Reducing time to identification of positive blood cultures with MALDI-TOF MS analysis after a 5-h subculture

European Journal of Clinical Microbiology and Infectious Diseases

Volumn 34, Issue 2, 2015, Pages 405-413

  Verroken, A ^a,b   Defourny, L ^b   Lechgar, L ^b   Magnette, A ^b   Delmee M ^a,b   Glupczynski, Y ^c  

^a UNIVERSITÉ CATHOLIQUE DE LOUVAIN   (Belgium)

^b CLINIQUES UNIVERSITAIRES SAINT LUC   (Belgium)

^c UNIVERSITY HOSPITAL OF MONT GODINNE   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACILLUS CEREUS; BACTERIAL GROWTH; BACTERIUM CONTAMINATION; BLOOD CULTURE; BLOODSTREAM INFECTION; CORYNEBACTERIUM; CORYNEBACTERIUM JEIKEIUM; ENTEROBACTER AEROGENES; ENTEROBACTER CLOACAE; ENTEROBACTERIACEAE; ENTEROCOCCUS; ENTEROCOCCUS AVIUM; ENTEROCOCCUS FAECALIS; ENTEROCOCCUS FAECIUM; ESCHERICHIA COLI; GENE SEQUENCE; GORDONIA; GRANULICATELLA ADIACENS; HAEMOPHILUS INFLUENZAE; HAFNIA ALVEI; HUMAN; KLEBSIELLA OXYTOCA; KLEBSIELLA PNEUMONIAE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MICROCOCCUS LUTEUS; MORGANELLA MORGANII; NEISSERIA MENINGITIDIS; PRIORITY JOURNAL; PROTEUS MIRABILIS; SPECIES IDENTIFICATION; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS CAPITIS; STAPHYLOCOCCUS COHNII; STAPHYLOCOCCUS EPIDERMIDIS; STAPHYLOCOCCUS HAEMOLYTICUS; STAPHYLOCOCCUS HOMINIS; STAPHYLOCOCCUS LUGDUNENSIS; STAPHYLOCOCCUS SCIURI; STAPHYLOCOCCUS WARNERI; STREPTOCOCCUS; STREPTOCOCCUS AGALACTIAE; STREPTOCOCCUS ANGINOSUS; STREPTOCOCCUS DYSGALACTIAE; STREPTOCOCCUS MITIS; STREPTOCOCCUS PNEUMONIAE; STREPTOCOCCUS PYOGENES; STREPTOCOCCUS SALIVARIUS; STREPTOCOCCUS SANGUINIS; BACTEREMIA; CULTURE MEDIUM; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; MASS SPECTROMETRY; MICROBIOLOGY; PROCEDURES; SEPSIS; TIME FACTOR; VALIDATION STUDY;

CULTURE MEDIUM; FORMIC ACID; FORMIC ACID DERIVATIVE;

BACTEREMIA; CULTURE MEDIA; FORMATES; GRAM-NEGATIVE BACTERIA; GRAM-POSITIVE BACTERIA; HUMANS; MASS SPECTROMETRY; SEPSIS; SPECIMEN HANDLING; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TIME FACTORS;

EID: 84922005299     PISSN: 09349723     EISSN: 14354373     Source Type: Journal    
DOI: 10.1007/s10096-014-2242-4     Document Type: Article

References (30)

1. Beekmann SE, Diekema DJ, Chapin KC, Doern GV (2003) Effects of rapid detection of bloodstream infections on length of hospitalization and hospital charges. J Clin Microbiol 41:3119–3125
2. Galar A, Leiva J, Espinosa M, Guillén-Grima F, Hernáez S, Yuste JR (2012) Clinical and economic evaluation of the impact of rapid microbiological diagnostic testing. J Infect 65:302–309
3. Stoneking LR, Patanwala AE, Winkler JP, Fiorello AB, Lee ES, Olson DP, Wolk DM (2013) Would earlier microbe identification alter antibiotic therapy in bacteremic emergency department patients? J Emerg Med 44:1–8
4. Peters RPH, van Agtmael MA, Danner SA, Savelkoul PHM, Vandenbroucke-Grauls CMJE (2004) New developments in the diagnosis of bloodstream infections. Lancet Infect Dis 4:751–760
5. Chang SS, Hsieh WH, Liu TS, Lee SH, Wang CH, Chou HC, Yeo YH, Tseng CP, Lee CC (2013) Multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis—a systemic review and meta-analysis. PLoS One 8(5):e62323. doi:10.1371/journal.pone.0062323
6. Seng P, Drancourt M, Gouriet F, La Scola B, Fournier PE, Rolain JM, Raoult D (2009) Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis 49:543–551
7. Ferroni A, Suarez S, Beretti JL, Dauphin B, Bille E, Meyer J, Bougnoux ME, Alanio A, Berche P, Nassif X (2010) Real-time identification of bacteria and Candida species in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 48:1542–1548
8. Fuglsang-Damgaard D, Houlberg Nielsen C, Mandrup E, Fuursted K (2011) The use of Gram stain and matrix-assisted laser desorption ionization time-of-flight mass spectrometry on positive blood culture: synergy between new and old technology. APMIS 119:681–688
9. Loonen AJM, Jansz AR, Stalpers J, Wolffs PFG, van den Brule AJC (2012) An evaluation of three processing methods and the effect of reduced culture times for faster direct identification of pathogens from BacT/ALERT blood cultures by MALDI-TOF MS. Eur J Clin Microbiol Infect Dis 31:1575–1583
10. Martiny D, Dediste A, Vandenberg O (2012) Comparison of an in-house method and the commercial Sepsityper™ kit for bacterial identification directly from positive blood culture broths by matrix-assisted laser desorption-ionisation time-of-flight mass spectrometry. Eur J Clin Microbiol Infect Dis 31:2269–2281
11. Buchan BW, Riebe KM, Ledeboer NA (2012) Comparison of the MALDI Biotyper system using Sepsityper specimen processing to routine microbiological methods for identification of bacteria from positive blood culture bottles. J Clin Microbiol 50:346–352
12. Meex C, Neuville F, Descy J, Huynen P, Hayette MP, De Mol P, Melin P (2012) Direct identification of bacteria from BacT/ALERT anaerobic positive blood cultures by MALDI-TOF MS: MALDI Sepsityper kit versus an in-house saponin method for bacterial extraction. J Med Microbiol 61:1511–1516
13. Romero-Gómez MP, Gómez-Gil R, Paño-Pardo JR, Mingorance J (2012) Identification and susceptibility testing of microorganism by direct inoculation from positive blood culture bottles by combining MALDI-TOF and Vitek-2 Compact is rapid and effective. J Infect 65:513–520
14. Chen JHK, Ho PL, Kwan GSW, She KKK, Siu GKH, Cheng VCC, Yuen KY, Yam WC (2013) Direct bacterial identification in positive blood cultures by use of two commercial matrix-assisted laser desorption ionization-time of flight mass spectrometry systems. J Clin Microbiol 51:1733–1739
15. Leli C, Cenci E, Cardaccia A, Moretti A, D’Alò F, Pagliochini R, Barcaccia M, Farinelli S, Vento S, Bistoni F, Mencacci A (2013) Rapid identification of bacterial and fungal pathogens from positive blood cultures by MALDI-TOF MS. Int J Med Microbiol 303:205–209
16. Martiny D, Debaugnies F, Gateff D, Gérard M, Aoun M, Martin C, Konopnicki D, Loizidou A, Georgala A, Hainaut M, Chantrenne M, Dediste A, Vandenberg O, Van Praet S (2013) Impact of rapid microbial identification directly from positive blood cultures using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on patient management. Clin Microbiol Infect 19:E568–E581
17. Vlek ALM, Bonten MJM, Boel CHE (2012) Direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry improves appropriateness of antibiotic treatment of bacteremia. PLoS One 7(3):e32589. doi:10.1371/journal.pone.0032589
18. De Bel A, Wybo I, Piérard D, Lauwers S (2010) Correct implementation of matrix-assisted laser desorption ionization-time of flight mass spectrometry in routine clinical microbiology. J Clin Microbiol 48:1991–1992
19. Wauters G, Avesani V, Laffineur K, Charlier J, Janssens M, Van Bosterhaut B, Delmée M (2003) Brevibacterium lutescens sp. nov., from human and environmental samples. Int J Syst Evol Microbiol 53:1321–1325
20. Centers for Disease Control and Prevention/National Healthcare Safety Network (CDC/NHSN) (2014) CDC/NHSN surveillance definitions for specific types of infections. Available online at: http://www.cdc.gov/nhsn/pdfs/pscmanual/17pscnosinfdef_current.pdf. Accessed 19 June 2014
21. McElvania TeKippe E, Shuey S, Winkler DW, Butler MA, Burnham CAD (2013) Optimizing identification of clinically relevant Gram-positive organisms by use of the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry system. J Clin Microbiol 51:1421–1427
22. Ford BA, Burnham CAD (2013) Optimization of routine identification of clinically relevant Gram-negative bacteria by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and the Bruker Biotyper. J Clin Microbiol 51:1412–1420
23. Idelevich EA, Schüle I, Grünastel B, Wüllenweber J, Peters G, Becker K (2014) Rapid identification of microorganisms from positive blood cultures by MALDI-TOF mass spectrometry subsequent to very short-term incubation on solid medium. Clin Microbiol Infect. doi:10.1111/1469-0691.12640
24. Mestas J, Felsenstein S, Dien Bard J (2014) Direct identification of bacteria from positive BacT/ALERT blood culture bottles using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. Diagn Microbiol Infect Dis. doi:10.1016/j.diagmicrobio.2014.07.008
25. Bassetti M, Merelli M, Righi E, Diaz-Martin A, Rosello EM, Luzzati R, Parra A, Trecarichi EM, Sanguinetti M, Posteraro B, Garnacho-Montero J, Sartor A, Rello J, Tumbarello M (2013) Epidemiology, species distribution, antifungal susceptibility, and outcome of candidemia across five sites in Italy and Spain. J Clin Microbiol 51:4167–4172
26. Ngo JT, Parkins MD, Gregson DB, Pitout JD, Ross T, Church DL, Laupland KB (2013) Population-based assessment of the incidence, risk factors, and outcomes of anaerobic bloodstream infections. Infection 41:41–48
27. Pulcrano G, Iula DV, Vollaro A, Tucci A, Cerullo M, Esposito M, Rossano F, Catania MR (2013) Rapid and reliable MALDI-TOF mass spectrometry identification of Candida non-albicans isolates from bloodstream infections. J Microbiol Methods 94:262–266
28. Neville SA, LeCordier A, Ziochos H, Chater MJ, Gosbell IB, Maley MW, van Hal SJ (2011) Utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry following introduction for routine laboratory bacterial identification. J Clin Microbiol 49:2980–2984
29. Kärpänoja P, Harju I, Rantakokko-Jalava K, Haanperä M, Sarkkinen H (2014) Evaluation of two matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for identification of viridans group streptococci. Eur J Clin Microbiol Infect Dis 33:779–788. doi:10.1007/s10096-013-2012-8
30. European Centre for Disease Prevention and Control (ECDC) (2013) Surveillance report: antimicrobial resistance surveillance in Europe 2012. Available online at: http://www.ecdc.europa.eu/en/publications/Publications/antimicrobial-resistance-surveillance-europe-2012.pdf. Accessed 6 July 2014