Small-scale recombinant adeno-associated virus purification

Methods in Molecular Biology

Volumn 1382, Issue , 2016, Pages 95-106

  Burger, Corinna ^a   Nash, Kevin R ^a  

^a NONE

Author keywords

Adeno associated virus; Iodixanol gradient purification; Tissue culture

Indexed keywords

PARVOVIRUS VECTOR; IODIXANOL; IODOBENZOIC ACID DERIVATIVE;

ADENO ASSOCIATED VIRUS; CHROMATOGRAPHY; GENE EXPRESSION; HEK293 CELL LINE; HUMAN; HUMAN CELL; NONHUMAN; SEROTYPE; ULTRACENTRIFUGATION; VIRAL GENE DELIVERY SYSTEM; VIRAL GENE THERAPY; VIRUS CAPSID; VIRUS GENOME; VIRUS PURIFICATION; VIRUS RECOMBINANT; CHEMISTRY; DEPENDOPARVOVIRUS; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; VIRUS LOAD;

DEPENDOVIRUS; GENETIC VECTORS; HEK293 CELLS; HUMANS; TRIIODOBENZOIC ACIDS; VIRAL LOAD;

EID: 84948799176     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-3271-9_7     Document Type: Chapter

References (11)

1. Ayuso E, Mingozzi F, Bosch F (2010) Production, purifi cation and characterization of adeno-associated vectors. Curr Gene Ther 10(6):423–436
2. Virag T, Cecchini S, Kotin RM (2009) Producing recombinant adeno-associated virus in foster cells: overcoming production limitations using a baculovirus-insect cell expression strategy. Hum Gene Ther 20(8):807–817
3. Carter BJ, Antoni BA, Klessig DF (1992) Adenovirus containing a deletion of the early region 2A gene allows growth of adenoassociated virus with decreased effi ciency. Virology 191:473–476
4. Ward P et al (1998) Role of the adenovirus DNAbinding protein in in vitro adeno- associated virus DNA replication. J Virol 72(1):420–427
5. Janik JE et al (1989) Effi cient synthesis of adeno-associated virus structural proteins requires both adenovirus DNA binding protein and VA I RNA. Virology 168:320–329
6. Chang LS, Shenk T (1990) The adenovirus DNA-binding protein stimulates the rate of transcription directed by adenovirus and adeno-associated virus promoters. J Virol 64: 2103–2109
7. Nash K, Chen W, Muzyczka N (2008) Complete in vitro reconstitution of adenoassociated virus DNA replication requires the minichromosome maintenance complex proteins. J Virol 82(3):1458–1464
8. Zolotukhin S et al (1999) Recombinant adenoassociated virus purifi cation using novel methods improves infectious titer and yield. Gene Ther 6(6):973–985
9. Binny CJ, Nathwani AC (2012) Vector systems for prenatal gene therapy: principles of adenoassociated virus vector design and production. Methods Mol Biol 891:109–131
10. Chahal PS et al (2014) Production of adenoassociated virus (AAV) serotypes by transient transfection of HEK293 cell suspension cultures for gene delivery. J Virol Methods 196: 163–173
11. Zolotukhin S et al (2002) Production and purifi cation of serotype 1, 2, and 5 recombinant adeno-associated viral vectors. Methods 28(2): 158–167