Comparison of 7 published LC-MS/MS methods for the simultaneous measurement of testosterone, androstenedione, and dehydroepiandrosterone in serum

Clinical Chemistry

Volumn 61, Issue 12, 2015, Pages 1475-1483

  Büttler, Rahel M ^a   Martens, Frans ^a   Fanelli, Flaminia ^b   Pham, Hai T ^c   Kushnir, Mark M ^d,e   Janssen, Marcel J W ^f   Owen, Laura ^g   Taylor, Angela E ^h   Soeborg, Tue ⁱ   Blankenstein, Marinus A ^a   Heijboer, Annemieke C ^a  

^a VU UNIVERSITY MEDICAL CENTER   (Netherlands)

^b UNIVERSITY OF BOLOGNA   (Italy)

^c BIOCRATES LIFE SCIENCES AG   (Austria)

^d UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

^e ARUP INSTITUTE FOR CLINICAL AND EXPERIMENTAL PATHOLOGY   (United States)

^f Department of Obstetrics and Gynaecology   (Netherlands)

^g MANCHESTER UNIVERSITY NHS FOUNDATION TRUST   (United Kingdom)

^h UNIVERSITY OF BIRMINGHAM   (United Kingdom)

ⁱ University of Copenhagen   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ANDROSTENEDIONE; PRASTERONE; TESTOSTERONE;

ANDROSTENEDIONE BLOOD LEVEL; ARTICLE; CONTROLLED STUDY; CORRELATION COEFFICIENT; FEMALE; HORMONE DETERMINATION; HUMAN; IMMUNOASSAY; INTERMETHOD COMPARISON; LIMIT OF QUANTITATION; LIQUID CHROMATOGRAPHY; MAJOR CLINICAL STUDY; MALE; RANDOM SAMPLE; TANDEM MASS SPECTROMETRY; TESTOSTERONE BLOOD LEVEL; ADULT; BLOOD; CALIBRATION; COMPARATIVE STUDY; OBSERVER VARIATION; REGRESSION ANALYSIS; REPRODUCIBILITY; SEX DIFFERENCE; STANDARDS;

ADULT; ANDROSTENEDIONE; CALIBRATION; CHROMATOGRAPHY, LIQUID; DEHYDROEPIANDROSTERONE; FEMALE; HUMANS; MALE; OBSERVER VARIATION; REGRESSION ANALYSIS; REPRODUCIBILITY OF RESULTS; SEX FACTORS; TANDEM MASS SPECTROMETRY; TESTOSTERONE;

EID: 84948688988     PISSN: 00099147     EISSN: 15308561     Source Type: Journal    
DOI: 10.1373/clinchem.2015.242859     Document Type: Article

References (19)

1. Taieb J, Mathian B, Millot F et al. Testosterone measured by 10 immunoassays and by isotope-dilution gas chromatography-mass spectrometry in sera from 116 men, women, and children. Clin Chem 2003;49:1381-95.
2. Herold DA, Fitzgerald RL. Immunoassays for testosterone in women: better than a guess? Clin Chem 2003; 49:1250-1.
3. Rosner W, Auchus RJ, Azziz R, Sluss PM, Raff H. Utility, limitations, and pitfalls in measuring testosterone: an Endocrine Society position statement. J Clin Endocrinol Metab 2007;92:405-13.
4. Rosner W, Vesper H. Toward excellence in testosterone testing: a consensus statement. J Clin Endocrinol Metab 2010;95:4542-8.
5. Ackermans MT, Endert E. LC-MS/MS in endocrinology: what is the profit of the last 5 years? Bioanalysis 2014; 6:43-57.
6. Handelsman DJ, Wartofsky L. Requirement for mass spectrometry sex steroid assays in the Journal of Clinical Endocrinology and Metabolism. J Clin Endocrinol Metab 2013;98:3971-3.
7. Thienpont LM, Van Uytfanghe K, Blincko S et al. Stateof-the-art of serum testosterone measurement by isotope dilution-liquid chromatography-tandem mass spectrometry. Clin Chem 2008;54:1290-7.
8. Vesper HW, Bhasin S,WangC et al. Interlaboratory comparison study of serum total testosterone measurements performed by mass spectrometry methods. Steroids 2009;74:498-503.
9. Legro RS, Schlaff WD, Diamond MP et al. Total testosterone assays in women with polycystic ovary syndrome: precision and correlation with hirsutism. J Clin Endocrinol Metab 2010;95:5305-13.
10. Büttler RM, Martens F, Kushnir MM, Ackermans MT, Blankenstein MA, Heijboer AC. Simultaneous measurement of testosterone, androstenedione and dehydroepiandrosterone (DHEA) in serum and plasma with isotope-dilution 2-dimension ultra high performance liquid-chromatography tandem mass spectrometry (ID-LC-MS/MS). Clin Chim Acta 2015;438:157-9.
11. Groenestege WMT, Bui HN, ten Kate J et al. Accuracy of first and second generation testosterone assays and improvement through sample extraction. Clin Chem 2012;58:1154-6.
12. Bui HN, Sluss PM, Blincko S, Knol DL, Blankenstein MA, Heijboer AC. Dynamics of serum testosterone during the menstrual cycle evaluated by daily measurements with an ID-LC-MS/MS method and a 2nd generation automated immunoassay. Steroids 2013;78:96-101.
13. Shi RZ, van Rossum HH, Bowen RAR. Serum testosterone quantitation by liquid chromatography-tandem mass spectrometry: interference from blood collection tubes. Clin Biochem 2012;45:1706-9.
14. Fanelli F, Belluomo I, Di Lallo VD et al. Serum steroid profiling by isotopic dilution-liquid chromatographymass spectrometry: comparison with current immunoassays and reference intervals in healthy adults. Steroids 2011;76:244-53.
15. Münzker J, Hofer D, Trummer C et al. Testosterone to dihydrotestosterone ratio as a new biomarker for an adverse metabolic phenotype in the polycystic ovary syndrome. J Clin Endocrinol Metab 2015;100:653-60.
16. Koal T, Schmiederer D, Pham-Tuan H, Rohring C, Rauh M. Standardized LC-MS/MS based steroid hormone profile-analysis. J Steroid Biochem Mol Biol 2011;129: 129-38.
17. Kushnir MM, Blamires T, Rockwood AL et al. Liquid chromatography-tandem mass spectrometry assay for androstenedione, dehydroepiandrosterone, and testosterone with pediatric and adult reference intervals. Clin Chem 2010;56:1138-47.
18. O'Reilly MW, Taylor AE, Crabtree NJ et al. Hyperandrogenemia predicts metabolic phenotype in polycystic ovary syndrome: the utility of serum androstenedione. J Clin Endocrinol Metab 2014;99:1027-36.
19. Soeborg T, Frederiksen H, Fruekilde P, Johannsen TH, Juul A, Andersson AM. Serum concentrations of DHEA, DHEAS, 17alpha-hydroxyprogesterone, Delta4-androstenedione and testosterone in children determined by TurboFlow-LC-MS/MS. Clin Chim Acta 2013;419:95-101.