Measuring the frequency of latent HIV-1 in resting CD4+ T cells using a limiting dilution coculture assay

Methods in Molecular Biology

Volumn 1354, Issue , 2016, Pages 239-253

  Laird, Gregory M ^a   Rosenbloom, Daniel I S ^a   Lai, Jun ^a   Siliciano, Robert F ^a   Siliciano, Janet D ^a  

^a NONE

Author keywords

HIV 1; Latency; Latent reservoir; Viral outgrowth assay

Indexed keywords

COMPLEMENTARY DNA; INTERLEUKIN 2; REAGENT; ANTI HUMAN IMMUNODEFICIENCY VIRUS AGENT;

CD4+ T LYMPHOCYTE; CELL ASSAY; CELL ISOLATION; COCULTURE; CONTROLLED STUDY; CULTURE MEDIUM; DILUTION; DNA SYNTHESIS; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 1 INFECTION; LABORATORY DEVICE; LATENT VIRUS INFECTION; LIMITING DILUTION COCULTURE ASSAY; LYMPHOBLAST; NONHUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; QUANTITATIVE VIRUS OUTGROWTH ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; TARGET CELL; VIRUS CHARACTERIZATION; VIRUS LATENCY; VIRUS REPLICATION; CELL CULTURE; DRUG EFFECTS; HIV INFECTIONS; PHYSIOLOGY; PROCEDURES; VIROLOGY; VIRUS CULTURE;

ANTI-HIV AGENTS; CD4-POSITIVE T-LYMPHOCYTES; CELLS, CULTURED; COCULTURE TECHNIQUES; HIV INFECTIONS; HIV-1; HUMANS; VIRUS CULTIVATION; VIRUS LATENCY;

EID: 84952913716     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-3046-3_16     Document Type: Chapter

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