Evaluation of the broad-range PCR/ESI-MS technology in blood specimens for the molecular diagnosis of bloodstream Infections

PLoS ONE

Volumn 10, Issue 10, 2015, Pages

  Jordana Lluch, Elena ^a,b,c   Giménez, Montserrat ^a,b   Dolores Quesada, M ^a   Rivaya, Belén ^a   Marcó, Clara ^a   Jesus Dominguez M ^a   Arméstar, Fernando ^a   Martró, Elisa ^a,c,d   Ausina, Vicente ^a,b,c  

^a HOSPITAL UNIVERSITARI GERMANS TRIAS I PUJOL   (Spain)

^b INSTITUTO DE SALUD CARLOS III   (Spain)

^c Germans Trias i Pujol   (Spain)

^d CIBER EPIDEMIOLOGÍA Y SALUD PÚBLICA CIBERESP   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; AGED; ANAEROBIC BACTERIUM; ANTIBIOTIC THERAPY; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BLOOD CULTURE; BLOOD SAMPLING; BLOODSTREAM INFECTION; CONTROLLED STUDY; DIAGNOSTIC KIT; DIAGNOSTIC TEST ACCURACY STUDY; ELECTROSPRAY MASS SPECTROMETRY; EMERGENCY WARD; FEMALE; FUNGAL DETECTION; FUNGUS; FUNGUS CULTURE; GENE AMPLIFICATION; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; HOSPITAL ADMISSION; HUMAN; HUMAN CELL; INTENSIVE CARE UNIT; MAJOR CLINICAL STUDY; MALE; MOLECULAR DIAGNOSIS; NONHUMAN; OBSERVATIONAL STUDY; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE; PROSPECTIVE STUDY; SENSITIVITY AND SPECIFICITY; VERY ELDERLY; ADOLESCENT; ANTIBIOTIC RESISTANCE; BLOOD; EVALUATION STUDY; GENETICS; HOSPITAL EMERGENCY SERVICE; MICROBIOLOGY; MIDDLE AGED; PROCEDURES; SEPSIS; YOUNG ADULT;

ANTIINFECTIVE AGENT;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; ANTI-BACTERIAL AGENTS; BLOOD; DRUG RESISTANCE, BACTERIAL; EMERGENCY SERVICE, HOSPITAL; FEMALE; HUMANS; INTENSIVE CARE UNITS; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; POLYMERASE CHAIN REACTION; SEPSIS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; YOUNG ADULT;

EID: 84949496924     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0140865     Document Type: Article

References (24)

1. O'Brien JM Jr, Ali NA, Aberegg SK, Abraham E. Sepsis. Am J Med. 2007; 120: 1012-1022.
2. Dellinger RP, Levy MM, Rhodes A, Annane D, Gerlach H, Opal SM, et al. Surviving Sepsis Campaign: International Guidelines for Management of Severe Sepsis and Septic Shock: 2012. Crit Care Med. 2013; 41: 580-637. doi: 10.1097/CCM.0b013e31827e83af
3. Kumar A, Ellis P, Arabi Y, Roberts D, Light B, Parrillo JE, et al. Initiation of inappropriate antimicrobial therapy results in a fivefold reduction of survival in human septic shock. Chest. 2009; 136: 1237-1248. doi: 10.1378/chest.09-0087
4. Ecker DJ, Sampath R, Massire C, Blyn LB, Hall TA, Eshoo MW, et al. Ibis T5000: A universal biosensor approach for microbiology. Nat Rev. 2008; 6: 553-558.
5. Ecker DJ, Sampath R, Li H, Massire C, Matthews HE, Toleno D, et al. New technology for rapid molecular diagnosis of bloodstream infections. Expert Rev Mol Diagn. 2010; 10: 399-415. doi: 10.1586/erm. 10.24
6. Jordana-Lluch E, Carolan HE, Giménez M, Sampath R, Ecker DJ, Quesada MD, et al. Rapid diagnosis of bloodstream infections with PCR followed by mass spectrometry. PLoS One. 2013; 8: e62108. doi: 10.1371/journal.pone.0062108
7. Laffler TG, Cummins LL, McClain CM, Quinn CD, Toro MA, Carolan HE, et al. Enhanced Diagnostic Yields of Bacteremia and Candidemia in Blood Specimens by PCR-Electrospray Ionization Mass Spectrometry. J Clin Microbiol. 2013; 51: 3535-3541. doi: 10.1128/JCM.00876-13
8. Bacconi A, Richmond GS, Baroldi MA, Laffler TG, Blyn LB, Carolan HE, et al. Improved Sensitivity for Molecular Detection of Bacteria and Candida in Blood. J Clin Microbiol. 2014; 52:3164-3174 doi: 10. 1128/JCM.00801-14
9. Bone RC, Sibbald WJ, Sprung CL. The ACCP-SCCM consensus conference on sepsis and organ failure. Chest. 1992; 101: 1481-1483.
10. Quesada MD, Gimenez M, Molinos S, Fernandez G, Sanchez MD, Rivelo R, et al. Performance of VITEK-2 Compact and overnight MicroScan panels for direct identification and susceptibility testing of Gram-negative bacilli from positive FAN BacT/ALERT blood culture bottles. Clin Microbiol Infect. 2010; 16:137-140. doi: 10.1111/j.1469-0691.2009.02907.x
11. Giménez M, Quesada M, Molinos S, Colomer M, Hidalgo J, Colomer M, et al. Performance of Vitek-2 compact for direct identificaction and susceptibility testing of Gram-positive coci from positive FAN BacT/ALERT blood culture bottles. (poster number D-1538). 50th Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC), Boston; September 12-15.
12. Dean AG, Sullivan KM, Soe MM. OpenEpi: Open Source Epidemiologic Statistics for Public Health. Available: www.OpenEpi.com, updated 2015/05/04, last accessed 2015/09/02.
13. Mancini N, Carletti S, Ghidoli N, Cichero P, Burioni R, Clementi M. The era of molecular and other nonculture-based methods in diagnosis of sepsis. Clin Microbiol Rev. 2010; 23: 235-251. doi: 10.1128/CMR.00043-09
14. Paolucci M, Landini MP, Sambri V. Conventional and molecular techniques for the early diagnosis of bacteraemia. Int J Antimicrob Agents. 2010; 36 Suppl 2: S6-16. doi: 10.1016/j.ijantimicag.2010.11.010
15. Jordana-Lluch E, Giménez M, Quesada MD, Ausina V, Martró E. Improving the Diagnosis of Bloodstream Infections: PCR Coupled with Mass Spectrometry. Biomed Res Int. 2014; 2014: 1-8.
16. Chang SS, Hsieh WH, Liu TS, Lee SH, Wang CH, Chou HC, et al. Multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis-A systemic review and meta-Analysis. PLoS One. 2013; 8: e62323. doi: 10.1371/journal.pone.0062323
17. Afshari A, Schrenzel J, Ieven M, Harbarth S. Bench-To-bedside review: Rapid molecular diagnostics for bloodstream infection-A new frontier? Crit Care. 2012; 16: 222. doi: 10.1186/cc11202
18. Reimer LG, Wilson ML, Weinstein MP. Update on detection of bacteremia and fungemia. Clin Microbiol Rev. 1997; 10: 444-465.
19. Rangel-Frausto MS, Pittet D, Costigan M, Hwang T, Davis CS, Wenzel RP. The natural history of the systemic inflammatory response syndrome (SIRS). A prospective study. JAMA. 1995; 273: 117-23.
20. Willems E, Smismans A, Cartuyvels R, Coppens G, Van Vaerenbergh K, Van den Abeele AM, et al. The preanalytical optimization of blood cultures: A review and the clinical importance of benchmarking in 5 Belgian hospitals. Diagn Microbiol Infect Dis. 2012; 73: 1-8. doi: 10.1016/j.diagmicrobio.2012.01. 009
21. Szakacs TA, Kalan L, McConnell MJ, Eshaghi A, Shahinas D, McGeer A, et al. Outbreak of vancomycin-susceptible Enterococcus faecium containing the wild-Type vanA gene. J Clin Microbiol. 2014; 52: 1682-6. doi: 10.1128/JCM.03563-13
22. Klare I, Fleige C, Geringer U, Witte W, Werner G. Performance of three chromogenic VRE screening agars, two Etest(1) vancomycin protocols, and different microdilution methods in detecting vanB genotype Enterococcus faecium with varying vancomycin MICs. Diagn Microbiol Infect Dis. 2012; 74: 171-6. doi: 10.1016/j.diagmicrobio.2012.06.020
23. Lehmann L, Herpichboehm B, Kost G, Kollef M, Stuber F. Cost and mortality prediction using polymerase chain reaction pathogen detection in sepsis: evidence from three observational trials. Crit Care. 2010; 14: R186. doi: 10.1186/cc9294
24. Alvarez J, Mar J, Varela-Ledo E, Garea M, Matinez-Lamas L, Rodriguez J, et al. Cost analysis of realtime polymerase chain reaction microbiological diagnosis in patients with septic shock. Anaesth Intensive Care. 2012; 40: 958-63.