Corticosteroid therapy and airflow obstruction influence the bronchial microbiome, which is distinct from that of bronchoalveolar lavage in asthmatic airways

Journal of Allergy and Clinical Immunology

Volumn 137, Issue 5, 2016, Pages 1398-1405.e3

  Denner, Darcy R ^a   Sangwan, Naseer ^b   Becker, Julia B ^a   Hogarth, D Kyle ^a   Oldham, Justin ^a   Castillo, Jamee ^a   Sperling, Anne I ^a   Solway, Julian ^a   Naureckas, Edward T ^a   Gilbert, Jack A ^a,b,c   White, Steven R ^a  

^a UNIVERSITY OF CHICAGO   (United States)

^b ARGONNE NATIONAL LABORATORY   (United States)

^c MARINE BIOLOGICAL LABORATORY   (United States)

Author keywords

16S ribosomal RNA; Asthma; bacteria; corticosteroids; FEV1; microbiome

Indexed keywords

CORTICOSTEROID; DNA; IMMUNOGLOBULIN E; NITRIC OXIDE; RNA 16S; BACTERIAL RNA;

ACIDOBACTERIA; ACTINOBACTERIA; ACTINOMYCES; ADULT; AIRWAY OBSTRUCTION; AMPLICON; ARTICLE; ASTHMA; BACTEROIDES; BACTEROIDETES; BRONCHOALVEOLAR LAVAGE FLUID; BRONCHOSCOPY; COMPARATIVE STUDY; CONTROLLED STUDY; CORTICOSTEROID THERAPY; DNA EXTRACTION; EOSINOPHIL COUNT; EOSINOPHILIA; FEMALE; FIRMICUTES; FORCED EXPIRATORY VOLUME; FUSOBACTERIA; FUSOBACTERIUM; GENE SEQUENCE; HAEMOPHILUS; HUMAN; IMMUNOGLOBULIN BLOOD LEVEL; LACTOBACILLUS; LAVAGE FLUID; LEPTOTRICHIA; LUNG LAVAGE; MAJOR CLINICAL STUDY; MALE; MARINOBACTER; MICROBIAL DIVERSITY; MICROBIOME; NEUTROPHIL COUNT; PREVOTELLA; PRIORITY JOURNAL; PROTEOBACTERIA; PSEUDOMONAS; RICKETTSIA; ROTHIA; SAMPLE; STAPHYLOCOCCUS; STREPTOCOCCUS; VEILLONELLA; BACTERIUM; BRONCHUS; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; MICROFLORA; PATHOPHYSIOLOGY;

ADRENAL CORTEX HORMONES; ADULT; ASTHMA; BACTERIA; BRONCHI; BRONCHOALVEOLAR LAVAGE FLUID; EOSINOPHILIA; FEMALE; FORCED EXPIRATORY VOLUME; HUMANS; MALE; MICROBIOTA; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S;

EID: 84949462578     PISSN: 00916749     EISSN: 10976825     Source Type: Journal    
DOI: 10.1016/j.jaci.2015.10.017     Document Type: Article

References (42)

1. R.P. Baughman, J.E. Thorpe, J. Staneck, M. Rashkin, and P.T. Frame Use of the protected specimen brush in patients with endotracheal or tracheostomy tubes Chest 91 1987 233 236
2. J.M. Beck, V.B. Young, and G.B. Huffnagle The microbiome of the lung Transl Res 160 2012 258 266
3. H. Bisgaard, M.N. Hermansen, F. Buchvald, L. Loland, L.B. Halkjaer, K. Bonnelykke, and et al. Childhood asthma after bacterial colonization of the airway in neonates N Engl J Med 357 2007 1487 1495
4. E.S. Charlson, K. Bittinger, A.R. Haas, A.S. Fitzgerald, I. Frank, A. Yadav, and et al. Topographical continuity of bacterial populations in the healthy human respiratory tract Am J Respir Crit Care Med 184 2011 957 963
5. C. Braun-Fahrlander, J. Riedler, U. Herz, W. Eder, M. Waser, L. Grize, and et al. Environmental exposure to endotoxin and its relation to asthma in school-age children N Engl J Med 347 2002 869 877
6. M.J. Ege, M. Mayer, A.C. Normand, J. Genuneit, W.O. Cookson, C. Braun-Fahrlander, and et al. Exposure to environmental microorganisms and childhood asthma N Engl J Med 364 2011 701 709
7. M.K. Han, Y.J. Huang, J.J. Lipuma, H.A. Boushey, R.C. Boucher, W.O. Cookson, and et al. Significance of the microbiome in obstructive lung disease Thorax 67 2012 456 463
8. F. Blasi, and S.L. Johnston The role of antibiotics in asthma Int J Antimicrob Agents 29 2007 485 493
9. G.G. Brusselle, C. Vanderstichele, P. Jordens, R. Deman, H. Slabbynck, V. Ringoet, and et al. Azithromycin for prevention of exacerbations in severe asthma (AZISAST): A multicentre randomised double-blind placebo-controlled trial Thorax 68 2013 322 329
10. D.L. Hahn, M. Grasmick, S. Hetzel, and S. Yale AZMATICS (AZithroMycin-Asthma Trial In Community Settings) Study Group. Azithromycin for bronchial asthma in adults: An effectiveness trial J Am Board Fam Med 25 2012 442 459
11. J. Altenburg, C.S. de Graaff, T.S. van der Werf, and W.G. Boersma Immunomodulatory effects of macrolide antibiotics - part 1: biological mechanisms Respiration 81 2011 67 74
12. M. Kraft, G.H. Cassell, J. Pak, and R.J. Martin Mycoplasma pneumoniae and Chlamydia pneumoniae in asthma: effect of clarithromycin Chest 121 2002 1782 1788
13. A. Ekici, M. Ekici, and A.K. Erdemoglu Effect of azithromycin on the severity of bronchial hyperresponsiveness in patients with mild asthma J Asthma 39 2002 181 185
14. M. Hilty, C. Burke, H. Pedro, P. Cardenas, A. Bush, C. Bossley, and et al. Disordered microbial communities in asthmatic airways PLoS One 5 2010 e8578
15. P.R. Marri, D.A. Stern, A.L. Wright, D. Billheimer, and F.D. Martinez Asthma-associated differences in microbial composition of induced sputum J Allergy Clin Immunol 131 2013 346 352 e1-3
16. E. Goleva, L.P. Jackson, J.K. Harris, C.E. Robertson, E.R. Sutherland, C.F. Hall, and et al. The effects of airway microbiome on corticosteroid responsiveness in asthma Am J Respir Crit Care Med 188 2013 1193 1201
17. Y.J. Huang, C.E. Nelson, E.L. Brodie, T.Z. Desantis, M.S. Baek, J. Liu, and et al. National Heart, and N. Blood Institute's Asthma Clinical Research, Airway microbiota and bronchial hyperresponsiveness in patients with suboptimally controlled asthma J Allergy Clin Immunol 127 2011 372 381 e1-3
18. J. Nicodemus-Johnson, B. Laxman, R.K. Stern, J. Sudi, C.N. Tierney, L. Norwick, and et al. Maternal asthma and microRNA regulation of soluble HLA-G in the airway J Allergy Clin Immunol 131 2013 1496 1503
19. S.R. White, D.A. Loisel, J.F. McConville, R. Stern, Y. Tu, B.A. Marroquin, and et al. Levels of soluble human leukocyte antigen-G are increased in asthmatic airways Eur Respir J 35 2010 925 927
20. R.C. Edgar UPARSE: highly accurate OTU sequences from microbial amplicon reads Nat Methods 10 2013 996 998
21. J.G. Caporaso, J. Kuczynski, J. Stombaugh, K. Bittinger, F.D. Bushman, E.K. Costello, and et al. QIIME allows analysis of high-throughput community sequencing data Nat Methods 7 2010 335 336
22. P.J. McMurdie Holmes S. phyloseq: An R package for reproducible interactive analysis and graphics of microbiome census data PLoS One 8 2013 e61217
23. J.N. Paulson, O.C. Stine, H.C. Bravo, and M. Pop Differential abundance analysis for microbial marker-gene surveys Nat Methods 10 2013 1200 1202
24. S.E. Wenzel, S.J. Szefler, D.Y. Leung, S.I. Sloan, M.D. Rex, and R.J. Martin Bronchoscopic evaluation of severe asthma. Persistent inflammation associated with high dose glucocorticoids Am J Respir Crit Care Med 156 1997 737 743
25. S.E. Wenzel, L.B. Schwartz, E.L. Langmack, J.L. Halliday, J.B. Trudeau, R.L. Gibbs, and et al. Evidence that severe asthma can be divided pathologically into two inflammatory subtypes with distinct physiologic and clinical characteristics Am J Respir Crit Care Med 160 1999 1001 1008
26. J.V. Fahy, K.W. Kim, J. Liu, and H.A. Boushey Prominent neutrophilic inflammation in sputum from subjects with asthma exacerbation J Allergy Clin Immunol 95 1995 843 852
27. P.G. Woodruff, R. Khashayar, S.C. Lazarus, S. Janson, P. Avila, H.A. Boushey, and et al. Relationship between airway inflammation, hyperresponsiveness, and obstruction in asthma J Allergy Clin Immunol 108 2001 753 758
28. S.A. Little, K.J. MacLeod, G.W. Chalmers, J.G. Love, C. McSharry, and N.C. Thomson Association of forced expiratory volume with disease duration and sputum neutrophils in chronic asthma Am J Med 112 2002 446 452
29. M.L. Walker, K.E. Holt, G.P. Anderson, S.M. Teo, P.D. Sly, P.G. Holt, and et al. Elucidation of pathways driving asthma pathogenesis: development of a systems-level analytic strategy Front Immunol 5 2014 447
30. B.D. Spycher, M. Silverman, and C.E. Kuehni Phenotypes of childhood asthma: Are they real? Clin Exp Allergy 40 2010 1130 1141
31. E.S. Charlson, J.M. Diamond, K. Bittinger, A.S. Fitzgerald, A. Yadav, A.R. Haas, and et al. Lung-enriched organisms and aberrant bacterial and fungal respiratory microbiota after lung transplant Am J Respir Crit Care Med 186 2012 536 545
32. E.S. Charlson, K. Bittinger, J. Chen, J.M. Diamond, H. Li, R.G. Collman, and et al. Assessing bacterial populations in the lung by replicate analysis of samples from the upper and lower respiratory tracts PLoS One 7 2012 e42786
33. L.N. Segal, A.V. Alekseyenko, J.C. Clemente, R. Kulkarni, B. Wu, Z. Gao, and et al. Enrichment of lung microbiome with supraglottic taxa is associated with increased pulmonary inflammation Microbiome 1 2013 19
34. Expert Panel Report 3 (EPR-3): guidelines for the diagnosis and management of asthma - summary report 2007 J Allergy Clin Immunol 120 suppl 2007 S94 S138
35. R.O. Crapo, R. Casaburi, A.L. Coates, P.L. Enright, J.L. Hankinson, C.G. Irvin, and et al. Guidelines for methacholine and exercise challenge testing-1999. This official statement of the American Thoracic Society was adopted by the ATS Board of Directors, July 1999 Am J Respir Crit Care Med 161 2000 309 329
36. P.J. McMurdie Holmes S. phyloseq: An R package for reproducible interactive analysis and graphics of microbiome census data PLoS One 8 2013 e61217
37. J.N. Paulson, O.C. Stine, H.C. Bravo, and M. Pop Differential abundance analysis for microbial marker-gene surveys Nat Methods 10 2013 1200 1202
38. R.C. Edgar UPARSE: highly accurate OTU sequences from microbial amplicon reads Nat Methods 10 2013 996 998
39. J.G. Caporaso, J. Kuczynski, J. Stombaugh, K. Bittinger, F.D. Bushman, E.K. Costello, and et al. QIIME allows analysis of high-throughput community sequencing data Nat Methods 7 2010 335 336
40. P.D. Schloss, S.L. Westcott, T. Ryabin, J.R. Hall, M. Hartmann, E.B. Hollister, and et al. Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities Appl Environ Microbiol 75 2009 7537 7541
41. C. Quast, E. Pruesse, P. Yilmaz, J. Gerken, T. Schweer, P. Yarza, and et al. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools Nucleic Acids Res 41 2013 D590 D596
42. J.R. White, N. Nagarajan, and M. Pop Statistical methods for detecting differentially abundant features in clinical metagenomic samples PLoS Comput Biol 5 2009 e1000352