Expression of Shewanella frigidimarina fatty acid metabolic genes in E. coli by CRISPR/cas9-coupled lambda Red recombineering

Biotechnology Letters

Volumn 38, Issue 1, 2016, Pages 117-122

  Xia, Jun ^a   Wang, Ling ^b   Zhu, Jian bo ^a   Sun, Cheng jun ^c   Zheng, Ming gang ^c   Zheng, Li ^c   Lou, Ying hua ^b   Shi, Lei ^b  

^a SHIHEZI UNIVERSITY   (China)

^b QINGDAO UNIVERSITY   (China)

^c FIRST INSTITUTE OF OCEANOGRAPHY   (China)

Author keywords

Acetyl CoA carboxylase; CRISPR cas9; Fatty acid regulatory transcription factor; Lipid production; Shewanella frigidimarina; 9 Desaturase; Red recombinases

Indexed keywords

BACTERIA; BIODEGRADATION; CLONING; ESCHERICHIA COLI; GENE EXPRESSION; GENES; MACHINERY; METABOLISM; TRANSCRIPTION; TRANSCRIPTION FACTORS;

ACETYL-COA CARBOXYLASE; CRISPR/CAS9; DESATURASE; LIPID PRODUCTIONS; RECOMBINASES; SHEWANELLA;

FATTY ACIDS;

BACTERIAL PROTEIN; FATTY ACID; RECOMBINANT PROTEIN; RECOMBINASE;

CRISPR CAS SYSTEM; ENTEROBACTERIA PHAGE LAMBDA; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; SHEWANELLA;

BACTERIAL PROTEINS; BACTERIOPHAGE LAMBDA; CLONING, MOLECULAR; CRISPR-CAS SYSTEMS; ESCHERICHIA COLI; FATTY ACIDS; GENE EXPRESSION; RECOMBINANT PROTEINS; RECOMBINASES; SHEWANELLA;

EID: 84953635026     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-015-1956-4     Document Type: Article

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