Low-cost generation of Good Manufacturing Practice-grade CD19-specific chimeric antigen receptor-expressing T cells using piggyBac gene transfer and patient-derived materials

Cytotherapy

Volumn 17, Issue 9, 2015, Pages 1251-1267

  Ramanayake, Saumya ^a   Bilmon, Ian ^a,b,c   Bishop, David ^a,b   Dubosq, Ming Celine ^a,b   Blyth, Emily ^a,b,c   Clancy, Leighton ^a,c   Gottlieb, David ^a,b,c   Micklethwaite, Kenneth ^a,b,c  

^a UNIVERSITY OF SYDNEY   (Australia)

^b WESTMEAD HOSPITAL   (Australia)

^c Sydney Cellular Therapies Laboratory   (Australia)

Author keywords

Chimeric antigen receptor; PiggyBac; T cells; Transposon

Indexed keywords

CD14 ANTIGEN; CD19 ANTIGEN; CD20 ANTIGEN; CD3 ANTIGEN; CD8 ANTIGEN; COMMON ACUTE LYMPHOBLASTIC LEUKEMIA ANTIGEN; GAMMA INTERFERON; INTERLEUKIN 15; INTERLEUKIN 2; INTERLEUKIN 7; CD19-SPECIFIC CHIMERIC ANTIGEN RECEPTOR; LYMPHOCYTE ANTIGEN RECEPTOR; TRANSPOSON;

ACUTE LYMPHOBLASTIC LEUKEMIA; ADOPTIVE TRANSFER; APOPTOSIS; ARTICLE; B CELL LYMPHOMA; CD19 SPECIFIC CHIMERIC ANTIGEN RECEPTOR T CELL; CELL EXPANSION; CELL STIMULATION; CHRONIC LYMPHATIC LEUKEMIA; COCULTURE; CONTROLLED STUDY; CYTOKINE PRODUCTION; CYTOKINE RELEASE; DNA MODIFICATION; ECONOMIC ASPECT; ELECTRICAL POTENTIAL PARAMETERS; ELECTROPORATION VOLTAGE; GENE TRANSFER; GOOD MANUFACTURING PRACTICE; HUMAN; HUMAN CELL; ICASP9 SUICIDE GENE; INCUBATION TIME; INTRACELLULAR SIGNALING; LEUKEMIA CELL LINE; LOW COST GENERATION; NONHODGKIN LYMPHOMA; PERIPHERAL BLOOD MONONUCLEAR CELL; PHENOTYPE; PIGGYBAC GENE TRANSFER; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; SUICIDE GENE; T LYMPHOCYTE; TRANSPOSON; ADULT; B LYMPHOCYTE; BIOSYNTHESIS; CELL SEPARATION; ELECTROPORATION; GENE VECTOR; GENETICS; IMMUNOLOGY; MALE; METABOLISM; PROCEDURES; TRANSPLANTATION;

ADOPTIVE TRANSFER; ADULT; ANTIGENS, CD19; B-LYMPHOCYTES; CELL SEPARATION; COCULTURE TECHNIQUES; DNA TRANSPOSABLE ELEMENTS; ELECTROPORATION; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HUMANS; INTERFERON-GAMMA; INTERLEUKIN-15; LEUKEMIA, LYMPHOCYTIC, CHRONIC, B-CELL; LYMPHOMA, NON-HODGKIN; MALE; PRECURSOR CELL LYMPHOBLASTIC LEUKEMIA-LYMPHOMA; RECEPTORS, ANTIGEN, T-CELL; T-LYMPHOCYTES;

EID: 84946475107     PISSN: 14653249     EISSN: 14772566     Source Type: Journal    
DOI: 10.1016/j.jcyt.2015.05.013     Document Type: Article

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