Molecular assay optimized by Taguchi experimental design method for venous thromboembolism investigation

Molecular and Cellular Probes

Volumn 25, Issue 5-6, 2011, Pages 231-237

  Celani de Souza, Helder Jose ^a,b   Moyses, Cinthia B ^b   Pontes, Fabrício J ^a   Duarte, Roberto N ^c   Sanches da Silva, Carlos Eduardo ^d   Alberto, Fernando Lopes ^b   Ferreira, Ubirajara R ^a   Silva, Messias Borges ^a,e  

^a SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

^b Department of Innovation Research and Development ^*  (Brazil)

^c Ciência e Tecnologia de Roraima   (Brazil)

^d FEDERAL UNIVERSITY OF ITAJUBÁ   (Brazil)

^e UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Design of experiments; FRET; Genotyping; Real time PCR; Taguchi

Indexed keywords

DNA;

ANALYTIC METHOD; ARTICLE; ASSAY; CLINICAL EFFECTIVENESS; DIAGNOSTIC PROCEDURE; EXPERIMENTAL DESIGN; FLUORESCENCE RESONANCE ENERGY TRANSFER; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; TAGUCHI EXPERIMENTAL DESIGN METHOD; VENOUS THROMBOEMBOLISM;

ALGORITHMS; DNA MUTATIONAL ANALYSIS; FACTOR ANALYSIS, STATISTICAL; FACTOR V; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENOTYPE; HUMANS; MUTATION; PROTHROMBIN; REAL-TIME POLYMERASE CHAIN REACTION; VENOUS THROMBOEMBOLISM;

EID: 80055075091     PISSN: 08908508     EISSN: 10961194     Source Type: Journal    
DOI: 10.1016/j.mcp.2011.08.001     Document Type: Article

References (18)

1. Guidelines on investigation and management of thrombophilia. J Clin Pathol 1990, 43:703-710. British Committee for Standards in Haematology.
2. Rosendaal F.R. Venous thrombosis: a multicausal disease. Lancet 1999, 353:1167-1173.
3. Franco R.F., Reitsma P.H. Genetic risk factors of venous thrombosis. Hum Genet 2001, 109:369-384.
4. Lay M.J., Wittwer C.T. Real-time fluorescence genotyping of factor V Leiden during rapid-cycle PCR. Clin Chem 1997, 43:2262-2267.
5. von Ahsen N., Schütz E., Armstrong V.W., Oellerich M. Rapid detection of prothrombotic mutations of Prothrombin (G20210A), Factor V Leiden (G1691A), and methylenetetrahydrofolate reductase (C677T) by real-time fluorescence PCR with the LightCycler. Clin Chem 1999, 45:694-696.
6. Neoh S.H., Brisco M.J., Firgaira F.A., Trainor K.J., Turner D.R., Morley A.A. Rapid detection of the factor V Leiden (1691 G > A) and haemochromatosis (845 G > A) mutation by fluorescence resonance energy transfer (FRET) and real time PCR. J Clin Pathol 1999, 52:766-769.
7. Sevall J.S., Factor V. Leiden genotyping using real-time fluorescent polymerase chain reaction. Mol Cell Probes 2000, 14:249-253.
8. Parks S.B., Popovich B.W., Press R.D. Real-time polymerase chain reaction with fluorescent hybridization probes for the detection of prevalent mutations causing common thrombophilic and iron overload phenotypes. Am J Clin Pathol 2001, 115:439-447.
9. Ameziane N., Lamotte M., Lamoril J., Lebret D., Deybach J.C., Kaiser T., et al. Combined Factor V Leiden (G1691A) and Prothrombin (G20210A) genotyping by multiplex real-time polymerase chain reaction using fluorescent resonance energy transfer hybridization probes on the Rotor-Gene 2000. Blood Coagul Fibrinolysis 2003, 14:421-424.
10. Padke M.S. Quality engineering using robust design 1989, Prentice Hall, Englewood Cliffs, NJ.
11. Ross P.J. Taguchi techniques for quality engineering 1996, McGraw-Hill, USA, pp. 43-73.
12. Barrado E., Vega M., Grande P., Del Valle J.L. Optimization of a purification method for metal-containing wastewater by use of a Taguchi experimental design. Water Res 1996, 30(10):2309-2314.
13. Taguchi G., Konishi S. Taguchi methods: orthogonal arrays and linear graphs 1987, American Supplier Institute, USA.
14. Ballantyne K.N., van Oorschot R.A., Mitchell R.J. Reduce optimisation time and effort: Taguchi experimental design methods. Forensic Sci Int: Genet 2008, (Suppl. 1):7-8.
15. Montgomery D.C. Design and analysis of experiments 2003, John Wiley & Sons, NY.
16. Taguchi G., Elsayed E.A., Hsiang T. Quality engineering in the production systems 1987, McGraw-Hill.
17. Montgomery D.C., Runger G.C. Applied statistics and probability for engineers 2007, John Wiley & Sons, New York.
18. Vining G.G., Myers R.H. Combining Taguchi and response surface philosophies: a dual response approach. J Qual Technol 1990, 22:38-45.