A novel multiplex isothermal amplification method for rapid detection and identification of viruses

Scientific Reports

Volumn 5, Issue , 2015, Pages

  Nyan, Dougbeh Chris ^a   Swinson, Kevin L ^b  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^b MORGAN STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS DNA; VIRUS RNA;

CLASSIFICATION; GENETICS; HUMAN; NUCLEIC ACID AMPLIFICATION; PROCEDURES; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SPECTROFLUOROMETRY; STANDARDS; VIROLOGY; VIRUS; VIRUS DISEASES;

DNA, VIRAL; HUMANS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; REPRODUCIBILITY OF RESULTS; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, FLUORESCENCE; VIRUS DISEASES; VIRUSES;

EID: 84949519819     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep17925     Document Type: Article

References (23)

1. Centers for Disease Control and Prevention. Surveillance for Acute Viral Hepatitis in the United States. 2007. Surveillance Summaries, May. 22, 2009. MMWR 58, No. SS-3 (2009).
2. Drobeniuc J., et al. Laboratory-based surveillance for hepatitis E virus infection, United States, 2005-2012. Emerg Infect Dis. 19, 218-222 (2013).
3. Huy B. V., Vernavong K., & Kính N. V. HBV and HCV Coinfection among HIV/AIDS Patients in the National Hospital of Tropical Diseases, Vietnam. AIDS Res Treat 2014 581021. doi: 10.1155/2014/581021 (2014).
4. Naze F., et al. Simultaneous detection and quantitation of Chikungunya, dengue and West Nile viruses by multiplex RT-PCR assays and dengue virus typing using high resolution melting. J Virol Methods. 162, 1-7 (2009).
5. Zhou Y. H., et al. Comparison of HIV-, HBV-, HCV- And Co-Infection Prevalence between Chinese and Burmese Intravenous Drug Users of the China-Myanmar Border Region. PLoS One 6, e16349 (2011).
6. Maartens G., Celum C., & Lewin S. R. HIV infection: epidemiology, pathogenesis, treatment, and prevention. Lancet. 384, 258-271 (2014).
7. Dauphin, Zientara S, Zeller H., & Murgue B. West Nile: worldwide current situation in animals and humans. Comp Immunol Microbiol Infect Dis. 27, 343-355 (2004).
8. Dong C., et al. Restricted enzooticity of hepatitis E virus genotypes 1 to 4 in the United States. J Clin Microbiol. 49, 4164-4172 (2011).
9. Guzman M. G., et al. Dengue: a continuing global threat. Nat Rev Microbiol. 8, S7-16 (2010).
10. Beck C., et al. Flaviviruses in Europe: Complex Circulation Patterns and Their Consequences for the Diagnosis and Control of West Nile Disease. Int J Environ Res Public Health. 11, 6049-6083 (2013).
11. Chao D. Y., Davis B. S., & Chang G. J. Development of multiplex real-Time reverse transcriptase PCR assays for detecting eight medically important flaviviruses in mosquitoes. J Clin Microbiol. 45, 584-589 (2007).
12. Hennechart-Collette C., Martin-Latil S., Guillier L., & Perelle S. Multiplex real-Time RT-qPCR for the detection of Norovirus in bottled and tap water using murine norovirus as a process control. J Appl Microbiol. 116, 179-190 (2014
13. Notomi T., et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28, e63 (2000).
14. Fukuma A., et al. Rapid detection of Lassa virus by reverse transcription-loop mediated isothermal amplification, Microbiol Immunol. 55, 44-50 (2011).
15. Blomström A. L., et al A one-step reverse transcriptase loop-mediated isothermal amplification assay for simple and rapid detection of swine vesicular disease virus. J Virol Methods. 147, 188-193 (2008).
16. Wang et al A one-step reverse transcription loop-mediated isothermal amplification for detection and discrimination of infectious bursal disease virus. Virology Journal. 8, 108 (2011).
17. Nyan D. C., et al. Rapid Detection of Hepatitis B Virus in Blood Plasma by a Specific and Sensitive Loop-Mediated Isothermal Amplification Assay. Clin Infect Dis. 59, 16-23 (2014).
18. Jung J. H., et al. Combination of multiplex reverse-Transcription loop-mediated isothermal amplification with an immunochromatographic strip for subtyping influenza A virus. Analytica Chimica Acta. 853, 541-547 (2015).
19. Walker G. T., et al. Strand displacement amplification-An isothermal, in vitro DNA amplification technique. Nucleic Acids Res. 20, 1691-1696 (1992).
20. Tomita, N, Mori Y., Kanda H., & Notomi T. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat. Prot. 3, 877-882 (2008).
21. Beauchamp S., D'Auria S., Pennacchio A., & Lacroix M A new competitive fluorescence immunoassay for detection of Listeria monocytogenes. Anal. Methods. 4, 4187-4192 (2012).
22. Marras S. A. Interactive fluorophore and quencher pairs for labeling fluorescent nucleic acid hybridization probes. Mol Biotechnology. 38, 247-255 (2008).
23. Ginocchio C. C. Life Beyond PCR: Alternative Target Amplification Technologies for the Diagnosis of Infectious Diseases, Part II Clinical Microbiology Newsletter. 26, 129-136 (2004).