCOBACTERIUM TUBERCULOSIS; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; IMMUNOLOGY; LYMPH NODE; MICROBIOLOGY; PATHOLOGY; PHYSIOLOGY; PLEURA; TUBERCULOSIS;

BACTERIAL ANTIGEN; MPT64 PROTEIN, MYCOBACTERIUM TUBERCULOSIS;

ANTIGENS, BACTERIAL; BIOPSY, FINE-NEEDLE; HUMANS; IMMUNOHISTOCHEMISTRY; LYMPH NODES; MYCOBACTERIUM TUBERCULOSIS; PLEURA; PREDICTIVE VALUE OF TESTS; SENSITIVITY AND SPECIFICITY; TUBERCULOSIS;

EID: 84954350384     PISSN: 15412016     EISSN: 15334058     Source Type: Journal    
DOI: 10.1097/PAI.0000000000000300     Document Type: Article

References (23)

1. Rothschild BM, Martin LD, Lev G, et al. Mycobacterium tuberculosis complex DNA from an extinct bison dated 17,000 years before the present. Clin Infect Dis. 2001;33:305-311.
2. Kanlikama M, Mumbuc S, Bayazit Y, et al. Management strategy of mycobacterial cervical lymphadenitis. J Laryngol Otol. 2000;114: 274-278.
3. World Health Organization. Global Tuberculosis Report. Geneva, Switzerland: World Health Organization; 2012.
4. Norwegian Institue of Public Health. Tuberkuloseveilederen [Tuberculosis guide]. 2010.
5. Global tuberculosis control, WHO report 2001, WHO/CDS/TB/ 2001.287. Communicable Diseases, World Health Organisation, Geneva 2001: p8-34.
6. World Health Organization. Global tuberculosis control: WHO report. 2013. Available at: http://www.who.int/tb/publications/ global-report/en/index.html. 2014.
7. Gutierrez C, Garcia M, J F. Comparison of Ziehl-Neelsen staining and immunohistochemistry for detection of Mycobacterium bovis in bovine and caprine tuberculosis lesions. J Comp Pathol. 1993;109: 361-370.
8. Manju Raj Purohit TM. Laboratory diagnosis of extra-pulmonary tuberculosis (EPTB) in resource-constrained setting: state of the art, challenges and the need. J Clin Diagn Res. 2015;9:EE01-EE06.
9. DiBonito L, Falconieri G, Colautti I, et al. The positive pleural effusion. A retrospective study of cytopathologic diagnoses with autopsy confirmation. Acta Cytol. 1992;36:329-332.
10. James P, Gupta R, Christopher DJ, et al. Evaluation of the diagnostic yield and safety of closed pleural biopsy in the diagnosis of pleural effusion. Indian J Tuberc. 2010;57:19-24.
11. Kirsch CM, Kroe DM, Azzi RL, et al. The optimal number of pleural biopsy specimens for a diagnosis of tuberculous pleurisy. Chest. 1997;112:702-706.
12. Purohit MR, Mustafa T, Wiker HG, et al. Immunohistochemical diagnosis of abdominal and lymph node tuberculosis by detecting Mycobacterium tuberculosis complex specific antigen MPT64. Diagn Pathol. 2007;2:36.
13. Purohit MR, Mustafa T, Wiker HG, et al. Rapid diagnosis of tuberculosis in aspirate, effusions, and cerebrospinal fluid by immunocytochemical detection of Mycobacterium tuberculosis complex specific antigen MPT64. Diagn Cytopathol. 2012;40:782-791.
14. Baba K, Dyrhol-Riise AM, Sviland L, et al. Rapid and specific diagnosis of tuberculous pleuritis with immunohistochemistry by detecting Mycobacterium tuberculosis complex specific antigen MPT64 in patients from a HIV endemic area. Appl Immunohistochem Mol Morphol. 2008;16:554-561.
15. MustafaT,WikerHG,Mfinanga SG, et al. Immunohistochemistry using a Mycobacterium tuberculosis complex specific antibody for improved diagnosis of tuberculous lymphadenitis. Mod Pathol. 2006;19:1606-1614.
16. Purohit MR, Mustafa T, Sviland L. Detection of Mycobacterium tuberculosis by polymerase chain reaction with DNA eluted from aspirate smears of tuberculous lymphadenitis. Diagn Mol Pathol. 2008;17:174-178.
17. Friedrich SO, von Groote-Bidlingmaier F, Diacon AH. Xpert MTB/ RIF assay for diagnosis of pleural tuberculosis. J Clin Microbiol. 2011;49:4341-4342.
18. Tortoli E, Russo C, Piersimoni C, et al. Clinical validation of Xpert MTB/RIF for the diagnosis of extrapulmonary tuberculosis. Eur Respir J. 2012;40:442-447.
19. Vadwai V, Boehme C, Nabeta P, et al. Xpert MTB/RIF: a new pillar in diagnosis of extrapulmonary tuberculosis? J Clin Microbiol. 2011; 49:2540-2545.
20. Zeka AN, Tasbakan S, Cavusoglu C. Evaluation of the GeneXpert MTB/RIF assay for rapid diagnosis of tuberculosis and detection of rifampin resistance in pulmonary and extrapulmonary specimens. J Clin Microbiol. 2011;49:4138-4141.
21. Arrese JE, Pierard GE. Immunostaining of various micro-organisms by a polyclonal anti-Mycobacterium bovis antibody. J Am Acad Dermatol. 1998;39(pt 1):810.
22. Kutzner H, Argenyi ZB, Requena L, et al. A new application of BCG antibody for rapid screening of various tissue microorganisms. J Am Acad Dermatol. 1998;38:56-60.
23. Closs O, Harboe M, Axelsen NH, et al. The antigens of Mycobacterium bovis, strain BCG, studied by crossed immunoelectrophoresis: a reference system. Scand J Immunol. 1980;12:249-263.