TALEN- and CRISPR/Cas9-mediated gene editing in human pluripotent stem cells using lipid-based transfection

Current Protocols in Stem Cell Biology

Volumn 2015, Issue , 2015, Pages 5B.3.1-5B.3.25

  Hendriks, William T ^a   Jiang, Xin ^b   Daheron, Laurence ^b   Cowan, Chad A ^b,c  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD UNIVERSITY   (United States)

^c MASSACHUSETTS GENERAL HOSPITAL CANCER CENTER   (United States)

Author keywords

CRISPR Cas9; Gene editing; Pluripotent stem cells; TALEN; Transfection

Indexed keywords

CRISPR ASSOCIATED PROTEIN; LIPID; NUCLEASE; OLIGODEOXYNUCLEOTIDE DERIVATIVE; PROTEIN CAS9; RNA; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; UNCLASSIFIED DRUG; DEOXYRIBONUCLEASE;

ARTICLE; DOWNSTREAM PROCESSING; ENZYME ACTIVITY; GENE CONSTRUCT; GENE VECTOR; GENETIC ENGINEERING; GENETIC SELECTION; GENETIC TRANSFECTION; GENOTYPE; HUMAN; HUMAN CELL; MOLECULAR CLONING; PLURIPOTENT STEM CELL; POINT MUTATION; PRIORITY JOURNAL; RNA EDITING; CRISPR CAS SYSTEM; CYTOLOGY; GENETICS; METABOLISM; PROCEDURES;

CRISPR-CAS SYSTEMS; DEOXYRIBONUCLEASES; GENETIC VECTORS; HUMANS; PLURIPOTENT STEM CELLS; TRANSFECTION;

EID: 84949212657     PISSN: 19417322     EISSN: 19388969     Source Type: Journal    
DOI: 10.1002/9780470151808.sc05b03s34     Document Type: Article

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