A dual control mechanism synchronizes riboflavin and sulphur metabolism in Bacillus subtilis

Proceedings of the National Academy of Sciences of the United States of America

Volumn 112, Issue 45, 2015, Pages 14054-14059

  Pedrolli, Danielle Biscaro ^a,b   Kühm, Christian ^a   Sévin, Daniel C ^c   Vockenhuber, Michael P ^d   Sauer, Uwe ^c   Suess, Beatrix ^d   Mack, Matthias ^a  

^a MANNHEIM UNIVERSITY OF APPLIED SCIENCES   (Germany)

^b SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

^c ETH ZURICH   (Switzerland)

^d DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Bacillus subtilis; Flavin mononucleotide; FMN riboswitch; Riboflavin; RibR

Indexed keywords

APTAMER; FLAVINE MONONUCLEOTIDE; METHIONINE; RIBOFLAVIN; RIBOFLAVIN SYNTHASE; SULFUR; TAURINE; RECOMBINANT PROTEIN; RIBOSWITCH;

ARTICLE; BACILLUS SUBTILIS; CONTROLLED STUDY; DNA TEMPLATE; ENZYME ACTIVITY; GEL MOBILITY SHIFT ASSAY; GENE EXPRESSION; IN VITRO STUDY; METABOLIC REGULATION; NONHUMAN; PRIORITY JOURNAL; REPORTER GENE; RIBOSWITCH; RNA BINDING; SYNTHESIS; TIME OF FLIGHT MASS SPECTROMETRY; TRANSCRIPTION TERMINATION; WESTERN BLOTTING; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METABOLOME; PHYSIOLOGY;

BACILLUS SUBTILIS; ELECTROPHORETIC MOBILITY SHIFT ASSAY; FLAVIN MONONUCLEOTIDE; GENE EXPRESSION REGULATION, BACTERIAL; METABOLOME; RECOMBINANT PROTEINS; RIBOFLAVIN; RIBOSWITCH; SULFUR;

EID: 84946926580     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1515024112     Document Type: Article

References (24)

1. Fischer M, Bacher A (2005) Biosynthesis of flavocoenzymes. Nat Prod Rep 22(3): 324-350.
2. Mack M, van Loon AP, Hohmann HP (1998) Regulation of riboflavin biosynthesis in Bacillus subtilis is affected by the activity of the flavokinase/flavin adenine di-nucleotide synthetase encoded by ribC. J Bacteriol 180(4):950-955.
3. Fraaije MW, Mattevi A (2000) Flavoenzymes: Diverse catalysts with recurrent features. Trends Biochem Sci 25(3):126-132.
4. Macheroux P, Kappes B, Ealick SE (2011) Flavogenomics-A genomic and structural view of flavin-dependent proteins. FEBS J 278(15):2625-2634.
5. Mironov AS, et al. (2002) Sensing small molecules by nascent RNA: A mechanism to control transcription in bacteria. Cell 111(5):747-756.
6. Winkler WC, Cohen-Chalamish S, Breaker RR (2002) An mRNA structure that controls gene expression by binding FMN. Proc Natl Acad Sci USA 99(25):15908-15913.
7. Sklyarova S, Mironov A (2014) Bacillus subtilis ypaA gene regulation mechanism by FMN riboswitch. Russ J Genet 50(3):319-322.
8. Solovieva IM, Kreneva RA, Errais Lopes L, Perumov DA (2005) The riboflavin kinase encoding gene ribR of Bacillus subtilis is a part of a 10 kb operon, which is negatively regulated by the yrzC gene product. FEMS Microbiol Lett 243(1):51-58.
9. Burguière P, et al. (2005) Regulation of the Bacillus subtilis ytmI operon, involved in sulfur metabolism. J Bacteriol 187(17):6019-6030.
10. Coppée JY, et al. (2001) Sulfur-limitation-regulated proteins in Bacillus subtilis: A two-dimensional gel electrophoresis study. Microbiology 147(Pt 6):1631-1640.
11. Chan CM, Danchin A, Marlière P, Sekowska A (2014) Paralogous metabolism: S-alkyl-cysteine degradation in Bacillus subtilis. Environ Microbiol 16(1):101-117.
12. Higashitsuji Y, Angerer A, Berghaus S, Hobl B, Mack M (2007) Rib R, a possible regulator of the Bacillus subtilis riboflavin biosynthetic operon, in vivo interacts with the 5′-untranslated leader of rib mRNA. FEMS Microbiol Lett 274(1):48-54.
13. Vogl C, et al. (2007) Characterization of riboflavin (vitamin B2) transport proteins from Bacillus subtilis and Corynebacterium glutamicum. J Bacteriol 189(20):7367-7375.
14. Solovieva IM, Kreneva RA, Leak DJ, Perumov DA (1999) The ribR gene encodes a monofunctional riboflavin kinase which is involved in regulation of the Bacillus subtilis riboflavin operon. Microbiology 145(Pt 1):67-73.
15. Strohbach D, Novak N, Müller S (2006) Redox-active riboswitching: Allosteric regulation of ribozyme activity by ligand-shape control. Angew Chem Int Ed Engl 45(13): 2127-2129.
16. Mendoza-Vargas A, et al. (2009) Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli. PLoS One 4(10):e7526.
17. Pedrolli DB, Mack M (2014) Bacterial flavin mononucleotide riboswitches as targets for flavin analogs. Methods Mol Biol 1103:165-176.
18. Pedrolli DB, et al. (2012) A highly specialized flavin mononucleotide riboswitch responds differently to similar ligands and confers roseoflavin resistance to Strepto-myces davawensis. Nucleic Acids Res 40(17):8662-8673.
19. Fuhrer T, Heer D, Begemann B, Zamboni N (2011) High-throughput, accurate mass metabolome profiling of cellular extracts by flow injection-time-of-flight mass spec-trometry. Anal Chem 83(18):7074-7080.
20. Kanehisa M, et al. (2014) Data, information, knowledge and principle: Back to metabolism in KEGG. Nucleic Acids Res 42(Database issue):D199-D205.
21. Auger S, Danchin A, Martin-Verstraete I (2002) Global expression profile of Bacillus subtilis grown in the presence of sulfate or methionine. J Bacteriol 184(18): 5179-5186.
22. Even S, et al. (2006) Global control of cysteine metabolism by CymR in Bacillus subtilis. J Bacteriol 188(6):2184-2197.
23. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
24. Pedrolli D, et al. (2015) The ribB FMN riboswitch from Escherichia coli operates at the transcriptional and translational level and regulates riboflavin biosynthesis. FEBS J 282(16):3230-3242.