A single-cell assay for telomere DNA content shows increasing telomere length heterogeneity, as well as increasing mean telomere length in human spermatozoa with advancing age

Journal of Assisted Reproduction and Genetics

Volumn 32, Issue 11, 2015, Pages 1685-1690

  Antunes, Danielle M F ^a,b   Kalmbach, Keri H ^a   Wang, Fang ^a   Dracxler, Roberta C ^c   Seth Smith, Michelle L ^a   Kramer, Yael ^a   Buldo Licciardi, Julia ^a   Kohlrausch, Fabiana B ^b   Keefe, David L ^a,c  

^a NEW YORK UNIVERSITY MEDICAL CENTER   (United States)

^b FLUMINENSE FEDERAL UNIVERSITY   (Brazil)

^c NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Human spermatozoa; In vitro fertilization; Paternal age; Sperm morphology; Telomeres

Indexed keywords

DNA;

ADULT; AGING; ARTICLE; ASTHENOSPERMIA; CELL ASSAY; CROSS-SECTIONAL STUDY; DNA CONTENT; FERTILIZATION IN VITRO; HUMAN; INTRACYTOPLASMIC SPERM INJECTION; MAJOR CLINICAL STUDY; MALE; OLIGOASTHENOZOOSPERMIA; OLIGOSPERMIA; PILOT STUDY; PRIORITY JOURNAL; SEMEN ABNORMALITY; SEMEN ANALYSIS; SINGLE CELL ASSAY; SPERMATOGENESIS; SPERMATOZOON; SPERMATOZOON DENSITY; TELOMERE HOMEOSTASIS; GENETICS; MIDDLE AGED; PATERNAL AGE; PHYSIOLOGY; PROCEDURES; PROSPECTIVE STUDY; SINGLE CELL ANALYSIS; TELOMERE;

ADULT; CROSS-SECTIONAL STUDIES; DNA; HUMANS; MALE; MIDDLE AGED; PATERNAL AGE; PROSPECTIVE STUDIES; SINGLE-CELL ANALYSIS; SPERMATOZOA; TELOMERE; TELOMERE HOMEOSTASIS;

EID: 84947485771     PISSN: 10580468     EISSN: 15737330     Source Type: Journal    
DOI: 10.1007/s10815-015-0574-3     Document Type: Article

References (33)

1. Blackburn EH. Telomere states and cell fates. Nature. 2000;408:53–6.
2. Liu L et al. An essential role for functional telomeres in mouse germ cells during fertilization and early development. Dev Biol. 2002;249:74–84.
3. Keefe DL et al. Telomere length predicts embryo fragmentation after in vitro fertilization in women—toward a telomere theory of reproductive aging in women. Am J Obst Gynecol. 2005;192:1256–60.
4. Sahin E et al. Telomere dysfunction induces metabolic and mitochondrial compromise. Nature. 2011;470:359–65.
5. Schaetzlein S et al. Telomere length is reset during early mammalian embryogenesis. Proc Natl Acad Sci U S A. 2004;101:8034–8.
6. Kalmbach KH et al. Telomeres and human reproduction. Fertil Steril. 2013;99:23–9.
7. Krawetz SA. Paternal contribution: new insights and future challenges. Nat Rev Genet. 2005;6:633–42.
8. Moskovtsev SI et al. Disruption of telomere-telomere interactions associated with DNA damage in human spermatozoa. Syst Biol Reprod Med. 2010;56:407–12.
9. Kimura M et al. Offspring’s leukocyte telomere length, paternal age, and telomere elongation in sperm. PLoS Genet. 2008;4, e37.
10. Ioannou D, Griffin DK. Male fertility, chromosome abnormalities, and nuclear organization. Cytogenet Genome Res. 2011;133:269–79.
11. Lee HW et al. Essential role of mouse telomerase in highly proliferative organs. Nature. 1998;392:569–74.
12. Reig-Viader R et al. Telomeric repeat-containing RNA (TERRA) and telomerase are components of telomeres during mammalian gametogenesis. Biol Reprod. 2014;90(5):103.
13. Reig-Viader R et al. Telomere homeostasis is compromised in spermatocytes from patients with idiopathic infertility. Fertil Steril. 2014;102(3):728–38.
14. Allsopp RC et al. Telomere length predicts replicative capacity of human fibroblasts. Proc Natl Acad Sci U S A. 1992;89:10114–8.
15. Aston KI et al. Divergence of sperm and leukocyte age-dependent telomere dynamics: implications for male-driven evolution of telomere length in humans. Mol Hum Reprod. 2012;18:517–22.
16. Baird DM et al. Telomere instability in the male germline. Hum Mol Genet. 2006;15:45–51.
17. Sartorius GA, Nieschlag E. Paternal age and reproduction. Hum Reprod Update. 2010;16:65–79.
18. Bryan TM et al. The telomere lengthening mechanism in telomerase-negative immortal human cells does not involve the telomerase RNA subunit. Hum Mol Genet. 1997;6:921–6.
19. Cesare AJ, Reddel RR. Alternative lengthening of telomeres: models, mechanisms and implications. Nat Rev Genet. 2010;11:319–30.
20. Dolcetti R, De Rossi A. Telomere/telomerase interplay in virus-driven and virus-independent lymphomagenesis: pathogenic and clinical implications. Med Res Rev. 2012;32:233–53.
21. Bryan TM et al. Telomere elongation in immortal human cells without detectable telomerase activity. EMBO J. 1995;14:4240–8.
22. Liu L et al. Telomere lengthening early in development. Nat Cell Biol. 2007;9:1436–41.
23. Li B, Lustig AJ. A novel mechanism for telomere size control in Saccharomyces cerevisiae. Genes Dev. 1996;10:1310–26.
24. Bucholc M, Park Y, Lustig AJ. Intrachromatid excision of telomeric DNA as a mechanism for telomere size control in Saccharomyces cerevisiae. Mol Cell Biol. 2001;21:6559–73.
25. von Zglinicki T, Saretzki G, Docke W, Lotze C. Mild hyperoxia shortens telomeres and inhibits proliferation of fibroblasts: a model for senescence? Exp Cell Res. 1995;220:186–93.
26. Aitken RJ et al. Oxidative stress and male reproductive health. Asian J Androl. 2014;16:31–8.
27. Thilagavathi J et al. Analysis of sperm telomere length in men with idiopathic infertility. Arch Gynecol Obstet. 2013;287:803–7.
28. Ferlin A et al. In young men sperm telomere length is related to sperm number and parental age. Hum Reprod. 2013;28:3370–6.
29. World Health Organization. WHO laboratory manual for the examination and processing of human semen. 2010; 5th edition
30. Wang F et al. Robust measurement of telomere length in single cells. Proc Natl Acad Sci U S A. 2013;110:E1906–12.
31. Nordstokke DW, Zumbo BD. A new nonparametric Levene test for equal variances. Psicológica. 2010;31:401–30.
32. Turner S, Hartshorne GM. Telomere lengths in human pronuclei, oocytes and spermatozoa. Mol Hum Reprod. 2013;19:510–8.
33. Blackburn EH, Greider CW, Szostak JW. Telomeres and telomerase: the path from maize, Tetrahymena and yeast to human cancer and aging. Nat Med. 2006;12:1133–8.